545 results about "Citrate sodium" patented technology

The invention provides a method for grafting Tibetan white bark pine on Chinese pine, belongs to the technical field of needle-leaved tree species grafting, and solves the technical problem that the survival rate is lower than 40% due to slow joint healing, high pathogen infection rate and environmental fluctuation. According to the technical scheme, the method is characterized by comprising the following steps: cutting 1.8-2.2 cm of a vertical notch reaching xylem at the part, which is 2.5-3.5 cm exposed out of the surface of a culture medium, of a rootstock rhizome part; cutting the scion base into a symmetrical double-sided wedge shape, and immersing the scion base into a sodium citrate buffer solution containing polyvinylpyrrolidone, methyl salicylate and calcium chloride; the two sides of the vertical notch are coated with adsorption glue; inserting a scion alignment cambium and fixing by using a grafting belt; an ethanol water solution containing salicylic acid, trehalose and polysorbate 80 is applied to the interface every 2-3 days; maintaining the day temperature to be 23-25 DEG C, the night temperature to be 16-18 DEG C and the humidity to be 90-95%; when the young sprouts grow to 2-3 cm, the rootstocks are girdled, and trunks are cut off after 7-10 days. The method is used for large-scale breeding of the Tibetan pinus bungeana grafted seedlings.

The invention relates to the field of inorganic material preparation, and particularly discloses pseudo-boehmite and a preparation method thereof. A preparation method of pseudo-boehmite comprises the following steps: (1) mixing an aluminum salt solution and an aluminate solution, adding a crystal form regulating agent, and stirring for reaction to obtain a sol system; the crystal form regulating agent is ZnS quantum dots modified by thiohydracrylic acid; (2) adding a pore diameter guiding agent into the sol system, and aging for 6-8 hours; the pore diameter guiding agent is a compound of a polyethylene glycol-polypropylene glycol-polyethylene glycol triblock copolymer and sodium citrate; and (3) carrying out solid-liquid separation on the aged sol system, and washing and drying the obtained solid to obtain the pseudo-boehmite. The proportion of 5-10nm pores of the pseudo-boehmite prepared by the preparation method disclosed by the invention reaches 90% or above, so that the pore distribution requirement of a fine catalytic reaction on the pseudo-boehmite can be met, and the efficient catalytic reaction is favorably realized.

The invention discloses a bone cement composition and application thereof, and relates to the technical field of bone cement. The bone cement composition comprises a solid phase component and a liquid phase component, wherein the solid phase component comprises 60-75 parts of composite calcium phosphate, 10-20 parts of strontium glass powder, 8-12 parts of a reinforcing agent and 3-7 parts of a solidification reaction regulator; the liquid phase component comprises 20-30 parts of a phosphate buffer solution, 3-8 parts of a sodium citrate solution, 5-15 parts of an anti-inflammatory agent and 1-5 parts of a hyaluronic acid solution. The preparation method comprises the following steps: reacting 1, 10-phenanthrene-2, 9-dicarboxylic acid with 2-aminomethyl furan to obtain an intermediate 1, and reacting the intermediate 1 with poly (ethylene glycol) maleimide to prepare the reinforcing agent. The prepared bone cement composition has good mechanical properties, self-repairing performance and anti-inflammatory performance, and has a good application prospect in the field of bone repairing.

The invention discloses a preparation method and application of autologous fibrin glue, and belongs to the field of biomedical materials. The preparation method comprises the following five steps: collecting autologous venous blood, adding a sodium citrate anticoagulant with the volume ratio of 1: (8-12), and storing at 4-6 DEG C for not more than 2 hours; performing step-by-step centrifugation at the speed of 1500 to 2000 r / min and the speed of 3500 to 4000 r / min to obtain a fibrinogen concentrate; the thrombin-calcium chloride activation solution A and the tranexamic acid activation solution B are mixed according to the ratio of 1: (2-3) to form a composite activation system; mixing the two components according to a mass ratio of 1: (0.8-1.2), and reacting at 25-37 DEG C for 3-5 minutes to form colloid; and pre-freezing at-40 to-60 DEG C, performing vacuum freeze-drying, and storing at 4-8 DEG C. During application, the colloid can be coated on an operation wound surface after being redissolved, or mixed with a growth factor to prepare a dressing for covering a chronic wound surface. The colloid is high in purity, stable in coagulation time, long in storage period, suitable for scenes such as surgical hemostasis and the like, and high in clinical value.

