Preparation method of gadolinium oxide magnetic resonance imaging nano probe with good biocompatibility

A biocompatibility, magnetic resonance imaging technology, which is applied to preparations and pharmaceutical formulations for in vivo experiments, can solve the problem that gadolinium contrast agents cannot meet special imaging needs, and can reduce in vivo immune responses and preparation conditions. Simple and convenient for large-scale production

Inactive Publication Date: 2015-07-22
TIANJIN MEDICAL UNIV
View PDF0 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the invention is in order to solve the clinically widely used low T 1 The gadolinium-based contrast agent with relaxation efficiency cannot meet the special imaging requirements at high magnetic field strength 3T, and the development of gadolinium-based T 1 Magnetic resonance molecular probe, providing a preparation method of gadolinium oxide magnetic resonance imaging nanoprobe with high relaxation rate and good biocompatibility

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of gadolinium oxide magnetic resonance imaging nano probe with good biocompatibility
  • Preparation method of gadolinium oxide magnetic resonance imaging nano probe with good biocompatibility
  • Preparation method of gadolinium oxide magnetic resonance imaging nano probe with good biocompatibility

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1) In a beaker, dissolve 0.2g of enzymatically cleaved hyaluronic acid sodium salt with a molecular weight of 5000 in 10ml of water, and stir until completely dissolved. Add 1mL of 0.1M Gd(NO 3 ) 3 ·6H 2 O aqueous solution, in which hyaluronic acid sodium salt and Gd(NO 3 ) 3 ·6H 2 The mass ratio of O was 40:9. After 5 min, NaOH aqueous solution was added to adjust the pH to 10. After continuing to stir at room temperature for 1 h, a colorless liquid was obtained;

[0021] 2) Put the obtained hyaluronic acid gadolinium oxide nanomaterial into a dialysis bag (molecular weight cut-off is 8000-14000Da), and purify. The dialysis time is 24 hours, and the water is changed every 4 hours. After dialysis, a total volume of 50 mL of purified nanoprobe solution was obtained;

[0022] 3) Obtain a flocculent white solid product by freeze-drying, which is a gadolinium oxide magnetic resonance nanoprobe;

[0023] Get 20mg of the magnetic resonance imaging probe solid prepared...

Embodiment 2

[0025] A method for preparing a gadolinium oxide magnetic resonance imaging nanoprobe with good biocompatibility, the steps and methods are basically the same as in Example 1, except that the sodium hyaluronic acid and Gd(NO 3 ) 3 ·6H 2 O mass ratio is 200:9.

[0026] Get 20mg of the magnetic resonance imaging probe solid prepared by this embodiment and carry out high power transmission electron microscope characterization, T 1 Relaxation rate and T 1 Weighted imaging and in vitro cytotoxicity test MTT, the test results are similar to Example 1.

Embodiment 3

[0028] A method for preparing a gadolinium oxide magnetic resonance imaging nanoprobe with good biocompatibility, the steps and methods are basically the same as in Example 1, except that the sodium hyaluronic acid and Gd(NO 3 ) 3 ·6H 2 The O mass ratio is 80:9.

[0029] Get 20mg of the magnetic resonance imaging probe solid prepared by this embodiment and carry out high power transmission electron microscope characterization, T 1 Relaxation rate and T 1 Weighted imaging and in vitro cytotoxicity test MTT, the test results are similar to Example 1.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
sizeaaaaaaaaaa
Login to view more

Abstract

The invention relates to a preparation method of a gadolinium oxide magnetic resonance imaging nano probe with good biocompatibility. The preparation method of the gadolinium oxide magnetic resonance imaging nano probe with good biocompatibility comprises the following steps: 1) in a breaker, dissolving biomacromolecules in water, and stirring until the biomacromolecules are completely dissolved; adding a Gd(NO3)3.6H2O aqueous solution into the biomacromolecule solution, uniformly stirring, adding a NaOH aqueous solution, then regulating pH to be 10, and continuously stirring for 1 hour at room temperature, so that a colourless liquid is obtained; 2) purifying the obtained impurity-containing gadolinium oxide nano probe, carrying out dialysis for 24 hours, and changing water every other 4 hours, so that the purified nano probe is obtained after dialysis is carried out; and 3) carrying out free-drying, so that a flocculent white solid product is obtained. The preparation method of the gadolinium oxide magnetic resonance imaging nano probe with good biocompatibility has the advantages of simplicity, simple preparation conditions, environment-friendly raw materials, good water solubility, high T1 relaxation rate and low potential biological toxicity, is convenient for large-scale production and has large development potential and great application value in the field of medical imaging.

Description

technical field [0001] The invention relates to the field of preparation of magnetic resonance imaging probes, in particular to a preparation method of gadolinium oxide magnetic resonance imaging nano-probes with good biocompatibility. Background technique [0002] Compared with other imaging methods such as optical imaging, X-ray imaging, computed tomography (CT) and positron emission computed tomography (PET-CT), magnetic resonance imaging (MRI) has no radiation damage and high spatial Features such as resolution play an extremely important role in the clinical diagnosis of various diseases. When imaging some lesions with low tissue resolution, in order to improve the sensitivity and accuracy of diagnosis, it is necessary to use contrast agent to assist imaging. Magnetic resonance contrast agents can be divided into positive contrast agents (such as gadolinium and manganese complexes or nanomaterials) and negative contrast agents (such as superparamagnetic ferric oxide na...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/14A61K49/12
Inventor 于春水王浩宇孙少凯
Owner TIANJIN MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap