138results about How to "High relaxation rate" patented technology

Polymerized liposome particles which are linked to a targeting agent and may also be linked to a contrast enhancement agent and/or linked to or encapsulating a treatment agent. The targeting imaging enhancement polymerized liposome particles interact with biological targets holding the image enhancement agent to specific sites providing in vitro and in vivo study by magnetic resonance, radioactive, x-ray or optical imaging of the expression of molecules in cells and tissues during disease and pathology. Targeting polymerized liposomes may be linked to or encapsulate a treatment agent, such as, proteins, drugs or hormones for directed delivery to specific biological sites for treatment.

Disclosed herein are nanostructures comprising a polymeric framework comprising at least five geminal bisphosphonate groups, wherein the geminal bisphosphonate groups independently of each other are incorporated as —R³R⁴C(P═O(OR¹)(OR²))₂, wherein R¹ and R² are independently selected from the group consisting of a negative charge, H, alkyl and aryl, and wherein at least one of R³ and R⁴ is a group connected to the polymeric framework with the proviso that when only one of R³ and R⁴ is such a connected group, the other of R³ and R⁴ is either a group being able to connect to the polymeric framework, or the residue of such a group, or selected from the group consisting of H, OH, OR⁵ and R⁵, wherein R⁵ is a lower alkyl. The polymeric framework may comprise manganese ions. Disclosed are also methods for producing such manganese containing nanostructures, compositions comprising such manganese containing nanostructures and use of such manganese containing nanostructures, i.a. as MRI contrasting agents.

The invention relates to a preparation method of HPEI (hyperbranched polyethyleneimine)-encapsulated ferroferric oxide magnetic nano particles, comprising the steps of: adding Fe source to ultrapure water, then adding NH3.H2O, stirring in air, adding HPEI, and reacting at 134-140 DEG C for 3h, wherein the mass ratio of Fe source to HPEI is 1-5 :1; cooling, and carrying out washing and magnetic separation on precipitate to obtain HPEI-encapsulated ferroferric oxide nano particles Fe3O4/HPEI; and then carrying out different surface modification on the Fe3O4/HPEI nano particles, such as polyethylene glycolation (PEGlation), acetylation and carboxylation, so as to increase biocompatibility of the nano particles to be used for MRI (magnetic resonance imaging) diagnosis. The process is simple, the reaction conditions are mild and operation and separation are easy; and the prepared iron oxide magnetic nano particles have excellent biocompatibility and T2 relaxation effect, and have potential application value in the field of MRI imaging diagnosis.

The invention relates to a polysaccharide macromolecular paramagnetic metal complex and a synthesis method and an application thereof. Polysaccharide macromolecules are used as carriers, and a ligand compound, the side chains of which contain open-chain or annular polyamino polycarboxylic compounds, acts with paramagnetic metal ions to form the paramagnetic metal complex, wherein the paramagnetic metal ions are positive divalent or trivalent ions of metal elements, the atomic numbers of which are 21-29, 42 and 44 or 57-71. The polysaccharide macromolecular paramagnetic metal complex can be used as a magnetic resonance imaging contrast agent for lymphatic organs, lymphatic vessels, lymphatic systems and cardiovascular systems of people or other mammals.

The invention synthesizes a type of liver targeted, biodegradable high molecular MRI contrast medium with alpha,beta- poly [(2-aminoethyl) - L-asparagine] as fundamental chain, galactose as target gene, diethylenetriamine pentaacetic acid or L-tyrosine methyl ester derivatives as Gd3+ ligand. The contrast agent is less toxic, biocompatible, and has good relaxation rate than low molecular business contrast medium. Animal experiments showed that in liver organizations its enhancing effect is three times better than commercial and low molecular contrast medium (gadolinium sprayed acid cyelophosphate); the residence time in liver is more than 24 hours, which is 18 hours longer than low molecular business contrast medium. The feature is that Zeta potential for the contrast medium is negative and less than -10mV. We can achieve the MRI visible, the liver targeted, core-shell nanogene vector system with it as a gene vector system shell structure.

