A kind of lactic acid bacteria composition and application thereof

A composition and technology of lactic acid bacteria, applied in the direction of application, lactobacillus, streptococcus/lactococcus, etc., can solve the problems of low folic acid production, poor water retention capacity, low viscosity, etc., and achieve strong folic acid metabolism, excellent production performance, and folic acid The effect of content increase

Active Publication Date: 2021-11-09
ZHEJIANG INM FOOD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] For industrial production strains, the breeding of strains with excellent production performance such as aroma production (acetaldehyde, diacetyl and other flavor substances), stickiness production (exopolysaccharide polymers, etc.), anti-post-acidification and the combination of excellent performance strains are the key factors. One of the keys to the industrialization of yogurt starter. In recent years, the common problems of fermented milk products are that industrially produced yogurt products have thin texture, low viscosity, poor water retention capacity, and a large amount of whey is easily produced during transportation and storage. The phenomenon of precipitation seriously affects product quality, so most dairy products on the market use thickener ingredients such as agar, pectin or hydroxypropyl distarch phosphate to improve the quality of yogurt
[0004] At present, there is a patent number: CN201510311918.2 A method for producing fermented soy milk with a high-yielding lactic acid bacteria strain. Lactobacillus sake LZ217 has the ability to metabolize folic acid. Because the fermented soy milk fermented by probiotics has a special flavor of beans, most people Not very acceptable, but when fermented in cow's milk, folic acid production is very low, limiting its application
Lactobacillus sake LZ217 has the problem of bad taste of fermented soybean milk and low folic acid production in cow milk
The existing production strains have poor production performance such as aroma production, stickiness production, post-acidification resistance, etc.

Method used

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  • A kind of lactic acid bacteria composition and application thereof
  • A kind of lactic acid bacteria composition and application thereof
  • A kind of lactic acid bacteria composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 A lactic acid bacteria composition, consisting of Streptococcus thermophilus inm25-ST, Lactobacillus delbrueckii subsp. bulgaricus inm25-LB and promoting factors, the composition is used to improve the folic acid metabolism capacity of Lactobacillus sake LZ217 during fermentation .

[0022] A lactic acid bacteria composition, Lactobacillus delbrueckii subspecies bulgaricus, the strain name is inm25-LB, the preservation number of the strain is CGMCC No.15445, the preservation date is: March 12, 2018, and the preservation unit is: China Microbial Strains General Microbiology Center of the Preservation Management Committee, the preservation address is: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing.

[0023] The sequence of Lactobacillus delbrueckii subsp. bulgaricus inm25-LB provided by the present invention is shown.

[0024] gagtttgatc ctggctcatg acgatcgctg gcggcgtgcc taatacatgc aagtcgagcgagctgaattc aaagattcct tcggggtgat ttgttggatg ctagcggcg...

Embodiment 2

[0036] The bacterial strain screening method of embodiment 2 Streptococcus thermophilus and Lactobacillus delbrueckii subspecies

[0037] Screening of Excellent Streptococcus Thermophilus and Aroma-producing Streptococcus

[0038] Using the single-strain fermented milk texture, viscosity, whey precipitation after simulated transportation, and flavor production as indicators, 4 strains were selected from more than 200 strains of Streptococcus thermophilus as candidate Streptococcus thermophilus strains.

[0039] Screening of Lactobacillus delbrueckii subsp. bulgaricus with excellent post-acidification performance

[0040] Taking the low-temperature (4°C 7d) acidity change and fermentation end point (80oT) time of single-strain fermented milk as indicators, 4 strains were selected from more than 100 strains of Lactobacillus delbrueckii subsp. subspecies strain.

[0041] Screening of compositions that promote the growth of Lactobacillus sake lz217 and the metabolism of folic ac...

Embodiment 3

[0043] The preparation of embodiment 3 lactic acid bacteria composition

[0044] 1. Cultivation and counting of fermenting lactic acid bacteria strains

[0045] Streptococcus thermophilus inm25-ST and Lactobacillus delbrueckii subsp. bulgaricus inm25-LB preserved in glycerol tubes were respectively activated for 2 to 3 times, and the Streptococcus thermophilus strain inm25-ST was inserted into M17 liquid medium at 42°C After culturing for 16 hours, Lactobacillus delbrueckii subsp. bulgaricus inm25-LB was inserted into MRS liquid medium, and cultured at 42°C for 20 hours. Under sterile conditions, the cells were centrifuged at 3000 rpm for 6 minutes, the supernatant was removed, and the cells were collected. Calculate the bacterial count separately.

[0046] 2. Preparation of strain composition

[0047] According to the bacterial count, Streptococcus thermophilus inm25-ST and Lactobacillus delbrueckii subsp. bulgaricus inm25-LB were mixed according to the bacterial count of 1...

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Abstract

The invention provides a lactic acid bacteria composition, which is composed of Streptococcus thermophilus inm25-ST, Lactobacillus delbrueckii subsp. bulgaricus inm25-LB and promoting factors. The composition is used to improve the folic acid metabolism capacity of Lactobacillus sake LZ217 during fermentation ; The advantages are: the production performance is good and stable, and it can be put into mass production in factories. At the same time, the obtained fermented product has good taste, texture and flavor, less whey precipitation during the shelf life, weak post-acidification, and high content of active folic acid.

Description

technical field [0001] The invention relates to a lactic acid bacteria composition and application thereof. Background technique [0002] Yogurt is a traditional fermented dairy product, which is rich in nutrition and good health care value. It has a thick and silky taste, and is easy to digest and absorb. It is deeply loved by people. In recent years, with the improvement of our people's consumption level With the popularity of health concepts, yogurt products are growing at a rate of 25% every year. Yogurt starter is a key element in yogurt production, and its quality directly affects the sensory quality and flavor of the product. [0003] For industrial production strains, the breeding of strains with excellent production performance such as aroma production (acetaldehyde, diacetyl and other flavor substances), stickiness production (exopolysaccharide polymers, etc.), anti-post-acidification and the combination of excellent performance strains are the key factors. One o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23C9/123C12R1/46C12R1/225
CPCC12N1/20A23C9/1238A23C9/1234C12R2001/225C12N1/205C12R2001/46A23V2400/123A23V2400/249
Inventor 王伟军杨欢东陈波于靓朱立科任大喜刘建新
Owner ZHEJIANG INM FOOD
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