The application provides a
biosensor based on exchange
coupling effect and magnetic elastic
coupling effect and a preparation method thereof, and belongs to the field of biosensors. The
biosensor comprises, from bottom to top, a flexible substrate, a silver interdigital
electrode prepared by a magnetron
sputtering process arranged on the surface of the flexible substrate, a PDMS stress transfer layer arranged on the interdigital
electrode, a magnetic sensitive unit composed of magnetostrictive
layers and soft magnetic
layers alternately stacked, and a gold biological
interface layer arranged on the topmost layer. The application significantly reduces the
interface impedance and optimizes the
signal collection efficiency by introducing the silver interdigital
electrode. The weak
surface stress generated by the combination of biological molecules is efficiently converted into a significant change in
magnetic anisotropy by utilizing the synergistic effect of exchange
coupling and magnetostrictive effect in the magnetic multilayer film. The
biosensor not only solves the problem of
slow response to
surface stress of traditional devices, but also has excellent mechanical flexibility, high
signal-to-
noise ratio and high sensitivity, and is suitable for
label-free detection of complex biological samples.