140 results about "Cell sensitivity" patented technology

An implantable biosensor assembly and system includes an enzymatic sensor probe from which subcutaneous and interstitial glucose levels may be inferred. The assembly may be associated by direct percutaneous connection with electronics, such as for signal amplification, sensor polarization, and data download, manipulation, display, and storage. The biosensor comprises a miniature probe characterized by lateral flexibility and tensile strength and has large electrode surface area for increased sensitivity. Irritation of tissues surrounding the probe is minimized due to ease of flexibility and small cross section of the sensor. Foreign body reaction is diminished due to a microscopically rough porous probe surface.

A highly sensitive assay is disclosed which combines immunomagnetic enrichment with multiparameter flow cytometric and immunocytochemical analysis to detect, enumerate and characterize carcinoma cells in the blood. The assay can detect one epithelial cell or less in 1 ml of blood and has a greater sensitivity than conventional PCR or immunohistochemistry by 1-2 orders of magnitude. In addition, the assay facilitates the biological characterization and staging of carcinoma cells.

A powered dispenser for dispensing individual sheet segments from a continuous roll of sheet material provided with spaced tear lines comprises a powered feed mechanism, a releasable, powered drive mechanism, a powered transfer mechanism, a pair of web sensing sensors, a capacitive sensing system providing automatic sensitivity adjustment, and control circuitry. A dual power supply system provides a mechanical lock-out functionality, and the control system is protected from electrostatic build-up on the surface of the feed roller. The web sensor, and an antenna plate of the capacitive sensing system, are provided on respective printed circuit boards mounted in overlying relation. Utilizing signals received from the pair of web sensors and the capacitive sensing system, the control circuitry senses the presence of a user to activate the powered drive mechanism, and prevents further dispensing of the sheet material until a previously dispensed segment is separated from the roll. The web sensors detection of a leading edge of the sheet material initiates a predetermined interval of sheet material advancement providing a proper placement of successive tear lines. Various approaches may be utilized to accommodate inadvertent sheet “tabbing” scenarios. The web sensors, together with the control circuitry, are also used to detect the depletion, or absence, of a working roll of sheet material, whereupon the control circuitry controls the powered transfer mechanism to automatically transfer the web feed supply from a depleted working roll to a reserve roll. The powered transfer mechanism may include a motor driven transfer bar, or provide motor driven release of a spring biased transfer bar. Another arrangement allows for ready release of a roll core, and drop of the same into an open dispenser cover for removal.

The invention provides devices that improve tests for detecting specific cellular, viral, and molecular targets in clinical, industrial, or environmental samples. The invention permits efficient detection of individual microscopic targets at low magnification for highly sensitive testing. The invention does not require washing steps and thus allows sensitive and specific detection while simplifying manual operation and lowering costs and complexity in automated operation. In short, the invention provides devices that can deliver rapid, accurate, and quantitative, easy-to-use, and cost-effective tests.

A composition for the treatment of impaired glucose tolerance (IGT) including a compound which binds to a receptor for glucagon-like peptide-1, and a pharmaceutical carrier. The amount of the compound present is an effective amount to improve pancreatic β-cell sensitivity to blood glucose levels in a human with IGT. Also, a method for improving the pattern of insulin secretory responses in a human with IGT by administering to the human a composition comprising a compound which binds to a receptor for glucagon-like peptide-1 and a pharmaceutical carrier.

The present invention provides a method for detecting and differentiating disease states with high sensitivity and specificity. The method allows for a determination of whether a cellbased sample contains abnormal cells and, for certain diseases, is capable of determining the histologic type of disease present. The method detects changes in the level and pattern of expression of the molecular markers in the cell-based sample. Panel selection and validation procedures are also provided.

The present invention provides methods of predicting cell sensitivity or resistance to a therapeutic agent.