The invention relates to the technical field of corrosion prevention of oil field pipes, and particularly discloses electroplating liquid for a high-tungsten-content hydrogen sulfide corrosion resistant sleeve coating and a preparation process of the electroplating liquid. The invention discloses electroplating liquid for a high-tungsten-content hydrogen sulfide corrosion-resistant sleeve coating. The electroplating liquid comprises the following raw materials: 60-90 parts of a composite tungsten source; 40-70 parts of a nickel-cobalt main salt; 35-55 parts of a composite complexing agent; 1-5 parts of carboxylated graphene quantum dots; 1 to 5 parts of an aminated ZIF-8 derived carbon nanocage; 1-5 parts of a rare earth stabilizer; 1-4 parts of sodium hypophosphite; 1-4 parts of sodium citrate; 1-3 parts of a surfactant; 15 to 25 parts of a buffering agent; and 350 to 450 parts of deionized water. According to the single-layer electroplated layer, the interface stress of the multi-layer structure can be eliminated, so that other interface problems such as plating layer cracking caused by underground temperature fluctuation are avoided, it is guaranteed that the thickness of the plating layer reaches the standard, and then it is guaranteed that the plating layer has excellent corrosion resistance.

The invention relates to a preparation method of a biomass-based degradable dust suppressant and a spraying device. The biomass-based degradable dust suppressant is prepared from the following components in percentage by weight: 0.78% of a biological matrix, 20.7% of a salting-out salt solution and the balance of an acetic acid solution diluted by deionized water, the method comprises the following specific steps: adding biological matrix powder into an acetic acid solution; the salting-out salt solution powder is poured into a biological matrix acetic acid solution, magnetic stirring is conducted for 30 min under the conditions that the rotating speed is 200 r / min and the temperature is 30 DEG C, and then ultrasonic treatment is conducted for 10 min for full reaction; a salting-out method and a dust suppression technology are combined, high-concentration salt solutions such as sodium citrate and sodium sulfate are selected, physical and chemical effects between salt ions and biomass material molecules are utilized, and the limitation of a traditional cross-linking mode is broken through the synergistic effect of salting-out auxiliary precipitation and weak ion cross-linking net forming; according to the present invention, the long-acting and safe dust suppression agent can be simply and conveniently prepared, the dust suppression efficiency can be effectively improved, and the dust suppression cost can be effectively reduced.

The invention relates to the technical field of high polymer materials, in particular to a color-spun superfine sea-island imitation sheepskin fabric and a production method thereof. The color-spun superfine sea-island imitation sheepskin fabric comprises the following raw materials in parts by weight: color-spun superfine sea-island fibers, conventional polyester filaments and a functional additive, the color spinning superfine sea-island fiber is formed by blending alkali-resistant color master batches and a sea-island base material, the mass ratio of an island component to a sea component in the sea-island base material is (5-7): (3-5), and raw materials of the island component and the sea component are polyethylene terephthalate and polyethylene respectively; the functional additive comprises an alkali-resistant stabilizer, a dyeing auxiliary agent and a softening agent. According to the invention, sodium citrate and KH-550 in the alkali-resistant stabilizer react at a constant temperature, sodium citrate is dissolved in the process and forms weak interaction with amino of KH-550 to enhance the stability of the system, and ether bonds of polyoxyethylene fatty acid and sulfonic groups of lauryl sodium sulfate in the dyeing auxiliary agent form a synergistic effect to enhance the emulsifying and wrapping capacity to grease and color paste.

The invention discloses a magnetic biochar coupled dynamic membrane synergistic pollution reduction and flora regulation method, which comprises the following steps: adding magnetic biochar into an anaerobic dynamic membrane bioreactor for anaerobic digestion reaction, and adding 1.2-1.5 g of magnetic biochar into each liter of sludge mixed liquid in the anaerobic dynamic membrane bioreactor. The magnetic biochar is composed of a honeycomb-shaped carbon material obtained by pyrolyzing carbon through sodium citrate and Fe3O4 loaded on the surface of the honeycomb-shaped carbon material through a chemical coprecipitation method. According to the method, functional microorganisms such as methane filamentous bacteria and methane rod bacteria can be selectively enriched, the methane yield is increased by 30%-35%, membrane pollution is effectively relieved by improving the sludge floc structure, the service life of the membrane is prolonged, and technical support is provided for low-cost and low-energy-consumption operation of the anaerobic membrane bioreactor.

