31results about How to "Increase folic acid content" patented technology

The invention provides a preparation method of a targeted superparamagnetic nano-probe. The method includes the steps of: 1. preparing ferroferric oxide nano-particles with a particle size of 10-20nm in an oil phase by high temperature thermal decomposition; 2. synthesizing an amphiphilic polymer; 3. conducting aqueous phase transformation of the ferroferric oxide nano-particles obtained by step 1 through the amphiphilic polymer obtained by step 2 so as to obtain water-soluble superparamagnetic nano-particles; and 4. under the coupling effect of EDC / NHS, subjecting the water-soluble superparamagnetic nano-particles obtained by step 3 to covalent coupling with a target molecule, thus obtaining the targeted superparamagnetic nano-probe. The invention also provides an application method of the probe. Under the action of a separation column and an applied magnetic field, the probe can specifically and effectively capture and separate target cells. Compared with the prior art, the probe prepared by the method provided by the invention has the advantages of uniform particle size, high T2 relaxation rate, good stability, high pH value, high salt tolerance, and good target cell separation effect.

The invention discloses high-protein fermented poultry and livestock feed and a preparation method thereof. The preparation method comprises the following steps that 1, 20%-40% of fly maggots, 20%-40% of cracked corn and the balance wheat bran are weighed by mass to be put into a container, beer yeast of which the weight accounts for 0.4% to 0.8% of the total weight of the raw materials and water of which the weight accounts for 0.5-2 times of the total weight of the raw materials are added for secondary fermentation, and primary fermentation materials are obtained; 2, 60%-80% of the primary fermentation materials and the balance packing are weighed by mass, beer yeast of which the weight accounts for 0.4% to 0.8% of the weight of the mixed materials and water of which the weight accounts for 0.1-0.5 time of the weight of the mixed materials are added for secondary fermentation, and secondary fermentation materials are obtained; 3, the obtained secondary fermentation materials are sterilized and then granulated, and the feed is obtained. The high-protein fermented poultry and livestock feed prepared through the method is green, environmentally friendly and capable of effectively increasing the poultry and livestock weight and improving the poultry and livestock meat mouthfeel.

The invention provides a method for preparing soybean milk fermented milk by lactobacillus strains capable of producing high-yield folic acid. The method comprises the following steps: inoculating screened strains LZ217 (Lactobacillus sakei LZ217) into fresh soybean milk and fermenting the mixture in a 37-DEG C constant-temperature fermentation box to obtain the soybean milk fermented milk which has rich natural folic acid. The side effect of chemically synthesizing folic acid in a traditional industry is eliminated, and the content of folic acid is remarkably increased when being compared with that of other products; and furthermore, folic acid synthesized by fermenting the fermented soybean milk has reducibility and can be absorbed by human bodies very well, so that folic acid needed by the human bodies can be effectively supplemented, and various types of body discomfort, caused by the fact that folic acid is lacked, are alleviated to a certain extent.

The invention provides a lactic acid bacterium composition which is prepared from streptococcus thermophilus inm25-ST, lactobacillus delbrueckii subsp. bulgaricus inm25-LB and a promoting factor. Thecomposition is used for improving folic acid metabolism capability of the lactobacillus sobrinus LZ217 during fermentation and has the advantages that the production performance is good and stable, and the lactic acid bacterium composition can be put into a factory for mass production. In addition, the obtained fermented product has good taste, texture and flavor, less whey separation in a qualityguarantee period, weak post-acidification and high active folic acid content.

The invention relates to a nutritional congee with millet as a raw material. The objective of the invention is to provide a milk millet congee with high folic acid content and spleen-invigorating and stomach-nourishing functions. A making method for the milk millet congee comprises the following steps: repeating freezing and unfreezing of 30 parts of millet; weighing 30 parts by mass of rice, soaking the rice with water for 30 min and cooking the rice on a big fire for 20 to 25 min; and adding 120 parts by mass of milk, the unfrozen millet and sodium bicarbonate water, carrying out decoction on a small fire after the obtained mixture is boiled, and carrying out continuous stirring until rice grains are completely cooked. According to the theory of traditional Chinese medicine, the milk millet congee provided by the invention has health-preserving efficacy: millet attributes to the spleen and stomach meridians and is capable of invigorating the spleen and nourishing the stomach; and through cooking of combined millet and milk rich in protein, the milk millet congee can protect the health of the stomach and is applicable to people with weakness of the spleen and the stomach.

