Pesticide omethoate degrading microorganism compound bacterial agent and preparation method thereof
A technology of omethoate and compound bacterial agent, which is applied in the field of soil bioremediation, can solve problems such as difficult degradation, and achieve the effects of simple construction, protection of agricultural product safety, and no secondary pollution
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[0047] The preparation method of the above-mentioned pesticide omethoate degrading microbial compound bacterial agent comprises the following steps:
[0048] Step 1: For Pseudomonas Gram ( Pseudomonas graminis ) ZZY-C13-1-8 cells were cultured in seed liquid to obtain Pseudomonas gramii ( Pseudomonas graminis ) ZZY-C13-1-8 seed solution;
[0049] against Pseudomonas ayucida ( Pseudomonas plecoglossicida ) cells were cultured in seed liquid to obtain Pseudomonas ayucididae ( Pseudomonas plecoglossicida ) seed liquid;
[0050] Step 2: To Pseudomonas gramei ( Pseudomonas graminis ) ZZY-C13-1-8, Ayucidal Pseudomonas ( Pseudomonas plecoglossicida ) for liquid fermentation;
[0051] Step 3: centrifuging the liquid fermented product respectively to obtain liquid fermented cell A and cell B;
[0052] Step 4: Mix thalline A, thalline B with glycerin, activated carbon, and kaolin at a mass ratio of 1:1:(1.5-2):3:(2.5-3) to obtain the pesticide omethoate-degrading microbial com...
Embodiment 1
[0073] Step 1: Seed liquid culture
[0074] Prepare liquid medium a, insert Pseudomonas gramii ZZY-C13-1-8 cells into medium a aseptically, place at 28°C, 160rpm, shake the flask for 72 hours, and put it into the fermentation broth The cells were grown to the logarithmic phase, and the seed solution of Pseudomonas ZZY-C13-1-8 was obtained;
[0075] The formula of liquid fermentation medium a is:
[0076] 2.5 grams of yeast extract powder, 5 grams of peptone, 2.5 milliliters of glycerin, 2.5 grams of NaCl, 2.5 grams of potassium dihydrogen phosphate, add water to 500 milliliters.
[0077] Prepare liquid medium b, insert Pseudomonas ayucidal bacteria into medium b aseptically, place at a temperature of 28°C, 160rpm, shake the flask for 48 hours, until the cells in the fermentation broth grow to logarithmic phase, Obtain Pseudomonas seed liquid;
[0078] The formula of liquid fermentation medium b is:
[0079] Yeast extract powder 2.5 grams, peptone 2.5 grams, glycerin 5 mill...
Embodiment 2
[0092]Step 1 Preparation of seed A: using liquid fermentation medium a, aseptically insert Pseudomonas gramis ZZY-C13-1-8 bacteria into medium a, place at 30°C, 180rpm, shake flask culture 60 hours, until the cells in the fermentation broth grow to the logarithmic phase, and obtain the Pseudomonas ZZY-C13-1-8 seed solution;
[0093] The formula of liquid fermentation medium a is:
[0094] 2.5 grams of yeast extract powder, 2.5 grams of peptone, 5 milliliters of glycerin, 2.5 grams of NaCl, 5 grams of potassium dihydrogen phosphate, add water to 500 milliliters.
[0095] Seed B preparation: using liquid fermentation medium b, aseptically insert Pseudomonas ayucidal bacteria into medium b, place at a temperature of 30°C, 180rpm, shake the flask for 48 hours, until the cells grow in the fermentation broth To logarithmic phase, obtain Pseudomonas seed solution;
[0096] The formula of liquid fermentation medium b is:
[0097] 5 grams of yeast extract powder, 5 grams of peptone,...
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