31results about How to "Reduce steric hindrance" patented technology

The present invention relates to polymeric reagents and conjugates thereof, methods for synthesizing the polymeric reagents and conjugates, pharmaceutical compositions comprising the conjugates and methods of using the polymer conjugates including therapeutic methods where conjugates are administered to patients.

The invention provides nucleotide analogs for use in sequencing nucleic acid molecules.

A device, system and method for molecular computing which not only includes a suitable, renewable power source, but actually is able to receive power through the performance of the computations themselves. The molecular computing machine of the present invention actually employs the free-energy difference between its input and output to accomplish a computation, preferably by using its input DNA molecule as a partial source of energy, or alternatively by using the input DNA molecule as the sole source of energy. This molecular finite automaton preferably transforms an input DNA molecule into an output DNA molecule by digesting the input as it computes.

The invention provides a method for detecting a hapten in a sample comprising the steps of: a) providing a sample potentially containing the hapten; b) providing a pre-determined amount of a first moiety, said first moiety being bound to a signaller and separated therefrom by a first linker, which first moiety is either: i) a binding partner that specifically binds to the hapten of interest, or ii) the hapten of interest or an analogue thereof; wherein said signaller is a macromolecule or a nanoparticle providing high mass signal; c) providing a flow of a) and b) separately or together to an immobilised second moiety, said second moiety being bound to the surface of a sensor and separated therefrom by a second linker, which second moiety is either: i) a binding partner that specifically binds to the hapten of interest, or ii) is the hapten of interest or an analogue thereof, providing that when the first moiety is a binding partner, the second moiety is a hapten or hapten analogue and when the first moiety is a hapten or hapten analogue, the second moiety is a binding partner; and d) detecting the amount of first moiety bound to second moiety.

The invention relates to a multi-mode targeted probe for early hepatic fibrosis diagnosis and a preparation method thereof. The multi-mode targeted probe for the early hepatic fibrosis diagnosis is characterized by comprising a vector, and the vector is connected with a fluorescence group and a magnetic resonance imaging group and can be connected with a targeted compound group, which can be combined with a receptor on the surface of a hepatic stellate cell in hepatic fibrosis/cirrhosis, through a bridging group. The preparation method comprises the steps of: connecting the targeted compound group, which can be combined with the receptor on the surface of the hepatic stellate cell in the hepatic fibrosis/cirrhosis, with the bridging group, and sequentially connecting the targeted compoundgroup, the fluorescence group and the magnetic resonance imaging group on the vector. The targeted probe has higher sensitivity when being used for diagnosing and evaluating the fibrosis diagnosis.

The invention provides a preparation method of a formaldehyde adsorption water-based environmental protection coating. The preparation method includes the following steps that 1, styrene-acrylate resin liquid, pure acrylic emulsion, polyether-modified polysiloxane and poly(N-isopropylacrylamide)modified magnesium hydrate are added to deionized water, the mixture is heated and stirred to be uniform and filtered, and filtrate is obtained for use; 2, acrylate-styrene-vinyl acetate terpolymer, nanometer activated carbon, nanometer titania, calcined kaolin and an anion additive are added to a ball mill to be ground till the particle size is 60-80 nm, then the mixture is mixed and stirred, and a mixture is obtained; 3, the mixture obtained in step 2 and remaining hydroxyethyl cellulose, cellulose ether, 2-imidazolidone, 1-(2-hydroxyethyl)-2-imidazolidone, lauric acid and glycerin are added to the filtrate obtained in step 1, the mixture is mixed under the action of a high-speed blender and stirred for 30-40 minutes, then vinyl acetate-ethylene copolymer emulsion is added, the mixture stands still for 3-4 hours, and the formaldehyde adsorption water-based environmental protection coating is obtained.

Methods and compositions for quantitative immunoassays are provided, in which ligand-conjugated probes are used to label samples and ligand-surfaced microspheres are used as quantitative reference standards. Certain embodiments provide a method of quantitative flow cytometry where ligands are oligonucleotides, and a sample comprising one or more cells is contacted with a hybridized antibody::fluorophore labeled targeting construct to label the cells, and the labeled cells are analyzed. In some embodiments, a population of quantitative labeled oligospheres labeled with the same fluorescent label as the cells is analyzed using the flow cytometer and used to create a quantitative standard curve of cytometer intensity versus molecules fluorescent label per oligosphere event. A standard curve trendline is established and used to determine the molecules of fluorescent label per cellular event for the antigen-positive cell populations. Based on molecules of fluorescent label per cell, the amount of Antibody Binding per Cell (ABC) is quantified.