The invention discloses a preparation method of modified mineral powder for adjusting and controlling the viscosity of concrete. The preparation method of the modified mineral powder comprises the following steps: placing vertical mill mineral powder in a hydrochloric acid solution for ultrasonic treatment and infiltration to obtain activated mineral powder; preparing a sodium alginate solution and a calcium chloride cross-linking solution, adding the activated mineral powder into the sodium alginate solution to obtain a mineral powder suspension, and dropwise adding the mineral powder suspension into the calcium chloride cross-linking solution to react to obtain calcium alginate coated mineral powder; the preparation method comprises the following steps: dissolving sodium citrate, glucolactone and active nano silicon dioxide in water to form precursor sol; and adding the calcium alginate coated mineral powder into the precursor sol to obtain the modified mineral powder. The modified mineral powder prepared by the method disclosed by the invention realizes double effects of morphology regularization and viscosity controllability, and has an adjusting and optimizing effect on the viscosity problem of concrete when being applied to the concrete; and the method improves the activity of the mineral powder and the bonding strength of interfaces of the mineral powder and other components at the same time, so that the compactness and the overall strength of the concrete are improved.

The invention belongs to the technical field of sensor materials and malathion detection, and relates to a nano-silver modified covalent organic framework composite material, a preparation method and application thereof, and an electrochemical aptamer sensor. The preparation method comprises the following steps: S1, dissolving 1, 3, 5-tri (4-aminophenyl) benzene and 2, 5-divinyl-1, 4-phthalaldehyde in acetonitrile, adding a catalyst to carry out condensation reaction, standing, separating, washing and drying to obtain a covalent organic framework material; s2, dispersing the covalent organic framework material and silver nitrate in water, adding sodium citrate after ultrasonic dispersion, and washing and drying to obtain the nano-silver modified covalent organic framework composite material. The nano-silver modified covalent organic framework composite material prepared by the invention shows excellent crystallinity, extremely high porosity and good conductivity, and realizes high-sensitivity, rapid and accurate quantitative analysis of malathion in traditional Chinese medicinal materials.

The invention discloses a hydrophobic anti-icing material based on silver ions and application in a power transmission line, and belongs to the technical field of hydrophobic anti-icing materials, the hydrophobic anti-icing material is prepared from the following raw materials in parts by weight: 80-120 parts of hydroxyl-terminated polydimethylsiloxane, 5-15 parts of tetraethoxysilane, 0.5-3 parts of silver nitrate, 3-10 parts of hydrophobic nano silicon dioxide and 1-5 parts of nano titanium dioxide. The invention discloses an anti-icing and deicing composite material, which is prepared from the following components in parts by weight: 80 to 120 parts of butyl acetate, 0.1 to 0.5 part of dibutyltin dilaurate, 0.5 to 2 parts of sodium citrate and 2 to 5 parts of modified fluorinated phosphotungstic acid imidazole compound, and solves the problems that the existing material is insufficient in photo-thermal utilization rate, poor in stability in a severe environment, insufficient in hydrophobic capacity and difficult to give consideration to anti-icing and deicing effects. According to the scheme, the modified fluorinated phosphotungstic acid imidazole compound and silver ions are introduced to form a synergistic effect, formation of an ice layer is inhibited, melting of the ice layer is promoted, meanwhile, lasting super-hydrophobicity is provided, a durable hydrophobic anti-icing coating based on the silver ions is formed, and the safety of a power grid is guaranteed.

The invention provides application of sodium citrate as a retardant in ion adsorption type rare earth ore seepage leaching, and relates to the technical field of nonferrous metal mining. The application provided by the invention comprises the following steps: in the in-situ ore leaching process of the ion adsorption type rare earth ore, injecting a sodium citrate solution as a retardant in the downward direction of a diversion hole excavated in the rare earth ore and in other areas needing closure and speed control in advance, so as to reduce the seepage rate of the ore leaching agent in the areas and realize regulation and control of the seepage direction. According to the invention, the leaching liquid can be prevented from seeping downwards through the flow guide holes so as to improve the liquid yield, the seepage extraction of a mineral leaching agent on wider areas such as a semi-weathered layer is enhanced, the mineral leaching blind area is effectively reduced, and the leaching efficiency is improved.

