Pseudomonas plecoglossicida and application thereof

A technology of Pseudomonas mutans and strains, applied in Pseudomonas mutans and its application fields, to achieve the effect of improving biodegradability, reducing chemical oxygen demand and biochemical oxygen demand

Active Publication Date: 2017-11-28
BC P INC CHINA NAT PETROLEUM CORP +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there is currently no bioaugmentation method for the degradation of nitrile compounds in acrylonitrile wastewater

Method used

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  • Pseudomonas plecoglossicida and application thereof
  • Pseudomonas plecoglossicida and application thereof
  • Pseudomonas plecoglossicida and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] In the present invention, Pseudomonas plecoglossicida (Pseudomonas plecoglossicida) BD is screened from the sludge at the outlet of Pool A of the activated sludge method treatment process in the wastewater workshop of PetroChina Dalian Petrochemical Company. The specific process is as follows:

[0047] Step 1), take activated sludge from the outlet of pool A of the activated sludge method treatment process in the sewage workshop of PetroChina Dalian Petrochemical Company;

[0048] Step 2), inoculating 5 g of activated sludge into an inorganic salt medium with acrylonitrile volume fraction of 0.5%, cultivating on a shaking table with a temperature of 35° C. and a rotation speed of 150 rpm for 7 days to obtain the first bacterial liquid ;

[0049] Step 3), absorbing the first bacterial solution in step 2) and transferring it to an inorganic salt medium with a volume fraction of acrylonitrile of 1.5%, culturing on a shaker at a temperature of 35° C. and a rotation speed o...

Embodiment 2

[0054] The strains screened in Example 1 were identified.

[0055] In Example 1 of the present invention, three strains were screened, which were named BD, DR, and DW-2 in the present invention. Among them, BD was identified as Pseudomonas plecoglossicida.

[0056] Pseudomonas plecoglossicida (Pseudomonas plecoglossicida) BD grows well on agar plate medium, such as figure 1 As shown, the colony is milky white round, raised, opaque, of different sizes, 3-4mm in diameter, moist on the surface, and flat on the edge. The cells of strain BD are rod-shaped, 0.5-2.8 μm in size, Gram staining is negative, motile, facultative anaerobic growth. The growth temperature of the strain is 20-40°C, and the pH is 6-9. Optimum growth conditions: growth temperature is 35°C, pH 7.5. Glucose can be used, but starch cannot be used.

[0057] Extract DNA from the single colony obtained in step 5) in Example 1, and perform DNA sequencing on the bacterial species. In the present invention, a bact...

Embodiment 3

[0059] The organic matter degradation ability of Pseudomonas plecoglossicida (Pseudomonas plecoglossicida) BD obtained in Example 1 and DR and DW-2 bacteria screened at the same time was determined. Specific steps are as follows:

[0060] Step 1), get the bacterium liquid of the Pseudomonas plecoglossicida (Pseudomonas plecoglossicida) BD obtained in Example 1, centrifuge at 3000 rpm for 10 minutes, discard the supernatant, add distilled water to the precipitate for resuspension, and spin at 3000 rpm Centrifuge for 10 minutes and repeat 3 times to obtain Pseudomonas plecoglossicida BD;

[0061] Step 2), adding 0mL, 8.2mL, 4.92mL, and 1.64mL of Pseudomonas plecoglossicida (Pseudomonas plecoglossicida) BD into the biochemical oxygen demand (Biochemical OxygenDemand, referred to as BOD) test numbered 1, 2, 3, 4 respectively bottle, and adding chemical oxygen demand (COD) to each BOD test bottle is 404.8mg / L of acrylonitrile waste water to a total volume of 164mL, the deformed fa...

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Abstract

The invention discloses pseudomonas plecoglossicida and application thereof and particularly provides pseudomonas plecoglossicida BD with the preservation number being CGMCC No.14205. The provided pseudomonas plecoglossicida BD can grow and propagate with nitrile compounds as the only energy source, can degrade contaminants such as the nitrile compounds in acrylonitrile waste water and reduces the chemical oxygen demand and the biochemical oxygen demand of the acrylonitrile waste water, and accordingly the biodegradability of the acrylonitrile waste water is improved.

Description

technical field [0001] The present invention relates to a kind of Pseudomonas plecoglossicida and application thereof, in particular to a Pseudomonas plecoglossicida which can degrade nitrile compounds in acrylonitrile waste water and related applications. Background technique [0002] Acrylonitrile wastewater usually comes from the production wastewater of acrylonitrile, the production wastewater of acrylic fibers, and the production wastewater of acrylonitrile-butadiene-styrene (ABS) plastics. Acrylonitrile wastewater contains a large amount of acrylonitrile, acrylonitrile dimer, acetonitrile, mononuclear aromatics, and acrylonitrile derivatives (such as acrolein, acetone cyanohydrin, methyl acrylate, acrylamide, dimethylformamide), etc. Among them, the nitrile compounds in acrylonitrile wastewater are highly toxic substances. If the acrylonitrile wastewater is directly discharged, it will definitely cause great harm to the environment, so the treatment of acrylonitrile wa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C02F3/28C12R1/38C02F101/38
CPCC02F3/2853C02F3/348C02F2101/38C12N1/20C12N1/205C12R2001/38
Inventor 董红红梁传富张北屿孙珊珊王伟许谦吴云蕊贾凡凹张忠智
Owner BC P INC CHINA NAT PETROLEUM CORP
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