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A method for improving the efficiency of poplar wood enzymatic hydrolysis

A poplar enzymatic and enzymatic hydrolysis technology is applied in the field of biomass pretreatment and enzymatic hydrolysis, which can solve the problems of inhibiting cellulase hydrolysis ability, low enzymatic hydrolysis efficiency, and large amount of cellulase used, so as to reduce space obstacles. The effect of function, simple process and stable hydrolysis ability

Active Publication Date: 2021-01-15
NORTHWEST A & F UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0012] The purpose of the present invention is to solve the disadvantages of large amount of cellulase used in the existing poplar hydrolysis process and low enzymatic hydrolysis efficiency, and provide a method for improving the enzymatic hydrolysis efficiency of acetic acid hydrogen peroxide pretreatment poplar wood
[0014] Based on the existing technology, it is impossible to know why there is still a large amount of cellulase used and low enzymatic hydrolysis efficiency after pretreatment of poplar wood with acetic acid hydrogen peroxide, which is promising in application prospects. The inventors conducted in-depth research on this and finally found that acetic acid hydrogen peroxide After pretreatment of poplar, there will be a certain amount of cellobiose accumulation in the process of enzymatic hydrolysis, thereby inhibiting the hydrolysis ability of cellulase, leading to the above problems

Method used

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  • A method for improving the efficiency of poplar wood enzymatic hydrolysis
  • A method for improving the efficiency of poplar wood enzymatic hydrolysis

Examples

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Effect test

Embodiment 1

[0039] A method for improving poplar enzymatic hydrolysis efficiency, comprising the following steps:

[0040] Step 1) Preprocessing

[0041] 1.1) Mix 60 mL of acetic acid with a purity of 99% and 60 mL of hydrogen peroxide with a purity of 30%, add a catalytic amount of 100 mmol / L sulfuric acid, and mix well to prepare an acetic acid hydrogen peroxide pretreatment solution;

[0042] 1.2) Weigh 10 grams of dry poplar wood residue after acetic acid pretreatment and place it in a polytetrafluoroethylene-lined steel cylinder, add acetic acid hydrogen peroxide solution with a volume concentration of 100% according to the ratio of solid to liquid ratio of 1:10, and place it at 60°C to Pretreatment at 80°C for 2 hours; the above-mentioned acetic acid pretreatment refers to adding acetic acid with a purity of 5% to poplar and treating it at 170°C for 30 minutes;

[0043] 1.3) After the pretreatment, cool down, use a centrifuge to separate the solid and liquid, collect the filter res...

Embodiment 2

[0055] The pretreatment method and reaction conditions are basically the same as in Example 1, the difference is that the volume concentration of the acetic acid hydrogen peroxide solution in the acetic acid hydrogen peroxide pretreatment is 80%; if only 20FPU cellulase is added during hydrolysis, the calculated glucose yield is 67.2% , The yield of xylose was 72.4%. After adding 500nkat of β-glucosidase per gram of substrate, the yield of glucose hydrolyzed by the enzyme is increased to 91.1%, and the yield of xylose is increased to 94.9%.

Embodiment 3

[0057] The pretreatment method and reaction conditions are basically the same as in Example 1, the difference is that in the enzymatic hydrolysis process, 1000nkat of β-glucosidase is added per gram of substrate, and the final glucose yield is 97.5%, and the xylose yield is 99.7% .

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Abstract

The invention provides a method for improving enzymatic hydrolysis efficiency of poplar wood. Poplar wood pretreated with an acetic acid hydrogen peroxide solution is used as a raw material, and the problems of large usage quantity of cellulases and low enzymatic hydrolysis efficiency existing in an existing hydrolyzing process are solved. According to the method, based on the cellulases, beta-glucosidases is additionally added to an enzymatic hydrolysis reaction system, so that the highest glucose yield of the poplar wood can reach 99.4%, and the glucan in the poplar wood is nearly hydrolyzedcompletely. The method is high in efficiency, low in cost and simple and convenient to operate, complete enzymatic hydrolysis of the poplar wood is nearly realized, and a foundation is established for preparation of biological energy sources and chemicals through bioconversion of the poplar wood.

Description

technical field [0001] The invention relates to biomass pretreatment and enzymatic hydrolysis technology, in particular to a method for improving the enzymatic hydrolysis efficiency of poplar after pretreatment with acetic acid hydrogen peroxide by adding β-glucosidase. Background technique [0002] Poplar is a fast-growing and high-yielding tree species that has been planted in a large area in China. It has fast growth, rich resources, and high cellulose content. Therefore, the biotransformation of poplar has great potential for producing bioenergy and chemicals. Pretreatment is an important step to improve the enzymatic hydrolysis efficiency of poplar, and the pretreatment of poplar is mainly divided into acid, alkali, ionic liquid, steam explosion and other methods. [0003] Huang et al. in 2018, J Wood Chem Technol.38(5):371-384 treated with 6.5% acetic acid at 172°C for 27 minutes, added 20FPU / g dry matter cellulase, and the enzymatic hydrolysis yield of poplar was 51%...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14C12P19/02
Inventor 张军华文沛瑶张甜贾丽丽
Owner NORTHWEST A & F UNIV
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