Multi-mode targeted probe for early hepatic fibrosis diagnosis and preparation method thereof

A liver fibrosis, multi-mode technology, applied in the field of multi-mode targeting probes and its preparation, to achieve the effects of improving water solubility, reducing steric hindrance, and prolonging circulation time

Inactive Publication Date: 2011-03-30
ZHONGSHAN HOSPITAL FUDAN UNIV +1
View PDF3 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In liver fibrosis, there are no related research reports

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Multi-mode targeted probe for early hepatic fibrosis diagnosis and preparation method thereof
  • Multi-mode targeted probe for early hepatic fibrosis diagnosis and preparation method thereof
  • Multi-mode targeted probe for early hepatic fibrosis diagnosis and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Such as figure 1 Shown is the synthetic route map of the multimodal targeting probe of the present invention, the synthetic steps of the synthetic route map of the multimodal targeting probe (Den-RGD) are as follows:

[0046] 1. Synthesis of c(RGDyK):

[0047] The present invention synthesizes c(RGDyK) (MW=619.68Da) with Fmoc-protected solid-phase polypeptide synthesis method:

[0048] (1) Weigh 0.4g of resin-Gly (Fmoc-Gly-2-Cl-Trt, degree of substitution 0.6mmol / g, about 0.25mmol), wash and swell with dichloromethane (DCM) for 20 minutes, wash with DMF (N , N-dimethylformamide) and washed several times.

[0049] (2) Deprotection with 20% piperidine (DMF: piperidine = 2ml: 0.5ml) for 2 consecutive times, 10-15 minutes each time. DMF washed several times.

[0050] (3) Weigh 1.25 mmol of the required amino acid, add 0.18 g of HOBt (1-hydroxybenzotriazole), and use 2.45 ml of HBTU (benzotriazole-N, N, N', N'-tetra Methylurea hexafluorophosphate) and 0.44ml of DIEA (N,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a multi-mode targeted probe for early hepatic fibrosis diagnosis and a preparation method thereof. The multi-mode targeted probe for the early hepatic fibrosis diagnosis is characterized by comprising a vector, and the vector is connected with a fluorescence group and a magnetic resonance imaging group and can be connected with a targeted compound group, which can be combined with a receptor on the surface of a hepatic stellate cell in hepatic fibrosis/cirrhosis, through a bridging group. The preparation method comprises the steps of: connecting the targeted compound group, which can be combined with the receptor on the surface of the hepatic stellate cell in the hepatic fibrosis/cirrhosis, with the bridging group, and sequentially connecting the targeted compoundgroup, the fluorescence group and the magnetic resonance imaging group on the vector. The targeted probe has higher sensitivity when being used for diagnosing and evaluating the fibrosis diagnosis.

Description

technical field [0001] The invention relates to a multi-mode targeting probe for early diagnosis of liver fibrosis and a preparation method thereof, which is used for evaluating liver fibrosis by multi-mode non-invasive imaging of integrin αvβ3 receptors such as magnetic resonance and optics. Background technique [0002] To date, liver biopsy remains the only "gold standard" for assessing liver fibrosis. However, liver biopsy is invasive and can cause fatal complications, so it is not universally accepted by patients, which causes a large proportion of patients with chronic liver disease to delay diagnosis and treatment; meanwhile, in asymptomatic patients, It is difficult to repeat liver biopsy, which prevents accurate follow-up of changes in patients with chronic liver disease; in addition, sample sampling errors and inconsistencies in pathological observations all affect the accuracy of liver biopsy. Therefore, there is a need for an accurate and non-invasive method to ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/12A61K49/06C07K7/06
Inventor 王吉耀李聪严蕙蕙
Owner ZHONGSHAN HOSPITAL FUDAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap