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An aerobic denitrifying bacteria and its application

An aerobic denitrification and bacteria technology, applied in the field of environmental microorganisms, can solve the problems of high operating cost, complicated operation and large energy consumption.

Inactive Publication Date: 2018-09-25
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional biological denitrification has been widely recognized due to its good denitrification efficiency, but because it consists of two processes, aerobic nitrification and anaerobic / anoxic denitrification, it also causes complex operations, high energy consumption and high operating costs. shortcoming

Method used

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  • An aerobic denitrifying bacteria and its application
  • An aerobic denitrifying bacteria and its application
  • An aerobic denitrifying bacteria and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: Isolation of aerobic denitrification bacterial strain YIM DC16

[0024] (1) The medium and components used are as follows:

[0025] Enrichment medium (g / L): KNO 3 2 g, K 2 HPO 4 1 g, KH 2 PO 4 1 g, MgSO 4 ·7H 2 O 0.2 g, sodium citrate 5 g, trace salt solution 2 mL; distilled water 1000 mL, adjust pH 7.0-7.2 with 1N NaOH;

[0026] Among them, trace salt solution (g / L): EDTA 50 g, ZnSO 4 ·7H 2 O 22 g, CaCl 2 5.54 g, MnCl 2 4H 2 O5.06 g, FeSO 4 ·7H 2 O 4.99 g, (NH 4 ) 6 Mo 7 o 24 4H 2 O 1.10 g, CuSO 4 ·5H 2 O 1.57 g, CoCl 2 ·6H 2 O 1.61 g.

[0027] BTB medium (g / L): bromothymol blue (BTB) selective medium, agar 15 g, KNO 3 1g, KH 2 PO 4 1 g, FeCl 2 ·6H 2 O 0.5 g, CaCl 2 ·7H 2 O 0.2 g, MgSO 4 ·7H 2 O 1 g, sodium succinate 8.5 g, BTB (1% dissolved in alcohol) 1 mL; distilled water 1000 mL, adjust pH 7.0-7.2 with 1N NaOH.

[0028] LB medium (g / L): 10 g peptone, 5 g NaCl, 5 g yeast extract, 15 g agar.

[0029] Denitrificati...

Embodiment 2

[0036] Embodiment 2: Molecular biology identification of bacterial strain YIM DC16 bacterial strain

[0037] (1) Colony morphological characteristics: The colony morphological characteristics of YIM DC16 cultured on LB medium for 24 h were round, smooth and milky white. Gram-stain negative, the cells are rod-shaped with flagella (see figure 1 ).

[0038] (2) Use Takara's Lysis Buffer to extract the genomic DNA of strain YIM DC16, use it as a template, PCR amplify its 16S rRNA gene, separate the PCR product by 1% agarose gel electrophoresis, and pass through the gel recovery kit Perform DNA purification. The 16S rRNA gene fragment of strain YIM DC16 was transformed into E. coli In DH5α, positive clones were selected and sent to Shanghai Sangong for sequence determination. The sequence is shown in the sequence table as SEQ ID NO: 1; the sequencing results were compared and analyzed with the 16S rRNA gene sequences in the GenBank and Eztaxon databases to obtain the identiti...

Embodiment 3

[0042] Example 3: Effects of different environmental factors on denitrification performance of aerobic denitrification strain YIM DC16

[0043] Basal medium: KNO 3 0.6 g, KH 2 PO 4 1 g, K 2 HPO 4 1 g, MgSO 4 ·7H 2 O 0.2 g, deionized water 1000 mL.

[0044] The effect of different carbon sources on the denitrification efficiency of strains: glucose, sucrose, sodium acetate, sodium succinate, sodium citrate, sodium tartrate and methanol were used as the only carbon source to test the denitrification effect, under the conditions of 30 ℃ and 160 rpm Cultivate for 48 h. The results showed that aerobic denitrifying bacteria did not have good denitrification effects in sucrose and methanol media, but had better denitrification effects in glucose, sodium succinate, sodium citrate, sodium acetate and sodium tartrate media, especially In glucose, sodium citrate and sodium tartrate medium, the removal rate of nitrate was above 95%, and in the medium with glucose as carbon sour...

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Abstract

The invention discloses a strain of aerobic denitrifying bacterium Pseudomonas plecoglossicida YIM DC16, which has been preserved in the General Microorganism Center of the China Microorganism Culture Collection and Management Committee on July 14, 2015, and the preservation number is CGMCC No.11077; it is a microbial strain with aerobic denitrification ability; the strain of the present invention can utilize a variety of carbon sources such as glucose, sodium succinate, sodium citrate, sodium acetate, sodium tartrate and the like under aerobic or oxygen-limited conditions. Aerobic denitrification; the strain was cultured at 20°C, 100 rpm, initial pH 7.5, and C / N=15 in a liquid medium with glucose as the carbon source and KNO3 as the sole nitrogen source for 36 h. The removal rate of nitrate nitrogen can reach 90.07%, and the removal rate of total nitrogen can also reach 76.99%, which has a good market application prospect.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to an aerobic denitrification bacterial strain and application thereof. Background technique [0002] Nitrogen is an essential component for animal protein synthesis and plant growth, and is one of the most important nutrients for organisms. However, with the expansion of cities and the development of industry and agriculture, humans excessively release nitrogen-containing compounds into water bodies, exceeding the carrying capacity of the environment, which not only causes water eutrophication and algal bloom risks, but also reduces oxygen content and biodiversity , causing various impacts such as damage to the water ecosystem, and also seriously affecting human health. Therefore, people pay more and more attention to the control of nitrogen pollution and the innovation of nitrogen removal process. [0003] The denitrification process can be divided into ph...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/38C02F101/38
CPCC02F3/34C02F2101/38C12N1/205C12R2001/38
Inventor 霍晴晴王永霞李亚平肖炜赖泳红李治滢和树庄崔晓龙
Owner YUNNAN UNIV
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