The invention discloses an environment-friendly concrete water reducing agent and a preparation method thereof. The water reducing agent is prepared from the following raw materials in parts by weight: 25 to 60 parts of polycarboxylic acid water reducing agent mother liquor, 1.5 to 3 parts of modified early strength agent, 0.1 to 0.6 part of defoaming agent, 6 to 15 parts of water-retaining agent, 1.5 to 3.5 parts of dispersing agent, 0.4 to 1.2 parts of air entraining agent and 3 to 6 parts of retarder. The preparation method comprises the following steps: dissolving lignosulfonate in water, adjusting the pH value to 9-11, adding hydrogen peroxide, carrying out a reaction at 60-65 DEG C for 2-4 h, and carrying out drying so as to obtain modified lignosulfonate; the modified early strength agent is prepared from sodium citrate, nano silicon dioxide and a silane coupling agent according to the weight ratio of (3 to 5): 1: (0.08 to 0.12). The water reducer provided by the invention has the advantages of efficient water reduction, stable delayed coagulation, early strength and coagulation promotion and environmental friendliness.

The invention discloses a polyaluminum chloride-based flocculant as well as a preparation method and application thereof, belongs to the technical field of preparation of flocculants, and aims at solving the technical problem that the flocculation performance and the stability of a flocculant in the prior art need to be further improved. The surface charges of iron-based particles are adjusted through sodium citrate, so that the problem of particle agglomeration is effectively avoided, the coating uniformity of microgel is improved, and the specific surface area of the material is increased; abundant cation sites are introduced into the trimethylamine modified chitosan, so that the electrostatic adsorption capacity of the microgel on anion pollutants is remarkably enhanced, and meanwhile, the electrical property and the stability of a system are improved; the addition of sodium metasilicate further promotes the modified PAC to form a stable Al-O-Si bridging structure, the material is endowed with good three-dimensional skeleton strength and composite interface stability, the three components cooperate with each other and play key roles in particle dispersity, charge construction and skeleton stability, and finally, an efficient, stable and storable composite flocculation system is constructed.

The invention relates to the technical field of preparation of electro-catalysis electrode materials, in particular to a preparation method and application of a SeO2 doped RuO2 electrolyzed water catalyst.The preparation method comprises the steps that S1, ruthenium acetate and selenium dioxide are dissolved in deionized water together and continuously stirred to obtain a mixed solution A, then sodium citrate is dissolved in deionized water, and the PH value is adjusted to be 9 with ammonia water to obtain a solution B; s2, mixing the solution A and the solution B, reacting at 80 DEG C for 12 hours, naturally cooling, ultrasonically washing with deionized water and ethanol in sequence, centrifuging, and drying in a drying oven to obtain a catalyst precursor; and S3, carrying out two-step pyrolysis on the precursor, namely heating to 250 DEG C, annealing for 4 hours, heating to 400 DEG C, annealing and heating for 2 hours, so as to obtain the SeO2-doped RuO2 nanosheet. By introducing a proper amount of SeO2 for doping, the electronic structure of RuO2 is optimized, the adsorption and desorption balance effect of the catalyst on a reaction intermediate is adjusted, the Ru site catalytic performance is improved, and the obtained SeO2-doped RuO2 nanosheet has efficient acidic OER catalytic performance.

The invention discloses a nickel-molybdenum hydrogen evolution electrode and a preparation method and application thereof, and belongs to the technical field of water electrolysis hydrogen production. The preparation method of the nickel-molybdenum hydrogen evolution electrode provided by the invention comprises the following steps: carrying out alkali oil removal and acid activation treatment on a nickel net to obtain a pretreated nickel net; and the pretreated nickel net serves as a cathode, electrodeposition is conducted in electroplating liquid containing nickel sulfate hexahydrate, sodium molybdate dehydrate, sodium citrate dehydrate, boric acid, sodium chloride and an additive, separation and collection are conducted, and the nickel-molybdenum hydrogen evolution electrode is obtained. The overpotential of the prepared hydrogen evolution electrode material can be as low as 44 mV under the current density of 10 mA cm <-2 >, and the overpotential of the hydrogen evolution electrode material can be as low as 118.5 mV under the current density of 100 mA cm <-2 >. And under the current density of 100 mA cm <-2 > and in a 1 mol / L KOH solution at normal temperature, the material can stably run for more than 250 hours, has excellent stability, and has a wide application prospect in the field of hydrogen production by electrolysis of water.

The invention belongs to the technical field of metal material plating, and particularly relates to a connector surface composite coating and a preparation process thereof, and the preparation process comprises the following steps: adding modified carbon black and sodium molybdate into deionized water, stirring to obtain a corrosion inhibition material, and mixing with modified zinc oxide to obtain a mixed filler; the preparation method comprises the following steps: adding dibutyltin dilaurate and toluene diisocynate into polypropylene glycol to obtain a core material; adding hexadecyl trimethyl ammonium bromide and the mixed filler into a 93-96 wt% ethanol solution, adding the core material for emulsification, adding an isophorone diisocyanate solution, and stirring to obtain a microcapsule; nickel sulfate hexahydrate, nickel chloride hexahydrate, sodium citrate, boric acid, a surface dispersant, a sodium hydroxide solution and microcapsules are added into deionized water to be mixed, and an electroplating solution is obtained; and putting the connector into the electroplating solution for electroplating to obtain the composite coating. The expansion of pitting corrosion can be inhibited, and the long-term use reliability and stability of the connector in a marine environment are improved.

The invention discloses a modified hollow cadmium sulfide photocatalytic reducing agent and a preparation method and application thereof, and belongs to the technical field of wastewater treatment.The preparation method comprises the steps that silicon dioxide microspheres and deionized water are mixed to prepare suspension liquid, a sodium citrate aqueous solution, a cadmium chloride aqueous solution, ammonia water and a thiourea aqueous solution are added into the suspension liquid, the mixture is stirred to be uniform and then heated for a reaction, and the modified hollow cadmium sulfide photocatalytic reducing agent is obtained; preparing to obtain a silicon dioxide and cadmium sulfide composite material; immersing the silicon dioxide and cadmium sulfide composite material into a sodium hydroxide solution, heating and etching to obtain nano hollow cadmium sulfide; the preparation method comprises the following steps: immersing nano hollow cadmium sulfide into a 1-octadecene solution, carrying out heating reaction, and carrying out alcohol washing and drying to obtain the modified hollow cadmium sulfide photocatalytic reducing agent. The modified hollow cadmium sulfide composite photocatalyst is synthesized through a silicon dioxide template method and a thermal decomposition method, and high-concentration methyl orange industrial wastewater can be efficiently degraded under the condition of visible light irradiation.

The invention provides a preparation method of a nano-gold star material, which comprises the following steps: stirring and heating a chloroauric acid solution, adding a sodium citrate solution, continuously stirring and heating, when the color of the solution is changed from colorless to dark red, adjusting the rotating speed to 600rpm, turning off a heating device, cooling at room temperature, filtering the reacted solution by using a water-based filter membrane, and drying to obtain the nano-gold star material. Removing impurities to obtain a gold seed solution; characterizing the gold seed solution and the gold seed particles; the preparation method comprises the following steps: adding a 4-hydroxyethyl piperazine ethanesulfonic acid solution, a gold seed solution, a hydroxylamine solution and a magnetic rotor into deionized water, dropwise adding a 22.5 mM chloroauric acid solution, adjusting the rotating speed to 700rpm, and reacting for 15 minutes at room temperature to obtain the nano gold star material. The prepared nano gold star material is uniform, stable, adjustable in particle size and high in dispersity, the material is in a three-dimensional multi-branch shape, and the diameter of the material is adjustable within 50-200 nm.

The invention provides silver powder and a preparation method thereof, and belongs to the technical field of metal powder preparation. According to the method, the silver powder microstructure control agent and the small-particle silver powder oriented growth inducer are used in a combined mode, the pH value, the temperature and the adding mode of the reaction solution are cooperatively controlled, and the large-flake silver powder with the small-particle thick-flake silver powder growing in an oriented arrangement mode is obtained. An oxidizing agent solution and a reducing agent solution are added into a base solution at the same time, sodium citrate in the base solution controls generation of small-particle thick-sheet-shaped silver powder, an alkaline inducer can promote oriented growth of silver powder particles while adjusting the pH of the solution, then target silver powder is prepared, and on the other hand, in an alkaline reaction system, the prepared silver powder is high in sedimentation rate and high in stability. The solid-liquid separation effect is good, and the waste liquid treatment cost is low. Compared with dendritic or traditional single flaky silver powder, the flaky silver powder prepared through the method has the advantages that the sintering shrinkage rate of powder slurry is low, the contact effect between silver powder particles is better, the sintering activity and conductivity are good, and the market application prospect is good.

The invention relates to the technical field of oil gas well tracing monitoring, in particular to a preparation method and application of an oil gas well tracing monitoring magnetic nano tracer agent. Comprising the following steps: dissolving FeCl3, FeSO4. 7H2O and doped metal salt in water, and introducing N2 to remove oxygen; the initial reaction temperature is 85 DEG C, rapidly adding ammonia water, reacting to generate Fe3O4 nanoparticles, doping elements, reducing the temperature to 60 DEG C, and firstly adding sodium citrate for primary carboxyl modification; adding a mixed solution of oleic acid and PEG twice; the pH value of the reaction system is controlled to be 9.5-10.5 in the whole process. The method has the beneficial effects that the doping amount is remarkably increased, the doping amount of doped elements is multiple times of that in the prior art, and the detection sensitivity is remarkably improved; in addition, oleic acid / PEG composite modification is adopted instead of single citric acid modification, so that the tracer is kept stably dispersed in high-salinity formation water; the superparamagnetism facilitates magnetic separation and recovery, and is suitable for harsh formation conditions.

The invention relates to the technical field of solar cell manufacturing, provides a pyramid suede structure suitable for a perovskite crystalline silicon laminated cell and a preparation method of the pyramid suede structure, and solves the problems that an existing suede structure is low in light capture efficiency and poor in surface morphology consistency. The method comprises the following steps: selecting a (100) crystal orientation monocrystalline silicon wafer for cleaning treatment; preparing a down solution from a plurality of components including boron-containing ammonium hydroxide, sodium hydroxide, sodium silicate, isopropanol, polyethylene glycol, sodium citrate and the like; uniform nucleation points are formed through chemical pre-etching and beta-cyclodextrin surface modification; wherein the boron-containing ammonium hydroxide is prepared by reacting boric acid with ammonia gas under an alkaline condition. The prepared pyramid suede structure is composed of tetrahedral pyramid units which are regularly arranged. The method is stable in process and good in reproducibility, and the prepared textured structure has excellent light capture efficiency and surface consistency and is suitable for manufacturing efficient perovskite crystalline silicon laminated cells.

The invention belongs to the technical field of laundry detergents, and particularly relates to an HPV sterilization underwear laundry detergent containing lysozyme-antibacterial protease and a preparation process. The HPV sterilization underwear laundry detergent containing lysozyme-antibacterial protease comprises the following components in percentage by mass: 12%-18% of modified alkyl glycoside, 4%-6% of an auxiliary surfactant, 1.5%-3% of a lysozyme-antibacterial protease compound, 0.8%-1.5% of double-chain quaternary ammonium salt, 2%-3% of sodium citrate, 1%-2% of glycerol, 1%-1.5% of citric acid, 0.1%-0.2% of a preservative, 0.1%-0.3% of essence and the balance of deionized water. The laundry detergent disclosed by the invention is mild in effect, short in decontamination time and good in decontamination effect, especially has an excellent decontamination effect on protein and sebum, and is especially suitable for decontaminating underwear.

The present invention discloses an environmentally friendly water tank fountain solution and a preparation method thereof, relating to the technical field of fountain solution preparation. The fountain solution comprises, by mass percentage, 3% to 10% ethylene glycol monobutyl ether, 0.5% to 3% ethylene glycol tert-butyl ether, 0.8% to 5% 1,2-propylene glycol, 0.1% to 0.5% sodium citrate, 10% to 91% purified water, and 0.1% to 0.5% defoaming agent. The environmentally friendly water tank fountain solution of the present invention has simple components, high stability, and is environmentally friendly. It also has the characteristics of low surface tension, good wettability, good antibacterial properties, and zero alcohol, thereby ensuring printing effects and printing quality.