The invention relates to a nanometer magnetic resonance contrast material, a nanometer magnetic resonance contrast agent and a preparation method of the nanometer magnetic resonance contrast agent. The nanometer magnetic resonance contrast material comprises a core having the chemical formula of A3x[GdyFeIII1-y](4-x)[FeII(CN)6]3.nH2O, and a casing which is composed of auxiliary materials and coats the core. In the chemical formula, A represents Li<+>, Na<+>, K<+> or NH4<+>, x is greater than 0 and less than or equal to 1, y is in a range of 0-1, and n is in a range of 1-16. The nanometer magnetic resonance contrast agent prepared from the nanometer magnetic resonance contrast material has stable properties, is decomposed and ionized difficultly, has low toxicity and is suitable for injection and oral administration.

The invention provides a preparation method of a targeted superparamagnetic nano-probe. The method includes the steps of: 1. preparing ferroferric oxide nano-particles with a particle size of 10-20nm in an oil phase by high temperature thermal decomposition; 2. synthesizing an amphiphilic polymer; 3. conducting aqueous phase transformation of the ferroferric oxide nano-particles obtained by step 1 through the amphiphilic polymer obtained by step 2 so as to obtain water-soluble superparamagnetic nano-particles; and 4. under the coupling effect of EDC/NHS, subjecting the water-soluble superparamagnetic nano-particles obtained by step 3 to covalent coupling with a target molecule, thus obtaining the targeted superparamagnetic nano-probe. The invention also provides an application method of the probe. Under the action of a separation column and an applied magnetic field, the probe can specifically and effectively capture and separate target cells. Compared with the prior art, the probe prepared by the method provided by the invention has the advantages of uniform particle size, high T2 relaxation rate, good stability, high pH value, high salt tolerance, and good target cell separation effect.

The invention relates to a multi-mode targeted probe for early hepatic fibrosis diagnosis and a preparation method thereof. The multi-mode targeted probe for the early hepatic fibrosis diagnosis is characterized by comprising a vector, and the vector is connected with a fluorescence group and a magnetic resonance imaging group and can be connected with a targeted compound group, which can be combined with a receptor on the surface of a hepatic stellate cell in hepatic fibrosis/cirrhosis, through a bridging group. The preparation method comprises the steps of: connecting the targeted compound group, which can be combined with the receptor on the surface of the hepatic stellate cell in the hepatic fibrosis/cirrhosis, with the bridging group, and sequentially connecting the targeted compoundgroup, the fluorescence group and the magnetic resonance imaging group on the vector. The targeted probe has higher sensitivity when being used for diagnosing and evaluating the fibrosis diagnosis.

The invention provides a contrast agent as well as a preparation method and an application thereof. The contrast agent is formed by taking Mn3[Co(CN)6]2 nanometer particles as an inner core and a silica coating layer as a casing. The contrast agent which is high in relaxation rate, low in toxicity and high in fluorescent quantum yield can be used for T1-T2 dual-mode magnetic resonance imaging, magnetic resonance angiography, multiband bioluminescence imaging and dual-photon bioluminescence imaging.

The invention discloses a bismuth sulfide/gadolinium oxide-entrapping ultrafine protein composite nanoparticle with a near-infrared photothermal effect and a multi-mode imaging function and a preparation method and application thereof. By formulation screening and process optimization, the bismuth sulfide/gadolinium oxide albumin ultrafine composite nanoparticle with the near-infrared photothermaleffect and the multi-mode imaging function is prepared in one step. The composite nanoparticle has good physicochemical stability, photostability, biocompatibility and tumor-targeting property, can remarkably enhance near-infrared fluorescence, photoacoustic, magnetic resonance, CT (computed tomography) and thermal signals at a tumor site, and realizes multi-mode-complemented tumor diagnosis, andmoreover, the composite nanoparticle can eliminate tumors under the excitation of near-infrared light; in particular, because the grain size of the nanoparticle is less than 5.5nm, the nanoparticle can be eliminated by the kidneys, and toxic and side effects are minor; a tumor can be diagnosed and treated at the same time, and the composite nanoparticle has a great potential in realizing accuratetumor diagnosis and treatment integration.