The invention relates to the field of plant gene engineering, and specifically relates to a method for increasing plant folate content by using a synergistic effect of transferred soybean genes of GTPCHI and ADCS. The gene sequence of GTPCHI synthetic key enzyme of the soybean folate is shown as SEQ ID No. 1; and the gene sequence of ADCS is shown as SEQ ID No. 2. The synergistic effect of the transferred soybean genes of GTPCHI and ADCS can obviously increase the folate content in transgenic plants. Therefore, the method can be used as a method for producing the transgenic plants with high folate content. People can obtain natural folates directly by eating the plants; phenomenon that folate intake is lack can be solved; and adverse side effects brought by long-term taking folate tablets can be overcome.

The invention discloses application of OsAPBP2 protein to promotion of synthesis of plant folic acid. The OsAPBP2 protein provided by the invention is protein shown as a) or b) or c) or d): a) protein with an amino acid sequence shown as a sequence 2; b) fused protein obtained by connecting an end N and / or an end C of the protein shown as the sequence 2 with a label; c) protein which is obtained by carrying out substitution and / or deletion and / or addition of one or more amino acid residues on the amino acid sequence shown as the sequence 2, and has the same function; d) protein which has 75 percent or more than 75 percent of homology with the amino acid sequence shown as the sequence 2 and has the same function as the sequence 2. An experiment proves that the OsAPBP2 protein can be used for identifying and combining with rice folic acid to synthesize a key gene OsADCS promoter to regulate and control the expression of a rice folic acid synthesis key gene and further regulate and control the content of the folic acid in rice, and plays an important role in the synthesis of the plant folic acid.

The invention provides a saline-alkali resistant composition containing a herba houttuyniae extract. The saline-alkali resistant composition is characterized by being prepared from herba houttuyniae. The invention further provides a preparation method of the saline-alkali resistant composition containing the herba houttuyniae extract. The method is characterized by comprising a fermented pulp preparation step, wherein the fermented pulp preparation step comprises the following specific steps: mixing chlamydomonas reinhardtii, a red algae extract, corn meal, rye flour and sheep bone soup serving as raw materials of the composition according to a proportion by parts; decocting the mixture with soft fire for 60-65minutes after boiling; cooling; sealing the mixed liquid, and storing at the temperature of 35-37 DEG C for 10-12 days; ventilating; adding yeast powder in the ventilating process; preserving heat at the temperature of 32-35 DEG C for 15-18 days after finishing ventilation to obtain the fermented pulp. Through application of the saline-alkali resistant composition to cucumbers planted in saline-alkali soil, the folic acid content, vitamin B6 content, vitamin C content and vitamin K content of the cucumbers can be increased.

The invention relates to cheese suitable for pregnant women to eat. The cheese is prepared from the following components in percentage by weight: natural cheese, fat products, solid milk beverages, a stabilizing agent, an emulsifying agent, an antioxidant, emulsifying salt, an acidity adjusting agent, black kerneled rice, purple rice, spinach, raisin, large pheasant eggs, and the balance being water. The cheese which is prepared from the natural cheese, the solid milk raw materials and various auxiliary materials not only maintains the nutrient components of the natural cheese, but also is convenient to transport and store; the black kerneled rice, the purple rice and the raisin in the cheese all have the efficacies of replenishing blood and benefiting qi, and can be used for treating the symptoms of anemia and deficiency of the pregnant women during the period of pregnancy; the spinach in the cheese, which contains rich folic acid, can protect foetuses from being damaged by nervous system deformity, such as spinal division, hydrocephalus, and anencephalia; egg white in the large pheasant eggs contains a small amount of antibacterial peptide, after the cheese is eaten by a postoperative patient, the healing velocity of wounds can be accelerated, and the resistance and the immunity of patients, the pregnant women and infants are improved; after parturients and the pregnant women eat the cheese, the quality of breast milk can be improved, and the intelligence of the infants can also be improved.

The invention discloses a preparation method of a composite polysaccharide gel based on folic acid targeting, and belongs to the technical field of biology macromolecules. The preparation method comprises the following steps of enabling a coupling agent to modify acid polysaccharide, coupling with the folic acid, and performing in-situ releasing, so as to prepare a three-component composite polysaccharide gel with an interpenetration crosslinking network structure and high mechanical strength. The composite polysaccharide gel has the in-vitro drug-release targeting property based on the identification of folic acid receptor at the surface of tumor cell. The preparation method has the advantages that the coupling effect is good, and the conversion rate is high; the composite polysaccharidegel can load various anti-tumor drugs, and the application range of the natural macromolecules is widened.

The invention relates to the field of plant gene engineering, and specially, relates to soybean folate synthesis key enzymes GTPCHI and their genes and use. Amino acid sequences of the two soybean folate synthesis key enzymes GTPCHI are respectively shown in the formula SEQ ID No.1 and the formula SEQ ID No.3. Through the expression of the genes of the soybean folate synthesis key enzymes GTPCHI,folate content of a transgenic plant is obviously improved. Therefore, the soybean folate synthesis key enzymes GTPCHI lay the foundation for plant folate content improvement, enable people to directly get natural folate for daily needs through diet, and are very meaningful to relieve folate intake lack of people.

The invention relates to the technical field of feed, and provides a laying hen feed and a preparation method thereof, wherein the laying hen feed comprises the following components in parts by weight: 15-18 parts of soybean, 15-20 parts of soybean meal, 10-16 parts of grease, 8-9 parts of bran, 10-14 parts of wheat bran, 5-8 of fish meal, 3-5 parts of erythrophyll, 2-4 parts of a protein powder, 10-15 parts of alfalfa, 4-6 parts of a premix, and 3-8 parts of a shell powder. The laying hen feed can improve the disease resistant ability of laying hens, reduce the rectocele rate and increase the layering rate, and especially can reduce cholesterols in eggs, deepen egg yolk color, improve egg yolk condition and improve contents of egg selenium, folic acid, unsaturated fatty acids and other nutrients; the feed proportion is simple, the preparation method is easy, and the cost of the feed is reduced.

The invention discloses a breeding method capable of improving egg folic acid content. The method comprises the first step of laying padding which can degrade chicken manure in a chicken house, wherein the padding is prepared from mixed fermentation of basic windrow and a zymophyte agent, and 0.001-0.005% of folic acid, by mass, is added in the padding; the second step of adding the folic acid with mass percentage being 1-3% into chicken feed to obtain the folic acid-rich chicken feed, and feeding chickens with the folic acid-rich chicken feed, wherein the folic acid which is not absorbed is emitted along with the chicken manure; the third step of using the padding to degrade the chicken manure, and making the folic acid which is not absorbed enriched in the chicken house and used again so as to improve the egg folic acid content.

The invention discloses a lentil tea. The lentil tea consists of the following raw materials in parts by weight: 80-85 parts of lentil, 5-10 parts of buckwheat honey and 50-80 parts of drinking water.Meanwhile, the invention also discloses a preparation method of the lentil tea. The lentil tea provided by the invention has high folic acid content and less loss, the defect of much folic acid losscaused by traditional baking is overcome, the taste of the lentil tea is improved, and the palatability is improved. The finally prepared tea particles are porous and loose, and the lentil tea can fully release nutrient components and flavor substances in the soaking process.

The invention discloses barton padding capable of increasing the egg folic acid content and a preparation method thereof. The padding is mainly prepared from, by weight, 800-1,200 parts of basic stock, 1-10 parts of fermentation strains and 0.01-0.05 part of folic acid. The fermentation strains are mainly prepared from, by weight, 0.8-1.2 parts of candida utilis, 0.6-1.0 part of lactobacillus, 0.15-0.25 part of composite bacillus and 1-2 parts of photosynthetic bacteria. The basic stock and the fermentation strains are mixed and ferment, the folic acid is added, the barton padding containing active fermentation matter and rich in folic acid is obtained and laid in a barton, chicken manure can be degraded, a folic-acid-rich breeding environment is provided, and the folic acid content of eggs can be obviously increased.

The invention provides a lactic acid bacteria composition, which is composed of Streptococcus thermophilus inm25-ST, Lactobacillus delbrueckii subsp. bulgaricus inm25-LB and promoting factors. The composition is used to improve the folic acid metabolism capacity of Lactobacillus sake LZ217 during fermentation ; The advantages are: the production performance is good and stable, and it can be put into mass production in factories. At the same time, the obtained fermented product has good taste, texture and flavor, less whey precipitation during the shelf life, weak post-acidification, and high content of active folic acid.

The invention relates to nutrient porridge with millet as a raw material and aims at providing gingseng-poria cocos millet-rice porridge with relatively high folic acid content. A production method comprises the following steps: repeatedly freezing and unfreezing 15 parts of millet; taking appropriate amount of clear water, boiling, adding 15 parts by mass of rice, and cooking for 20-30 minutes; then adding the unfrozen millet and baking soda water, and boiling; and then adding 3 parts by mass of gingseng powder, 15 parts by mass of poria cocos powder and 30 parts by mass of Chinese yam powder, and stewing with small fire until the rice is rotten into porridge. According to traditional Chinese medicine theories, the gingseng-poria cocos millet-rice porridge has health care effect as the gingseng is a qi tonifying medicine and can be used for tonifying spleen and benefiting lung, the poria cocos can be used for clearing damp and promoting diuresis as well as tonifying spleen and calming the heart and the Chinese yam can be used for tonifying spleen and nourishing stomach as well as promoting production of body fluid and benefiting lung. Therefore, the porridge can be used for promoting diuresis and tonifying spleen and is applicable to patients suffering from spleen dampness.

The invention discloses application of OsAPBP2 protein to promotion of synthesis of plant folic acid. The OsAPBP2 protein provided by the invention is protein shown as a) or b) or c) or d): a) protein with an amino acid sequence shown as a sequence 2; b) fused protein obtained by connecting an end N and / or an end C of the protein shown as the sequence 2 with a label; c) protein which is obtained by carrying out substitution and / or deletion and / or addition of one or more amino acid residues on the amino acid sequence shown as the sequence 2, and has the same function; d) protein which has 75 percent or more than 75 percent of homology with the amino acid sequence shown as the sequence 2 and has the same function as the sequence 2. An experiment proves that the OsAPBP2 protein can be used for identifying and combining with rice folic acid to synthesize a key gene OsADCS promoter to regulate and control the expression of a rice folic acid synthesis key gene and further regulate and control the content of the folic acid in rice, and plays an important role in the synthesis of the plant folic acid.

The invention discloses a preparation method of a composite polysaccharide gel based on folic acid targeting, and belongs to the technical field of biology macromolecules. The preparation method comprises the following steps of enabling a coupling agent to modify acid polysaccharide, coupling with the folic acid, and performing in-situ releasing, so as to prepare a three-component composite polysaccharide gel with an interpenetration crosslinking network structure and high mechanical strength. The composite polysaccharide gel has the in-vitro drug-release targeting property based on the identification of folic acid receptor at the surface of tumor cell. The preparation method has the advantages that the coupling effect is good, and the conversion rate is high; the composite polysaccharidegel can load various anti-tumor drugs, and the application range of the natural macromolecules is widened.

The invention belongs to the technical field of selenium-enriched eggs and particularly relates to a laying hen feeding method for increasing the selenium content of eggs. The method comprises the following specific steps that from the age of 15 days of laying hens, a feeding material is fed twice a day, and a fermented feeding liquid is fed once. The feeding material is made of the following rawmaterials including fresh edible amaranth, fresh wheat leaves, fresh dandelion leaves, corn, tomato seed meal, activated medical stone powder, mesona blumes polysaccharide and curcumin. The fermentedfeed liquid is prepared according to the following steps that fresh sonchus oleraceus is washed, pulverized, subjected to constant temperature steaming and then taken out; carrots, purple grapes and water and added, beaten, fermented and then taken out, the mixture is magnetized and filtered, and the filtrate is taken to obtain the fermented feed liquid. Environmental-protection healthy productionof the laying hens and eggs is effectively promoted, the selenium content and folic acid content of the eggs are significantly increased, the nutritional and health-care value is increased, human health is promoted, the nutritional intake level of people's lives is increased, the egg weight can be effectively increased, and economic benefits are increased.

