Pesticide omethoate degrading microorganism compound bacterial agent and preparation method thereof

A technology of omethoate and compound bacterial agent, which is applied in the field of soil bioremediation, can solve problems such as difficult degradation, and achieve the effects of simple construction, protection of agricultural product safety, and no secondary pollution

Active Publication Date: 2019-12-20
MICROBIOLOGY INST OF SHAANXI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Theoretically, organophosphorus pesticides in the environment are easy to decompose organic substances and are not easy to remain, but the intermediate degradation products are persistent pollutants, which are difficult to be completely degraded

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0047] The preparation method of the above-mentioned pesticide omethoate degrading microbial compound bacterial agent comprises the following steps:

[0048] Step 1: For Pseudomonas Gram ( Pseudomonas graminis ) ZZY-C13-1-8 cells were cultured in seed liquid to obtain Pseudomonas gramii ( Pseudomonas graminis ) ZZY-C13-1-8 seed solution;

[0049] against Pseudomonas ayucida ( Pseudomonas plecoglossicida ) cells were cultured in seed liquid to obtain Pseudomonas ayucididae ( Pseudomonas plecoglossicida ) seed liquid;

[0050] Step 2: To Pseudomonas gramei ( Pseudomonas graminis ) ZZY-C13-1-8, Ayucidal Pseudomonas ( Pseudomonas plecoglossicida ) for liquid fermentation;

[0051] Step 3: centrifuging the liquid fermented product respectively to obtain liquid fermented cell A and cell B;

[0052] Step 4: Mix thalline A, thalline B with glycerin, activated carbon, and kaolin at a mass ratio of 1:1:(1.5-2):3:(2.5-3) to obtain the pesticide omethoate-degrading microbial com...

Embodiment 1

[0073] Step 1: Seed liquid culture

[0074] Prepare liquid medium a, insert Pseudomonas gramii ZZY-C13-1-8 cells into medium a aseptically, place at 28°C, 160rpm, shake the flask for 72 hours, and put it into the fermentation broth The cells were grown to the logarithmic phase, and the seed solution of Pseudomonas ZZY-C13-1-8 was obtained;

[0075] The formula of liquid fermentation medium a is:

[0076] 2.5 grams of yeast extract powder, 5 grams of peptone, 2.5 milliliters of glycerin, 2.5 grams of NaCl, 2.5 grams of potassium dihydrogen phosphate, add water to 500 milliliters.

[0077] Prepare liquid medium b, insert Pseudomonas ayucidal bacteria into medium b aseptically, place at a temperature of 28°C, 160rpm, shake the flask for 48 hours, until the cells in the fermentation broth grow to logarithmic phase, Obtain Pseudomonas seed liquid;

[0078] The formula of liquid fermentation medium b is:

[0079] Yeast extract powder 2.5 grams, peptone 2.5 grams, glycerin 5 mill...

Embodiment 2

[0092]Step 1 Preparation of seed A: using liquid fermentation medium a, aseptically insert Pseudomonas gramis ZZY-C13-1-8 bacteria into medium a, place at 30°C, 180rpm, shake flask culture 60 hours, until the cells in the fermentation broth grow to the logarithmic phase, and obtain the Pseudomonas ZZY-C13-1-8 seed solution;

[0093] The formula of liquid fermentation medium a is:

[0094] 2.5 grams of yeast extract powder, 2.5 grams of peptone, 5 milliliters of glycerin, 2.5 grams of NaCl, 5 grams of potassium dihydrogen phosphate, add water to 500 milliliters.

[0095] Seed B preparation: using liquid fermentation medium b, aseptically insert Pseudomonas ayucidal bacteria into medium b, place at a temperature of 30°C, 180rpm, shake the flask for 48 hours, until the cells grow in the fermentation broth To logarithmic phase, obtain Pseudomonas seed solution;

[0096] The formula of liquid fermentation medium b is:

[0097] 5 grams of yeast extract powder, 5 grams of peptone,...

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Abstract

The invention relates to a pesticide omethoate degrading microorganism compound bacterial agent and a preparation method thereof. The compound bacterial agent is prepared from Pseudomonas graminis ZZY-C13-1-8 or a mixture of the Pseudomonas graminis ZZY-C13-1-8 and Pseudomonas plecoglossicida, glycerin, activated carbon and kaoline with a mixing ratio being 1 to 1 to (1.5-2) to 3 to (2.5-3). A thallus involved by the invention is cultured by a special fluid nutrient medium, the obtained compound bacterial agent can degrade omethoate residual in agricultural soils in situ with the degrading ratio of the omethoate residual in the soils reaching 75 percent or above, and the pesticide omethoate degrading microorganism compound bacterial agent and the preparation method have the advantages of low treatment cost, simpleness in construction, no influence on production and operation, no secondary pollution, small influence on environment and the like.

Description

technical field [0001] The invention belongs to the technical field of soil bioremediation, and in particular relates to a pesticide omethoate-degrading microbial composite bacterial agent and a preparation method thereof. Background technique [0002] As an important means to ensure a good agricultural harvest, chemical pesticides play an important role in agricultural production. The main problems in the use of chemical pesticides are irrational use of chemical pesticides, serious pesticide residues in agricultural products, high control costs, serious pollution to the ecological environment, and large-scale phytotoxicity. [0003] Microbial remediation is a biological measure that uses the metabolism of specific microorganisms to absorb, transform, remove or degrade chemical pesticide components to achieve environmental purification and ecological effect recovery. It has the characteristics of high efficiency, economy, and no secondary pollution. This technology has been...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/38
CPCC12N1/20B09C1/10
Inventor 陈锐瞿佳孙晓宇门欣赵玲侠沈卫荣
Owner MICROBIOLOGY INST OF SHAANXI
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