The invention discloses a precious metal/paramagnetic metal composite nanoparticle with a core-shell structure and application thereof. A preparation method for the precious metal/paramagnetic metal composite nanoparticle comprises the following steps: (a) preparing a precious metal nanoparticle with a particle size of 5 to 50 nm, wherein the precious metal is gold or silver; (b) taking a compound A containing a mercapto group and an amino group, allowing the precious metal nanoparticle to react with the compound A so as to obtain a product a and then allowing the product a to react with methyl acrylate so as to obtain a product b; (c) taking a compound C containing an azido group and an amino group and reacting the product obtained in the step (b) with the compound C so as to obtain an azido-surface-modified precious metal nanoparticle; (d) subjecting the azido-surface-modified precious metal nanoparticle prepared in the step (c) and a micromolecular ligand containing an alkynyl group to the Clicker reaction so as to obtain a product d; and (e) reacting the product d with a chloride of a paramagnetic metal so as to obtain the composite nanoparticle. The precious metal/paramagnetic metal composite nanoparticle can be used as an MRI and CT enhancement contrast agent.

The invention relates to the technical field of preparing a contrast agent by utilizing a hyperbranched polymer. A preparing method of the hyperbranched polymer includes reacting a lysine salt and caustic alkali in a mole ratio of 100:80-90 at 140-160 DEG C for 40-50 h in a protective atmosphere; mixing a hyperbranched polylysine skeleton and diethylenetriaminepentaacetic acid according to a mass ratio of at least 1:5 in water; reacting the mixture at room temperature for 6-8 h; mixing the first hyperbranched polylysine molecule and a gadolinium compound in water; reacting the mixture at 37-42 DEG C for 6-12 h, with the mass ratio of the first hyperbranched polylysine molecule to the gadolinium compound being 1:1-1:2; and mixing and reacting the second hyperbranched polylysine molecule and folic acid in a carbonate the pH value of which is 8-10 for 5-12 h. The hyperbranched polylysine contrast agent has good biocompatibility, low toxicity and a high relaxation rate and can be metabolized in vivo.

The invention relates to a magnetic nanometer material used for imaging and photothermal therapy and a preparation method and application of the magnetic nanometer material. The magnetic nanometer material used for imaging and photothermal therapy refers to nanometer iron oxide particles coated with melanin. The magnetic nanometer material is non-toxic, high in biocompatibility and capable of imaging. According to the preparation method, a one-step coprecipitation method is adopted to composite water-soluble Fe3O4@mel NCs. The magnetic nanometer material has the advantages of mildness and simplicity.

The invention discloses a method for preparing a paramagnetic polyphosphazene nanotube magnetic resonance imaging contrast agent. The method is characterized by firstly taking hexachlorocyclotriphosphazenes and 4,4'-dihydroxydiphenylsulfone as comonomers and triethylamine as an acid binding agent to carry out polymerization under room temperature to prepare the polyphosphazene nanotube and obtaining a polyphosphazene nanotube ligand after purifying the polyphosphazene nanotube; and then reacting the ligand with paramagnetic metal inorganic salt, finally centrifuging the reactant, washing the product three times respectively with water and absolute ethyl alcohol, thus obtaining the solid powder of the magnetic resonance imaging contrast agent after drying. The method is strong in operability, wide in application range, simple in equipment requirement, low in cost and high in yield and can be used for mass production in industry application. The magnetic resonance imaging contrast agent of the invention is a novel polymer nanotube magnetic resonance imaging contrast agent. The relaxation rate of the magnetic resonance imaging contrast agent is as high as 42.88-237.83mmol/L*s and is 12-65 times that of the commercial magnetic resonance imaging contrast agents Gd-DTPA (with relaxation rate of 3.64mmol/ L*s).