The invention belongs to the technical field of rice planting and in particular discloses a high-nutrition rice fertilization method which specifically comprises the following steps: in an early tillering period, performing root application of a urea nutrient liquid for one time, and performing leaf spraying of a leaf fertilizer for one time; in a medium spike differentiation period, performing root application of a copper sulfate nutrient liquid for one time, and performing leaf spraying of the leaf fertilizer for one time; in a medium earing period, performing root application of the urea nutrient liquid for one time, and performing leaf spraying of the leaf fertilizer for one time, wherein the leaf fertilizer is prepared from the following raw materials: copper chloride, borax, potassium fulvate, ferrous sulfate, grifolan, sophocarpidine, paeonol and water. By adopting the high-nutrition rice fertilization method, tillering of rice is effectively promoted, the number of roots of rice is increased, flower formation and flower retention are effectively promoted, differentiation of branches and glumous flowers is promoted, the number of the glumous flowers is increased, accumulation of stem and sheath reserve substances is improved, functions of nourishing roots, strengthening leaves, strengthening stems and preventing lodging are achieved, the number of grains is increased, the number of empty seeds is reduced, the yield of rice is increased, the contents of proteins, zinc and folic acid of the rice are remarkably increased, and the nutrition values of the rice are increased.

The invention discloses a method for producing folic acid function-enhanced eggs through nutrient interaction. A premix for feeding laying hens is prepared from folic acid, vitamin E, vitamin B1, vitamin B2, vitamin B12, xanthophyll, amino acid zinc, eucommia ulmoides extract and cinnamon extract. The preparation method of the eggs comprises the following steps that a premix is added into complete formula feed for laying hens to obtain chicken feed, and the additive amount of the premix in the complete formula feed for the laying hens is 1.0%; after the selected laying hens in the expected laying period are fed with chicken feed for 3 weeks, eggs produced by the laying hens in the laying period are rich in folic acid and various nutrients and are eggs with the folic acid strengthening function, and the egg laying peak period is ended; according to the eggs produced through the method, the yolk is orange, the Rogowski colorimetric value is 11-12 levels, it is detected that the folic acid content is increased by 2.1-2.4 times compared with that of common eggs, the content of other nutrients is increased by 1.49-3.89 times, and the functions of folic acid and multiple nutrients are enhanced through the complementary synergistic effect of the nutrients.

The invention relates to the plant genetic engineering field, in particular to a soybean folic acid synthesis key enzyme ADCS and a gene and application thereof. The amino acid sequence of the soybean folic acid synthesis key enzyme ADCS is shown in SEQ ID No.1 through the gene expression of the soybean folic acid synthesis key enzyme ADCS, the content of the folic acid synthetic precursor p-aminobenzoic acid (pABA) in the transgenic plant can be significantly increased. Therefore, the soybean folic acid synthesis key enzyme ADCS lays a foundation for the increase of the folic acid content of the plant, ensures that human can directly obtain natural folic acid through diet to meet daily need and has a significance to relieve the folic acid lack of human.

The invention relates to the field of plant genetic engineering, in particular to a method for increasing plant folic acid content by utilizing the synergistic effect of transgenic soybean GTPCHI and ADCS genes. According to the present invention, the gene sequence of the soybean folic acid synthesis key enzyme GTPCHI is shown in SEQ ID No.1, and the sequence of the ADCS gene is shown in SEQ ID No.2. The synergistic effect of transgenic soybean GTPCHI and ADCS genes can significantly increase the content of folic acid in transgenic plants, therefore, the present invention can be used as a method for producing high-content folic acid transgenic plants, and humans can directly obtain natural folic acid by eating such plants. It solves the lack of folic acid intake and overcomes the adverse side effects caused by taking folic acid tablets for a long time.

The invention relates to nutritious porridge with millet as a main material and aims at providing lotus root, lily and loquat porridge with the millet as the main material, and the high folic acid content is guaranteed. A making method of the lotus root, lily and loquat porridge comprises the steps that 100 parts of millet is taken; according to a mass ratio, 50 parts of lotus roots are taken and sliced to be added into water and stewed for 20-50 min; then, the unfrozen millet and baking soda water are added into the mixture; according to the mass ratio, 30 parts of fresh lily and 30 parts of peeled fresh loquats with seeds being removed are added into the mixture to be boiled, then, soft fire is used for boiling the mixture until the mixture becomes sticky. By means of the traditional Chinese medicinal principle and the traditional Chinese medicine principle, the lotus root, lily and loquat porridge has the effect of health maintaining; the lily can strengthen the middle warmer, moisten the lung and calm coughs; the loquats can moisten dryness, clear away the lung-heat, relieve coughs and calm the adverse-rising energy; the lotus roots can moisten dryness.