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Lactic acid bacterium composition and application thereof

A composition, lactic acid bacteria technology, applied in application, Lactobacillus, Streptococcus/Lactococcus and other directions, can solve the problems of low folic acid yield, poor water retention capacity, low viscosity, etc., and achieve strong folic acid metabolism, soft and silky texture. , the effect of increasing folic acid content

Active Publication Date: 2019-10-22
ZHEJIANG INM FOOD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] For industrial production strains, the breeding of strains with excellent production performance such as aroma production (acetaldehyde, diacetyl and other flavor substances), stickiness production (exopolysaccharide polymers, etc.), anti-post-acidification and the combination of excellent performance strains are the key factors. One of the keys to the industrialization of yogurt starter. In recent years, the common problems of fermented milk products are that industrially produced yogurt products have thin texture, low viscosity, poor water retention capacity, and a large amount of whey is easily produced during transportation and storage. The phenomenon of precipitation seriously affects product quality, so most dairy products on the market use thickener ingredients such as agar, pectin or hydroxypropyl distarch phosphate to improve the quality of yogurt
[0004] At present, there is a patent number: CN201510311918.2 A method for producing fermented soy milk with a high-yielding lactic acid bacteria strain. Lactobacillus sake LZ217 has the ability to metabolize folic acid. Because the fermented soy milk fermented by probiotics has a special flavor of beans, most people Not very acceptable, but when fermented in cow's milk, folic acid production is very low, limiting its application
Lactobacillus sake LZ217 has the problem of bad taste of fermented soybean milk and low folic acid production in cow milk
The existing production strains have poor production performance such as aroma production, stickiness production, post-acidification resistance, etc.

Method used

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  • Lactic acid bacterium composition and application thereof
  • Lactic acid bacterium composition and application thereof
  • Lactic acid bacterium composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1 A lactic acid bacteria composition, consisting of Streptococcus thermophilus inm25-ST, Lactobacillus delbrueckii subsp. bulgaricus inm25-LB and a promoting factor, the composition is used to improve the folic acid metabolism capacity of Lactobacillus sake LZ217 during fermentation .

[0022] A lactic acid bacteria composition, Lactobacillus delbrueckii subsp. bulgaricus, the strain name is inm25-LB, the preservation number of the strain is CGMCC No.15445, the preservation date is: March 12, 2018, and the preservation unit is: China Microbial Bacteria The Ordinary Microbiology Center of the Preservation Management Committee, the deposit address is: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

[0023] The sequence of Lactobacillus delbrueckii subsp. bulgaricus inm25-LB provided by the present invention is shown.

[0024] gagtttgatc ctggctcatg acgatcgctg gcggcgtgcc taatacatgc aagtcgagcgagctgaattc aaagattcct tcggggtgat ttgttggatg ctagcggcgg a...

Embodiment 2

[0036] Embodiment 2 The strain screening method of Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus

[0037] Screening of excellent mucilage-producing and aroma-producing Streptococcus thermophilus

[0038] Taking the texture and viscosity of single-strain fermented milk, whey precipitation after simulated transportation, and flavor production as indicators, 4 strains were selected from more than 200 strains of Streptococcus thermophilus as the alternative Streptococcus thermophilus strains.

[0039] Screening of Lactobacillus delbrueckii subsp. bulgaricus with excellent post-acidification performance

[0040] Taking the low temperature (4°C 7d) acidity change and the fermentation end point (80oT) time of single-strain fermented milk as indicators, 4 strains were selected from more than 100 strains of Lactobacillus delbrueckii subsp. bulgaricus as the alternative Lactobacillus delbrueckii bulgaricus. subspecies strains.

[0041] Screening of composi...

Embodiment 3

[0043] The preparation of embodiment 3 lactic acid bacteria composition

[0044] 1. Culture and count of fermented lactic acid bacteria

[0045] The Streptococcus thermophilus inm25-ST and Lactobacillus delbrueckii subsp. bulgaricus inm25-LB stored in glycerol tubes were activated and cultured for 2 to 3 times, respectively, and the Streptococcus thermophilus strain inm25-ST was added to M17 liquid medium at 42°C. After culturing for 16h, Lactobacillus delbrueckii subsp. bulgaricus inm25-LB was added to MRS liquid medium, cultured at 42°C for 20h, under aseptic conditions, centrifuged at 3000rpm for 6min to precipitate the cells, remove the supernatant, and collect the cells. The bacterial counts were calculated separately.

[0046] 2. Preparation of strain composition

[0047] According to the bacterial count, the Streptococcus thermophilus inm25-ST and the Lactobacillus delbrueckii subsp. bulgaricus inm25-LB were mixed according to the bacterial count content of 1000:1 to ...

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Abstract

The invention provides a lactic acid bacterium composition which is prepared from streptococcus thermophilus inm25-ST, lactobacillus delbrueckii subsp. bulgaricus inm25-LB and a promoting factor. Thecomposition is used for improving folic acid metabolism capability of the lactobacillus sobrinus LZ217 during fermentation and has the advantages that the production performance is good and stable, and the lactic acid bacterium composition can be put into a factory for mass production. In addition, the obtained fermented product has good taste, texture and flavor, less whey separation in a qualityguarantee period, weak post-acidification and high active folic acid content.

Description

technical field [0001] The present invention relates to a lactic acid bacteria composition and its application. Background technique [0002] Yogurt is a traditional fermented dairy product with rich nutrition and good probiotic health value, plus a thick and silky taste, and easy to digest and absorb. It is deeply loved by people. In recent years, with the improvement of our people's consumption level And the popularity of healthy ideas, yogurt products are growing at a rate of 25% per year. Yogurt starter is a key element in yogurt production, and its quality directly affects the sensory quality and flavor of the product. [0003] For industrial production strains, the selection of strains with excellent production performance such as aroma-producing (flavor substances such as acetaldehyde and diacetyl), viscosity-producing (exopolysaccharide polymers, etc.), and resistance to post-acidification, and the combination of strains with excellent performance are: One of the k...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23C9/123C12R1/46C12R1/225
CPCC12N1/20A23C9/1238A23C9/1234C12R2001/225C12N1/205C12R2001/46A23V2400/123A23V2400/249
Inventor 王伟军杨欢东陈波于靓朱立科任大喜刘建新
Owner ZHEJIANG INM FOOD
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