The invention discloses a low-stress bleeding processing method for channel catfish. The method comprises the following steps: an acid-base control step, in which the channel catfish is placed in water containing sodium citrate to control the acid-base in the body, thereby increasing the alkali reserve in the body of the fish; a combined light anesthesia step, in which the channel catfish is lightly anesthetized through the combined action of low temperature and a chemical anesthetic, so that the fish exhibits a state in which the gill cover of the fish opens and closes weakly and slowly, and the fish body rolls over and loses balance; a fish body puncture step, in which the blood circulation of the channel catfish is destroyed by a manual or mechanical method, thereby creating a bleeding wound on the fish body; a metabolic bleeding step, in which the fish body is placed in a bleeding tank that has been aerated and oxygenated in advance to saturated dissolved oxygen, for bleeding, while maintaining a certain dissolved oxygen concentration; and an ultrasonic blood purification step, in which the fish body is placed in a bleeding tank equipped with an ultrasonic device for bleeding, thereby accelerating the discharge of residual blood in the body.

The invention provides a hinokitiol-dihydromyricetin composite nano condensation body and a preparation method and application thereof, and the composite nano condensation body comprises the following components in 100 mL of a suspension: 0.3 to 0.5 g of hinokitiol, 0.5 to 0.8 g of dihydromyricetin, 1.0 to 1.5 g of chitosan, 0.5 to 0.8 g of sodium alginate, 2.0 to 3.0 mL of polyethylene glycol, 0.3 to 0.5 mL of Tween, 1.0 to 1.5 mL of glycerol, and the balance of a citric acid-sodium citrate buffer solution. The invention provides a hinokitiol-dihydromyricetin composite nano-coacervate which can synchronously solve the problems of synergistic bacteriostasis, membrane rupture and antibiosis, anti-inflammatory repair, application defects of active ingredients and the like of common animal skin antibacterial drugs. The preparation method of the composite nano coacervate is simple, large-scale production is easy, complex equipment is not needed, it can be ensured that active ingredients of a finished product are evenly distributed, the composite nano coacervate can penetrate through the skin cuticle and can also penetrate through a bacterial biofilm, and the synergistic effect is fully achieved.

The application discloses a gastric cancer immunotherapy efficacy prediction biopsy sample MHC-II expression kit and belongs to the field of kit preparation, and particularly relates to a preparation method of an antigen repair solution, which is prepared by mixing citric acid, sodium citrate, an additive and pure water, and the additive comprises a glucose amido polyether derivative and a propyl gallate / calcium ion complex. The gastric cancer immunotherapy efficacy prediction biopsy sample MHC-II expression kit is composed of the antigen repair solution; the kit comprises the following components in parts by mass: 20-30 parts of a dewaxing solution, 10-20 parts of the antigen repair solution, 2-10 parts of sheep serum, 10-20 parts of an enhancing solution, 15-25 parts of an antibody, 5-15 parts of a staining solution and 10-20 parts of a mounting agent. The gastric cancer immunotherapy efficacy prediction biopsy sample MHC-II expression kit provided by the application has high sensitivity and high specificity.

The invention relates to the technical field of detergents, in particular to a dishwashing detergent for a descaling dishwasher and a preparation method of the dishwashing detergent, and the dishwashing detergent comprises non-fibrous components such as ethylene diamine tetraacetic acid, sodium hydroxide, sodium citrate, sodium polyacrylate, fatty alcohol-polyoxyethylene ether AEO-3 and the like. By optimizing the formula design, the problems of alkali scale and water scale residues are solved, phosphate is not contained, the efficient descaling capacity is achieved, and meanwhile the environmental protection performance is improved.

The application discloses an injectable self-healing self-assembling protamine extract hydrogel and a preparation method and application thereof, and belongs to the technical field of hydrogel materials and wound repair. The hydrogel is prepared by mixing protamine extract and a crosslinking agent solution; the crosslinking agent solution comprises a salt solution with a final concentration of 0.01-4.5 M, 0.01-0.1 M sodium citrate and 0.01-0.1 M sodium bicarbonate; the protamine extract is mixed with the crosslinking agent solution at different proportions (1%-50%, w / v%) and is stirred at 20-35 DEG C until complete gelation; and a series of hydrogels can be prepared after multiple freeze-thaw cycles. The hydrogel prepared by the application has good self-healing, injectable characteristics, blood compatibility and tissue regeneration capacity, and has a wide application prospect.