Multi-mode targeted probe for early hepatic fibrosis diagnosis and preparation method thereof

A liver fibrosis, multi-mode technology, applied in the field of multi-mode targeted probes and its preparation, to achieve the effects of prolonging circulation time, reducing steric hindrance, and improving water solubility

Inactive Publication Date: 2013-01-23
ZHONGSHAN HOSPITAL FUDAN UNIV +1
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In liver fibrosis, there are no related research reports

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Multi-mode targeted probe for early hepatic fibrosis diagnosis and preparation method thereof
  • Multi-mode targeted probe for early hepatic fibrosis diagnosis and preparation method thereof
  • Multi-mode targeted probe for early hepatic fibrosis diagnosis and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Such as figure 1 Shown is a synthetic route map of the multi-modal targeting probe of the present invention, and the synthetic steps of the synthetic route map of the multi-modal targeting probe (Den-RGD) are as follows:

[0046] 1. Synthesis of c(RGDyK):

[0047] The present invention uses Fmoc-protected solid-phase peptide synthesis method to synthesize c(RGDyK) (MW=619.68Da):

[0048] (1) Weigh 0.4g resin-Gly (Fmoc-Gly-2-Cl-Trt, degree of substitution 0.6mmol / g, about 0.25mmol), rinse with dichloromethane (DCM) and swell for 20 minutes, use DMF (N , N-dimethylformamide) wash several times.

[0049] (2) Deprotection with 20% piperidine (DMF: piperidine=2ml:0.5ml) consecutively for 2 times, each time for 10-15 minutes. DMF wash several times.

[0050] (3) Weigh 1.25mmol of the required amino acid, and add 0.18g of HOBt (1-hydroxybenzotriazole), use 2.45ml of HBTU (benzotriazole-N, N, N', N'-tetrazolium Methylurea hexafluorophosphate) and 0.44ml of DIEA (N,N-diisopropylethylam...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a multi-mode targeted probe for early hepatic fibrosis diagnosis and a preparation method thereof. The multi-mode targeted probe for the early hepatic fibrosis diagnosis is characterized by comprising a vector, and the vector is connected with a fluorescence group and a magnetic resonance imaging group and can be connected with a targeted compound group, which can be combined with a receptor on the surface of a hepatic stellate cell in hepatic fibrosis / cirrhosis, through a bridging group. The preparation method comprises the steps of: connecting the targeted compound group, which can be combined with the receptor on the surface of the hepatic stellate cell in the hepatic fibrosis / cirrhosis, with the bridging group, and sequentially connecting the targeted compoundgroup, the fluorescence group and the magnetic resonance imaging group on the vector. The targeted probe has higher sensitivity when being used for diagnosing and evaluating the fibrosis diagnosis.

Description

Technical field [0001] The invention relates to a multi-mode targeting probe for early diagnosis of liver fibrosis and a preparation method thereof, which are used for evaluating liver fibrosis by using multi-mode non-invasive imaging of integrin αvβ3 receptors such as magnetic resonance, optics, etc. Background technique [0002] So far, liver biopsy is still the only "gold standard" for evaluating liver fibrosis. However, liver biopsy is invasive and can cause fatal complications, so it cannot be generally accepted by patients. This has caused a large number of patients with chronic liver disease to delay diagnosis and treatment; at the same time, in patients with no obvious symptoms, It is difficult to repeat liver biopsy, which makes it impossible to accurately follow up the changes of patients with chronic liver disease; in addition, sample sampling errors and inconsistencies in pathological observations affect the accuracy of liver biopsy. Therefore, an accurate non-invasi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/12A61K49/06C07K7/06
Inventor 王吉耀李聪严蕙蕙
Owner ZHONGSHAN HOSPITAL FUDAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap