Preparation method and application method of targeted superparamagnetic nano-probe

Abstract

The invention provides a preparation method of a targeted superparamagnetic nano-probe. The method includes the steps of: 1. preparing ferroferric oxide nano-particles with a particle size of 10-20nm in an oil phase by high temperature thermal decomposition; 2. synthesizing an amphiphilic polymer; 3. conducting aqueous phase transformation of the ferroferric oxide nano-particles obtained by step 1 through the amphiphilic polymer obtained by step 2 so as to obtain water-soluble superparamagnetic nano-particles; and 4. under the coupling effect of EDC/NHS, subjecting the water-soluble superparamagnetic nano-particles obtained by step 3 to covalent coupling with a target molecule, thus obtaining the targeted superparamagnetic nano-probe. The invention also provides an application method of the probe. Under the action of a separation column and an applied magnetic field, the probe can specifically and effectively capture and separate target cells. Compared with the prior art, the probe prepared by the method provided by the invention has the advantages of uniform particle size, high T2 relaxation rate, good stability, high pH value, high salt tolerance, and good target cell separation effect.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method and an application method of a targeting superparamagnetic nanometer probe. Background technique [0002] Magnetic nanoparticles are a kind of nanomaterials with magnetic responsiveness. Taking advantage of their good biocompatibility, specificity and magnetic anisotropy, magnetic nanoprobes with diverse functions can be prepared through surface modification, so they can be It is widely used in the fields of biological separation and medical detection, and now, it has achieved remarkable results in magnetic imaging, magnetic hyperthermia, bacterial separation, drug delivery, etc. Extensive development and application of magnetic nanomaterials in biomedicine and clinical testing has become a current research hotspot. [0003] Traditional targeted magnetic nanoprobes often use magnetic nanoparticles prepared by hydrothermal method, physical vapor depos...

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Problems solved by technology

[0003] Traditional targeted magnetic nanoprobes often use magnetic nanoparticles prepared by hydrothermal method, physical vapor deposition method, etc. as carriers. Although the preparation process is simple and the cost is low, the obtained magnetic nanoparticles often have uneven particle size distribution. , Poor water solubility, easy to agglomerate, so that the later surface modification brings certain difficulties; in addition, the traditional magnetic nanoparticles are often between 50 and 200nm in diameter, which belong to ferromagnetic or ferrimagnetic nanoparticles, and respond to external magnetic fields. If it is too strong, when the target cells are captured and used for collection, the cells may be deformed or even broken due to the effect of an external strong magnetic field, which will greatly affect the sorting rate and sensitivity of the target cells and cause difficulties for later medical applications.

[0004] With the development of science and technology and clinical application, people's requirements for magnetic nanomaterials are getting higher and higher, and the magnetic nanoparticles currently used can no longer meet people's needs.

The main reason is that its preparation process and surface modification effect are not ideal, mainly manifested in: large particles, poor dispersion, weak magnetic anisotropy, uneven particle size distribution, unstable surface modification, etc. Results such as low sensitivity and poor targeting in applications such as separation have brought many problems to later animal experiments and clinical applications

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