The invention provides a diamino polyethylene glycol modified paramagnetic metal complex magnetic resonance imaging contrast agent, and aims at solving the problems of low relaxation rate, high toxicity and large dosage of a contrast agent in the prior art. The contrast agent is prepared by the following steps: reacting 1,4,7,10-tetrazacyclododecane-1,4,7,10-tetraacetic acid compound with diaminopolyethylene glycol with different molecular weights; preparing a macromolecular ligand through an amidation reaction; and forming a paramagnetic metal complex with paramagnetic metal elements Sc, Ti, V, Mn, Cu, Mo, Ru, Fe or positive trivalent or divalent ion of lanthanide rare-earth elements. The contrast agent has the advantages of high stability, water solubility, relaxation rate and small dosage.

The invention discloses a tumor-targeted magnetic resonance imaging and fluorescent supermolecular imaging contrast agent and preparation and application thereof. The tumor-targeted magnetic resonanceimaging and fluorescent supermolecular imaging contrast agent is characterized in that a water-soluble macromolecular skeleton material is modified by molecules with a specific binding function, andis self-assembled with corresponding ligand molecules to form supermolecular nanoparticles under the mutual action of a host and a guest, and the ligand molecules are modified with a paramagnetism material with an imaging function and a near-infrared fluorescent dye. The tumor-targeted magnetic resonance imaging and fluorescent supermolecular imaging contrast agent has the advantages that the tumor cells of a receptor with high expression specificity can be targeted; the magnetic resonance imaging and fluorescent double imaging functions are realized; the contrast agent can be used for the in-operation fluorescent guidance positioning and the post-operation magnetic resonance monitoring in the tumor cutting operation.

The invention relates to a HA-targeted layered doubled hydroxide-ultrafine iron nano material and preparation and applications thereof. The preparation is as follows: synthesizing an LDH-Fe3O4 nano composite material by a coprecipitation method; covalently bonding activated hyaluronic acid on the surface of an LDH laminate through a silane coupling agent; and finally, carrying out surface loadingof an anticancer drug. The nano-material particles are uniformly distributed, and can enhance the MR imaging effect of the tumor part in an animal body. The LDH-Fe3O4-HA NPs is used as a carrier of ananticancer drug doxorubicin DOX, not only has sensitive pH response and release characteristics, but also can carry out specific recognition on tumor cells expressed by CD44 receptors so as to achieve the idealization effect of efficiently inhibiting tumors.

The invention belongs to the technical field of biological medicine, and particular relates to a magnetism polymer nanometer microsphere material capable of degrading ring structure , as well as a preparation method and application of the magnetism polymer nanometer microsphere material. The backflow precipitation polymerization technology is adopted in the invention, Fe3O4 magnetic clusters stabilized by polyglutamate or agarose are used as cores; a polymer shell which is not crosslinked is firstly wrapped on the exterior, a new polymer shell with crosslinked disulfide bond is secondly wrapped, alcohol or water is used for etching the polymer not crosslinked, and the degradable magnetism polymer microcapsules with crosslinked disulfide bond are obtained. The method provided by the invention is quick and simple in post-treatment, requires no etching using a strong acid or a strong alkali, and is safe and efficient; the prepared material can is very good monodispersity, and can be applied to the fields of ultrasound and magnetic resonance image formation contrast agent and medicine carrier.

The invention provides a double-layered core-shell structure molecular carrier. The double-layered core-shell structure molecular carrier is characterized in that a modified sodium gadolinium tungstate nano-rod NaxGdWO3 is used as an inner core and the outer surface of the inner core is coated with mesoporous silicon dioxide; a double-layered structure is WCMSNs. The molecular carrier not only canbe used for carrying out magnetic resonance imaging and photo-thermally burning tumor cells to die, but also can load various medicines or target molecules, so that drug treatment or targeting localization is carried out on the basis of the magnetic resonance imaging and photo-thermal treatment.