A kind of Pseudomonas mutans and application thereof

A technology of Pseudomonas mutans and bacterial preparations, which is applied to Pseudomonas mutans and its application fields to achieve the effects of improving biodegradability and reducing chemical oxygen demand and biochemical oxygen demand

Active Publication Date: 2020-08-07
BC P INC CHINA NAT PETROLEUM CORP +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there is currently no bioaugmentation method for the degradation of nitrile compounds in acrylonitrile wastewater

Method used

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  • A kind of Pseudomonas mutans and application thereof
  • A kind of Pseudomonas mutans and application thereof
  • A kind of Pseudomonas mutans and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] In the present invention, Pseudomonas plecoglossicida (Pseudomonas plecoglossicida) BD is screened from the outlet sludge of the activated sludge treatment process A of the sewage workshop of PetroChina Dalian Petrochemical Company. The specific process is as follows:

[0047] Step 1). Take activated sludge from the outlet of the activated sludge treatment process A pool of the sewage workshop of PetroChina Dalian Petrochemical Company;

[0048] Step 2). Inoculate 5 g of activated sludge into an inorganic salt medium with a volume fraction of acrylonitrile of 0.5%, and cultivate for 7 days on a shaker with a temperature of 35°C and a rotation speed of 150 rpm to obtain the first bacterial solution ;

[0049] Step 3). Transfer the first bacterial solution in step 2) to an inorganic salt medium with a volume fraction of acrylonitrile of 1.5%, and cultivate for 7 days on a shaker with a temperature of 35°C and a rotation speed of 150 rpm. Obtain the second bacterial liquid.

[0...

Embodiment 2

[0054] The strains screened in Example 1 were identified.

[0055] In Example 1 of the present invention, three strains were screened, which were named BD, DR, and DW-2 in the present invention. Among them, BD was identified as Pseudomonas plecoglossicida.

[0056] Pseudomonas plecoglossicida BD grows well on agar plate medium, such as figure 1 As shown, the colony is milky white, round, raised, opaque, and of different sizes, with a diameter of 3 to 4 mm, a moist surface, and a smooth edge. The cells of strain BD are rod-shaped, with a size of 0.5-2.8 μm, and Gram staining is negative, with motility and facultative anaerobic growth. The growth temperature of the strain is 20-40°C and pH 6-9. Optimum growth conditions: growth temperature is 35 ℃, pH 7.5. Can use glucose but cannot use starch.

[0057] DNA was extracted from the single colony obtained in step 5) in Example 1, and DNA sequencing was performed on the bacterial species. In the present invention, a strain is obtained...

Embodiment 3

[0059] The organic matter degradation ability of Pseudomonas plecoglossicida BD obtained in Example 1 and DR and DW-2 strains screened at the same time were measured. Specific steps are as follows:

[0060] Step 1). Take the Pseudomonas plecoglossicida BD obtained in Example 1, centrifuge at 3000 rpm for 10 minutes, discard the supernatant, and add distilled water to the pellet to resuspend at 3000 rpm Centrifuge for 10 minutes and repeat 3 times to obtain Pseudomonas plecoglossicida BD;

[0061] Step 2). Add 0 mL, 8.2 mL, 4.92 mL, and 1.64 mL of Pseudomonas plecoglossicida BD to the Biochemical Oxygen Demand (BOD) test numbered 1, 2, 3, and 4, respectively And add acrylonitrile wastewater with a chemical oxygen demand (COD) of 404.8 mg / L to each BOD test bottle to a total volume of 164 mL. The deformations in No. 1 (control), 2, 3, and 4 The concentration of Pseudomonasplecoglossicida BD strain is 8.1×10 7 ~6.4×10 8 cell / mL, the volume fractions are 0, 5%, 3%, and 1% respectivel...

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Abstract

The invention discloses a Pseudomonas plecoglossicida and an application thereof, and specifically provides a Pseudomonas plecoglossicida BD, whose preservation number is CGMCC No.14205. The embodiment of the present invention provides Pseudomonas plecoglossicida (Pseudomonas plecoglossicida) BD, which can grow and reproduce with nitrile compounds as the only energy source, can degrade pollutants such as nitrile compounds in acrylonitrile wastewater, and reduce the chemical oxygen demand of acrylonitrile wastewater Amount and biochemical oxygen demand, thereby improving the biodegradability of acrylonitrile wastewater.

Description

Technical field [0001] The invention relates to a Pseudomonas mutans and its applications, in particular to a Pseudomonas plecoglossicida that can degrade nitrile compounds in acrylonitrile wastewater and its related applications. Background technique [0002] Acrylonitrile wastewater usually comes from acrylonitrile production wastewater, acrylic fiber production wastewater, and acrylonitrile-butadiene-styrene (ABS) plastic production wastewater. Acrylonitrile wastewater contains a large amount of acrylonitrile, acrylonitrile dimer, acetonitrile, mononuclear aromatic hydrocarbons, and acrylonitrile derivatives (such as acrolein, acetone cyanohydrin, methyl acrylate, acrylamide, dimethylformamide), etc. Among them, the nitrile compounds in the acrylonitrile wastewater are highly toxic substances. If the acrylonitrile wastewater is directly discharged, it will definitely cause great harm to the environment, so the treatment of the acrylonitrile wastewater is very important. [0003...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C02F3/28C12R1/38C02F101/38
CPCC02F3/2853C02F3/348C02F2101/38C12N1/20C12N1/205C12R2001/38
Inventor 董红红梁传富张北屿孙珊珊王伟许谦吴云蕊贾凡凹张忠智
Owner BC P INC CHINA NAT PETROLEUM CORP
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