The invention provides an optical acoustic ultrasound excitation and induction integrated detection device. An acoustic insulating layer, a sound absorption pad, a miniaturized semiconductor laser tube, a Fourier lens, a cylinder lens, a protection film and an annular multi-ring array sensor are all arranged in casing of the detection device and at same axle wire to form an integrated coaxial confocal structure. The multi-ring array sensor can generate ultrasonic signal, receive ultrasonic echo signal and receive optical acoustic signal by time-sharing excitation to realize optical acoustic ultrasound single or combined detection of A-type dynamic scan. The detection device has advantages of integrating excitation and induction of optical acoustic ultrasound, greatly increasing excitation and induction efficiency optical acoustic ultrasound, effectively reducing laser energy demand, increasing detection depth, and having high measurement sensitivity, simple operation and low cost. The detection device can be widely applied in fields of blood oxygen detection, blood sugar detection, HIFU therapy and effect monitoring, industrial crack detection and so on.

The invention relates to the field of genetic engineering, and provides a method and a corresponding kit for detecting a lung cancer susceptibility gene. The method for detecting the lung cancer susceptibility gene comprises the following steps of: A, using a specificity primer of the lung cancer susceptibility gene to amplify a plurality of target areas in a sample to be detected, and establishing a sequencing library based on amplified products; B, subjecting the sequencing library to monomolecular amplification to obtain a plurality of monomolecular amplification products corresponding to the plurality of target areas; and C, subjecting the plurality of monomolecular amplification products to high-throughput gene sequencing at the same time so as to obtain sequence information of the plurality of target areas. The method and the corresponding kit are used for sequencing the plurality of areas of the lung cancer susceptibility gene at the same time by using a high-throughput sequencing technology; the sequence information including variation of known mutations and unknown mutations in the areas can be obtained accurately; the kit has high detection sensitivity, and can be used for further detecting mainly samples at the same time.

The invention discloses a leukemia fusion gene combined parallel detecting method and a diagnostic reagent kit. The combined parallel detecting method is characterized in that multiple fusion genes related to leukemia can be detected at one time. By designing a primer and a probe of the leukemia fusion gene mRNA, the mixture covalently combined by the probe and microspheres is hybridized with a reverse transcription PCR amplified product, the streptavidin of phycoerythrin is added, and then the fluorescence signals of different microspheres can be detected, thereby determining whether the sample to be detected contains leukemia fusion genes or not and the express conditions of the fusion genes. The diagnostic reagent kit has the advantages of high sensitivity, high flux property, quick and accurate detection, and the like, and can simultaneously carry out qualitative and quantitative detection on multiple leukemia fusion genes.

A sensor for monitoring the lambda of a component of a reactant feed stream flowing through a fuel cell stack. The sensor comprises one or more fuel cells that are sensitive to a change in lambda of a specific component of a reactant feed stream flowing through the fuel cell. The sensitivity of the fuel cell causes a voltage produced by the lambda sensing fuel cell to vary in response to variation in the lambda of the specific component. The variation of the voltage output can be modeled and / or compared to empirical data to correlate the voltage output to the lambda of the specific component. Based on the lambda of the specific component, the operation of the fuel cell stack can be optimized.

The invention discloses a recognition probe of cancer cells. The length of the recognition probe of the cancer cells is larger or equal to 70bp, and the recognition probe of the cancer cell comprises an nucleic acid aptamer which is combined with the cancer cells in a specific binding mode, and an extending sequence which is arranged at a 5' end or a 3' end of the nucleic acid aptamer, wherein the extending sequence is a single stranded nucleotide sequence which is not enabled to form a secondary structure by itself. The invention further provides a detection method of the cancer cells by using the recognition probe and a kit. The recognition probe of the cancer cells is simple in manufacture, free from immunogenicity, free from toxicity, and is good in chemical stability. The detection method of the cancer cells combines the recognition probe based on nucleic acid aptamer design with a nucleic acid amplification technology, is good in detection sensitivity, and is good in detection efficiency.

The present invention includes a highly sensitive method for detecting the presence of proteases in a sample which are present at very low levels.

The present invention provides a method for detecting and differentiating disease states with high sensitivity and specificity. The method allows for a determination of whether a cell-based sample contains abnormal cells and, for certain diseases, is capable of determining the histologic type of disease present. The method detects changes in the level and pattern of expression of the molecular markers in the cell-based sample. Panel selection and validation procedures are also provided.