The invention provides a Sundan red I immunoaffinity column which comprises a carrier, protein G and a Sundan red I antibody, wherein the protein G is coupled with the carrier through chemical bonds; the Sundan red I antibody is in cross-link with the protein G by using a cross-linking agent after being joined; the protein G is recombinant protein G which is based on GenBank CAA27638.1 and is subjected to gene recombinant expression. Under the condition that detection properties are not affected, the use amount of the Sundan red I antibody of a sepharose gel carrier pretreated with the protein G is about 3/4 of that of an antibody for preparing purification column packing by using a direct coupling method. The Sundan red I provided by the invention is very high in purity, can be directly used in high performance liquid chromatography detection without need of extra subsequent purification treatment, therefore, the time and the cost of operators can be saved.

The invention discloses an acrylic plate with excellent bending performance and a preparation method thereof, relates to the technical field of acrylic plates, and aims to solve the problems of brittleness, low impact strength and poorer bending performance of an existing acrylic plate. The acrylic plate comprises a polymerization monomer, an initiator, a stabilizer, a plasticizer, reinforcing fibers and a flame retardant, the polymerization monomer is formed by quaternary copolymerization of methyl methacrylate, acrylic acid, butyl acrylate and diallyl adipate, and the reinforcing fibers comprise unidirectional borosilicate glass fibers and short carbon fibers. The initiator is one or a combination of azobisisovaleronitrile, azobisisobutyronitrile and benzoyl peroxide, the stabilizer is cuprous chloride and methyltin, the plasticizer is one or a combination of dibutyl phthalate, phthalate, sebacate, chlorinated paraffin and camphor, the flame retardant is one or a combination of is bromo-phosphate, and the coupling agent is one or a combination of bromophosphate, dimethyl methylphosphonate and diethylhexyl tetrabromo phthalate.

The invention relates to an ultra-deep desulfurization catalyst and a preparation method thereof. The preparation method comprises the following steps: (1) dissolving molybdate in deionized water to obtain a molybdate water solution, regulating the pH value to 1.0-5.0, adding a multi-tailed quaternary ammonium salt aqueous solution, regulating the pH value to 1.0-5.0, performing centrifugation, washing and drying to obtain a Mo-based precursor GSMPs; (2) dispersing the GSMPs in deionized water, adding a SiO2-Al2O3 carrier which is subjected to tabletting and sieving for reaction, performing washing and drying to obtain GSMPs/SA, and performing roasting to obtain an oxidation-state catalyst Mo-GSHD; (3) dipping the GSHD with a nickel salt solution, and performing drying and roasting to obtain an oxidation-state catalyst NiMo-GSHD; and (4) performing prevulcanization on the NiMo-GSHD to obtain the ultra-deep desulfurization catalyst. The catalyst is suitable for the modification of inferior fuel oil, is especially suitable for high-sulfur fuel oil, and can realize the ultra-deep desulfurization of fuel oil.

The invention relates to a noninvasive female corpus luteum function monitoring technology, in particular to a full-quantitative or semi-quantitative rapid noninvasive urine test technology for determination of cyclic production amount of progesterone metabolites in women's urine so as to monitor and evaluate a female corpus luteum function, judge different types of ovulation cycle, determine whether ovulation is really performed or not and impregnation can be achieved or not after ovulation. According to the first implementation scheme, a protein hybridized couplet for determining pregnanediol glucuronide is related and contains pregnanediol glucuronide and a coupling protein. The protein hybridized couplet is characterized by further containing a junctional complex that connects the two parts. PdG of the hormone protein couplet is detected without an unsaturated labelled antibody, the sensitivity can be up to 0.1 ng/ml, and the linear range can be controlled to be 0.1-50 ng/ml.

The invention provides a water-based fingerprint-resistant coating with an interpenetrating network structure for a galvanized steel sheet. The water-based fingerprint-resistant coating comprises 25-50% of a film-forming substance, 1-5% of a silane coupling agent, 1-5% of silica sol, 0.1-0.5% of a wetting agent, 0.1-0.5% of a leveling agent, 0.5-5% of a polyethylene wax dispersion and the balance of water, the film forming substance is formed by mixing water-based polyurethane resin with a linear structure and water-based acrylic resin with a net structure, or the film forming substance is formed by mixing water-based acrylic resin with a linear structure and water-based polyurethane resin with a net structure, and in the film forming substance, the ratio of water-based polyurethane resin to water-based acrylic resin is about 1: 2-2: 1. By mixing the polyurethane resin with the linear structure and the acrylic resin with the net structure or mixing the acrylic resin with the linear structure and the polyurethane resin with the net structure, the steric hindrance effect between the resins is reduced, an interpenetrating network structure of the linear structure and the net structure is formed, the compactness of the film layer is greatly improved, and the film layer is more compact. The performance indexes of salt fog resistance, acid and alkali resistance, solvent resistance, water resistance and the like of the fingerprint-resistant film layer are improved.