Preparation method of gadolinium oxide magnetic resonance imaging nano probe with good biocompatibility
A biocompatibility, magnetic resonance imaging technology, which is applied to preparations and pharmaceutical formulations for in vivo experiments, can solve the problem that gadolinium contrast agents cannot meet special imaging needs, and can reduce in vivo immune responses and preparation conditions. Simple and convenient for large-scale production
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Embodiment 1
[0020] 1) In a beaker, dissolve 0.2g of enzymatically cleaved hyaluronic acid sodium salt with a molecular weight of 5000 in 10ml of water, and stir until completely dissolved. Add 1mL of 0.1M Gd(NO 3 ) 3 ·6H 2 O aqueous solution, in which hyaluronic acid sodium salt and Gd(NO 3 ) 3 ·6H 2 The mass ratio of O was 40:9. After 5 min, NaOH aqueous solution was added to adjust the pH to 10. After continuing to stir at room temperature for 1 h, a colorless liquid was obtained;
[0021] 2) Put the obtained hyaluronic acid gadolinium oxide nanomaterial into a dialysis bag (molecular weight cut-off is 8000-14000Da), and purify. The dialysis time is 24 hours, and the water is changed every 4 hours. After dialysis, a total volume of 50 mL of purified nanoprobe solution was obtained;
[0022] 3) Obtain a flocculent white solid product by freeze-drying, which is a gadolinium oxide magnetic resonance nanoprobe;
[0023] Get 20mg of the magnetic resonance imaging probe solid prepared...
Embodiment 2
[0025] A method for preparing a gadolinium oxide magnetic resonance imaging nanoprobe with good biocompatibility, the steps and methods are basically the same as in Example 1, except that the sodium hyaluronic acid and Gd(NO 3 ) 3 ·6H 2 O mass ratio is 200:9.
[0026] Get 20mg of the magnetic resonance imaging probe solid prepared by this embodiment and carry out high power transmission electron microscope characterization, T 1 Relaxation rate and T 1 Weighted imaging and in vitro cytotoxicity test MTT, the test results are similar to Example 1.
Embodiment 3
[0028] A method for preparing a gadolinium oxide magnetic resonance imaging nanoprobe with good biocompatibility, the steps and methods are basically the same as in Example 1, except that the sodium hyaluronic acid and Gd(NO 3 ) 3 ·6H 2 The O mass ratio is 80:9.
[0029] Get 20mg of the magnetic resonance imaging probe solid prepared by this embodiment and carry out high power transmission electron microscope characterization, T 1 Relaxation rate and T 1 Weighted imaging and in vitro cytotoxicity test MTT, the test results are similar to Example 1.
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