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Preparation method of HPEI (hyperbranched polyethyleneimine)-encapsulated iron oxide magnetic nano particles

A magnetic nanoparticle and nanoparticle technology, applied in the fields of magnetic properties of inorganic materials, preparations for in vivo tests, and pharmaceutical formulations, etc.

Inactive Publication Date: 2013-02-06
DONGHUA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Retrieval of Fe related to HPEI protection at home and abroad 3 o 4 Literature and patent results on nanoparticle synthesis show that HPEI-protected Fe 3 o 4 Nanoparticles and the research on their surface modification and modification have not been reported yet.

Method used

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  • Preparation method of HPEI (hyperbranched polyethyleneimine)-encapsulated iron oxide magnetic nano particles
  • Preparation method of HPEI (hyperbranched polyethyleneimine)-encapsulated iron oxide magnetic nano particles
  • Preparation method of HPEI (hyperbranched polyethyleneimine)-encapsulated iron oxide magnetic nano particles

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Experimental program
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Effect test

Embodiment 1

[0065] 1.2519g FeCl 2 4H 2 O was added to the beaker, 7.75mL of ultrapure water (resistivity greater than 18.2MΩ.cm) was added, and 6.25mL of NH 3 ·H 2 O, the above-mentioned mixed solution was continuously stirred in the air for 10 minutes to ensure that the ferrous iron was fully oxidized, and then the reactant was transferred to the reaction kettle. Dissolve 0.53879g HPEI in 5mL aqueous solution, ultrasonically dissolve it, transfer it to the reaction kettle with a pipette gun, mix well with the solution in the reaction kettle, and react at 134~140℃ for about 3 hours; after the reaction, the natural After cooling to room temperature, the resulting black precipitated Fe 3 o 4 / HPEI is dispersed in ultrapure water, magnetically separated, redispersed, and magnetically separated, and washed with pure water five times to remove excess reagents, and redispersed in the water phase to obtain polyethyleneimine-protected tetraoxide Tri-iron nanoparticles (No.1 Fe 3 o 4 / HPEI);...

Embodiment 2

[0068] (1) Add 0.252g FeCl 2 4H 2 Add O into the beaker, then add 12.75mL of ultrapure water (resistivity greater than 18.2MΩ.cm), and add 1.25mL of NH 3 ·H 2 O, the above-mentioned mixed solution was continuously stirred in the air for 10 minutes to ensure that the ferrous iron was fully oxidized, and then the reactant was transferred to the reaction kettle. Dissolve 0.05153g HPEI in 5mL aqueous solution, ultrasonically dissolve it, transfer it to the reaction kettle with a pipette gun, mix well with the solution in the reaction kettle, and react at 134~140℃ for about 3 hours; refer to Table 1; After finishing, naturally cool to room temperature, the black precipitated Fe that obtains 3 o 4 / HPEI is dispersed in ultrapure water, magnetically separated, redispersed, and magnetically separated, and washed with pure water five times to remove excess reagents and redispersed in the water phase. The polyethyleneimine-wrapped ferric oxide nanoparticles No. 2 Fe 3 o 4 / HPEI; ...

Embodiment 3

[0071] (1) Add 0.25294g FeCl 2 4H 2 Add O into the beaker, add 12.75mL of ultrapure water (resistivity greater than 18.2MΩ.cm), and add 1.25mL of NH 3 ·H 2 O, the above-mentioned mixed solution was continuously stirred in the air for 10 minutes to ensure that the ferrous iron was fully oxidized, and then the reactant was transferred to the reaction kettle. Dissolve 0.1037g of HPEI in 5mL of aqueous solution, ultrasonically dissolve it, transfer it into the reactor with a pipette gun, mix well with the solution in the reactor, and react at 134~140°C for about 3 hours; refer to Table 1;

[0072] (2) After the reaction, cool down to room temperature naturally, and remove the obtained black precipitate Fe 3 o 4 / HPEI is dispersed in ultrapure water, magnetically separated, redispersed, and magnetically separated, and washed with pure water five times to remove excess reagents and redispersed in the water phase. The polyethyleneimine-wrapped ferric iron oxide nanoparticles No....

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Abstract

The invention relates to a preparation method of HPEI (hyperbranched polyethyleneimine)-encapsulated ferroferric oxide magnetic nano particles, comprising the steps of: adding Fe source to ultrapure water, then adding NH3.H2O, stirring in air, adding HPEI, and reacting at 134-140 DEG C for 3h, wherein the mass ratio of Fe source to HPEI is 1-5 :1; cooling, and carrying out washing and magnetic separation on precipitate to obtain HPEI-encapsulated ferroferric oxide nano particles Fe3O4 / HPEI; and then carrying out different surface modification on the Fe3O4 / HPEI nano particles, such as polyethylene glycolation (PEGlation), acetylation and carboxylation, so as to increase biocompatibility of the nano particles to be used for MRI (magnetic resonance imaging) diagnosis. The process is simple, the reaction conditions are mild and operation and separation are easy; and the prepared iron oxide magnetic nano particles have excellent biocompatibility and T2 relaxation effect, and have potential application value in the field of MRI imaging diagnosis.

Description

technical field [0001] The invention belongs to the field of preparation of iron oxide magnetic nanoparticles, in particular to a preparation and modification method of HPEI-wrapped iron oxide magnetic nanoparticles. Background technique [0002] In recent years, Fe3O4 magnetic nanoparticles (Fe 3 o 4 ) are widely used in various biomedical fields, such as cell separation and purification, magnetically controlled anticancer drug delivery, tumor magnetic hyperthermia, and magnetic resonance imaging (MRI). So far, Fe 3 o 4 It can be roughly divided into two synthetic methods, liquid-phase method and solid-phase method. The difference is that the liquid-phase method carries out the synthesis reaction in the liquid phase, while the solid-phase method can generate the target product without a solution process. The solid phase method is divided into ball milling method and high temperature thermal decomposition method. However, the purity of the prepared samples is not high, ...

Claims

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Application Information

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IPC IPC(8): C08G83/00H01F1/11H01F1/36A61K49/12A61K49/18
Inventor 史向阳蔡红东沈明武崔君张贵祥李康安郑林丰
Owner DONGHUA UNIV
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