78 results about "Arginine deiminase" patented technology

The present invention is directed to methods of modulating viral replication in vivo comprising administering to an individual a therapeutically or prophylactically effective amount of a composition comprising arginine deiminase modified with polyethylene glycol, to methods of concurrently modulating viral replication and treating cancer, and to methods of modulating nitric oxide levels in a patient, among others.

The present invention is directed to arginine deiminase modified with polyethylene glycol, to methods of treating cancer, and to methods of treating and / or inhibiting metastasis.

The present invention is directed to methods of modulating viral replication in vivo comprising administering to an individual a therapeutically or prophylactically effective amount of a composition comprising arginine deiminase modified with polyethylene glycol, to methods of concurrently modulating viral replication and treating cancer, and to methods of modulating nitric oxide levels in a patient, among others.

The invention discloses a method to produce ADI strain by screening and the application of the method, pertaining to the field of bioengineering technology. The invention particularly relates to a rapid method to screen ADI strain and produce Pseudomonas plecoglossicida (CGMCC No.2039) of ADI, as well as a method to produce ADI by using the microorganism. Under an aerobic condition and an environment maintained at pH6.5-8.0, enzyme activity can achieve 1.6U / mL fermentation broth after 20-24h fermentation culture. ADI produced with such microorganism has very strong inhabitation agaisnt liver cancer cell strain (HepG2). ADI genes can be obtained from the chromosome DNA of such microorganism, and the gene order can be achieved.

The invention relates to a strain of gene engineering arginine deiminase reformed through site directed mutagenesis, and belongs to the technical field of gene engineering, wherein the amino acid sequence is SEQ ID No.1, and the 264th site glycine in the amino acid sequence of the arginine deiminase reformed through site directed mutagenesis mutates into the proline relative to the amino acid sequence of the natural arginine deiminase. According to the present invention, compared to the wild enzyme, the mutant arginine deiminease has the following characteristics that the effective pH value action range is expanded to a certain degree, particularly the good enzyme activity is provided at the physiological pH value of 7.4, and the mutant enzyme has the high stability under the pH value of 5.5-7.5 along with the broadening of the effective pH value action range, such that the problems of low enzyme activity and short half-life in vivo under the physiological conditions of the clinical applications of the arginine deiminase in tumor treatment are solved, and the good conditions are created for the applications of the arginine deiminase and the coding gene thereof in the clinical treatment.

The present invention relates generally to methods of treating cancer with arginine deiminase, and in particular pegylated arginine deiminase.

The invention discloses a recombination preparation method of arginine deiminase (hereinafter referred to as ADI), and particularly discloses the preparation method for escherichia coli to conduct recombination expression on the ADI. The method mainly comprises a recombination expression method and an inclusion body renaturation method using urea as a denaturing agent. The N tail end of the ADI prepared through recombination expression does not contain methionine.

The invention discloses a PEG (polyethylene glycol) modified recombinant arginine deiminase (ADI) as well as a preparation method and application thereof, belonging to the technical field of biomedical engineering. The stability of ADI-PEG obtained by modifying ADI with PEG in vitro and in mouse plasma is remarkably improved. A pharmacokinetics / pharmacodynamics study shows that compared with free ADI before modification, the half-life period of ADI-PEG in a mouse is prolonged by more than 11 times; the concentration of arginine in the plasma can be reduced and maintained for more than 5 days in the limiting level through single injection of 5U ADI-PEG and can be maintained for 1 day only through injection of unmodified ADI; an H22 liver cancer model mouse is treated for 15 days, and the cancer cell inhibition rate of ADI-PEG (15U) is 95.02% close to 98.34% (the inhibition rate) of a positive control group treated with 5-fluorouracil. PEG modified recombinant arginine deiminase is relatively long in in-vivo half-life period and relatively low in immunogenicity, and serves as an excellent tumor treating or inhibiting drug.

The present invention relates to a pharmaceutical composition for inhibiting angiogenesis which comprises arginine deiminase as an active ingredient, where the arginine deiminase, obtained from Mycoplasma arginini or prepared by a genetic recombination technique, may be conjugated to an activated polymer to lower its immunogenecity and increase its life time. The pharmaceutical composition of the present invention exhibits an excellent inhibitory activity against angiogenesis.

The invention belongs to the field of pharmaceutical preparations. The invention relates to a preparation method and application of a macrophage membrane coated arginine deiminase / catalase / IR780 nanoparticle. The prepared macrophage membrane coated arginine deiminase / catalase / IR780 nanoparticle is good in biocompatibility, the preparation method is simple, the particle size distribution of the nanoparticle is uniform, the stability of the arginine deiminase can be improved, the protease hydrolysis resistance ability of the arginine deiminase is improved, an anti-tumor effect is enhanced in cooperation with photothermal therapy or photodynamic therapy, and a potential application value is achieved in the fields of treatment of cancers (especially arginine-dependent diseases) and the like.

The present invention relates generally to methods of treating cancer with arginine deiminase, and in particular pegylated arginine deiminase.

The invention discloses a method to produce ADI strain by screening and the application of the method, pertaining to the field of bioengineering technology. The invention particularly relates to a rapid method to screen ADI strain and produce Pseudomonas plecoglossicida (CGMCC No.2039) of ADI, as well as a method to produce ADI by using the microorganism. Under an aerobic condition and an environment maintained at pH6.5-8.0, enzyme activity can achieve 1.6U / mL fermentation broth after 20-24h fermentation culture. ADI produced with such microorganism has very strong inhabitation agaisnt liver cancer cell strain (HepG2). ADI genes can be obtained from the chromosome DNA of such microorganism, and the gene order can be achieved.