Many applications in the study of metabolics and proteomics require measurements on peptides and small molecules with high resolving power and mass accuracy. These are often present in complex mixtures and sensitivity over a relatively broad mass range, speed of analysis, reliability, and ease of use are very important. The present invention is a time-of-flight mass spectrometer providing optimum performance for these and similar applications.

The invention discloses a non-small cell lung cancer targeted therapy gene detection method, and belongs to the field of gene detection. The method for detecting 466 mutations of 12 oncogenes is developed by multiplex PCR and high throughput sequencing technologies, wherein the oncogenes are AKT1, ALK, BRAF, EGFR, ERBB4, FGFR1, FGFR2, FGFR3 , KRAS, MET, PIK3CA, and PTEN, and the mutations may be substitutions, insertions and / or deletions of one or more bases. The detection method provided by the invention has the advantages of high detection sensitivity of up to 0.01%, and clear and objective detection results, can be directly used for reflecting specific mutation sites of the relevant gene, directly used for guiding clinical non-small cell lung cancer targeted dosage, and used for early diagnosis or auxiliary diagnosis and screening of cancers as well as post-cancer surveillance.

The present invention relates to methods of determining cancer cell sensitivity to treatment by correlating the pattern of sensitivity of the cell to a panel of BH3 domain peptides. The invention also provides a method applying an algorithm to said pattern to predict therapeutic efficacy and of monitoring the shift in cell sensitivity to a therapeutic during treatment.

The invention provides a full-current differential protection braking coefficient adaptive regulating method for a transmission line. The method comprises the following steps of: acquiring the current on the two sides of a line; and adaptively regulating a braking coefficient of full-current differential protection according to the intensity of a load current and whether the line is a weak feed line in four situations. By the method, the braking coefficient of the full-current differential protection is adjusted in real time according to the load of the line, so that the sensitivity of the full-current differential protection and the adaptability of protection criterion can be improved, and the action performance of a relay protection device can be improved.

A system and method for producing an oligonucleotide having a high affinity for extracellular or cell surface markers on a target cell. The resultant oligonucleotide probe can be used to detect a target biomolecule, in particular a cancer cell or infectious agent such as a bacterium, virus, or fungus, comprising an aptamer having a high affinity for the biomolecule, wherein at least one labeled dye is attached to the aptamer. The labeled dye causes the aptamer to emit a baseline, non-visible emission. When the aptamer (also referred to herein as a probe) of the invention interacts with a target biomolecule, the fluorescence emission changes from the baseline emission to an emission that is visually detectable.

The invention belongs to the technical field of analytical chemistry and a chemical sensor, and discloses an electrochemical sensor for diagnosis of an ovarian SKOV-3 cancer cell, relating to a preparation method of a sandwich-type immunosensor based on graphene and a DNA (deoxyribonucleic acid) marker and application of the same in medical diagnosis. Antigens (human ovarian cancer) are indirectly tested with a glassy carbon electrode modified by graphene / antibody / antigen / DNA-labeled antibody / complementary DNA through catalytic oxidation based on guanine bases and adenine bases. The high electronic conductivity of the graphene is adopted, and the cost of the antibody marker is reduced. The immunosensor prepared based on the technical scheme can also be applied in detection of other types of cells has a wide scope of application. The sensor has high sensitivity, can be prepared simply, can be applied in detection any cancer cell, and has good prospects of application in medical diagnosis.

The invention provides a two-photon fluorescent probe for detecting viscosity of positioning mitochondria and a synthesizing method and application thereof, and belongs to the field of small-moleculefluorescent probes. The structural formula of the two-photon viscosity probe is shown in the description; the probe can be prepared by reacting products of 3-bromine-9-ethyl carbazole and 4-formyl benzene boric acid and the products of 2, 3, 3-trimethyl-3H-benzpyrole and iodomethane. The probe has two-photon effect and is high in sensitivity relative to viscosity and is capable of detecting viscosity in a solution, cells and zebra fish body under a single-photon or a two-photon mode; and the probe has a wide application prospect in the field of biomolecule detection.

A fast detection method of microbe anti- interference includes using large sample method or MPN method to enrich bacteria for microbe culture media sample, processing prepared sample with cell ATP release agent Ec and adding luciferase - luciferin reagent and deinhibitor for ATP luminous detection, confirming that sample is bacteria carrier positive if sample luminous pulse counts CPMs is greater than control sample solution CPMck, checking MPN table to obtain bacteria content in sample. The method can raise detection sensitivity by 1000 times comparing to normal method.

The present invention relates to a microbial cell sensor for detecting arsenic bioavailability degree, which is suitable for detecting bioavailability degree of arsenic in water and soil. The microbial cell sensor comprises: Escherichia coli as a host cell carrying recombinant plasmid. The recombinant plasmid is pUC18 plasmid containing arsenic resistant system ars promoter, arsenic resistant system controlling gene arsR, luciferase gene luc and rrnb terminator tandem sequence. The response time of the sensor on the arsenic is 30 minutes, the lowest detection concentration of the arsenic is 0.01 micromole / L, the highest detection concentration is 100 micromole / L. The microbial cell sensor has features of high sensitivity, fast response speed, low cost, and simple operation, and can be widely used in detecting the arsenic polluting the environment and evaluating the risk.

The invention relates to the field of genetic engineering, and provides a method and a kit for assaying breast cancer susceptibility genes. The method for assaying breast cancer susceptibility genes includes the following steps: (A) breast cancer susceptibility gene specific primers are utilized to amplify a plurality of target regions in a sample to be assayed, and a sequencing library is constructed on the basis of amplification products; (B) the single-molecule amplification of the sequencing library is carried out, so that a plurality of single-molecule amplification products corresponding to the target regions are obtained; (C) the high-throughput gene sequencing of the single-molecule amplification products is carried out simultaneously, so that the sequence information of the target regions is obtained. The method and the kit for assaying breast cancer, which are provided by the invention, can assay a plurality of regions of a breast cancer susceptibility gene at the same time, consequently, the efficiency of assay is increased, the assay cost is reduced, and in addition, the sensitivity of assay and the comprehensiveness and accuracy of the assay result are increased as well.

The invention relates to a load which monitors a line online, carries out a real-time calculation to the changes of the resistance of the load, combines with the fixed value range of all resistance sections with distance protection and phase selectors to distinguish a plurality of practical situations such as different malfunction types, system oscillation identification of oscillation detection elements, sending end or receiving end, etc., and adjusts the elements in resistance range: the protection can not only avoid a load state and but also can ensure the reaction capability to transition resistance. The method carries out the load resistance judgement and comparison by adopting the data windows of two frequency currents and is not affected by the transitional data window; therefore, the invention can fast judge the changes of the load, thus improving the performance of distance protection real-time monitoring load and ensuring no misoperation of distance protection in over load; furthermore, the invention can reliably start the distance protection when a high-resistance earth fault occurs in the area, thus leading the protection to have higher sensitivity.

The invention discloses a MOS-pipe-based double-grid-regulation ultra-high-sensitivity biosensor, which is suitable for a series of early-stage tumour detection. The sensor is made from an SOI sheet,and a unique double-grid control structure is formed through ion implantation. The sensor is made via ultraviolet lithography combined with an NLD etching method and can detect a trace amount of a tumour marker immediately without labeling. Compared with a common sensor used for detecting current or conductance, the sensor can detect change of capacitance in a channel in an antigen-antibody combination process. A detection method related in the invention is stable and good in interference immunity, and can meet the requirements of detection range and sensitivity, has extremely outstanding performance particularly with respect to detection sensitivity, and can detect a sample with the lowest concentration in a range of 1 fg / ml-1 ng / ml.

The invention discloses a target cell staining kit for displaying the proliferative activity of a cell and a use method thereof. The kit comprises I type and II type which respectively comprise an enzyme substrate, test paper, enzyme stationary liquid, phosphate buffer solution, Mayer hematoxylin and the like. The staining method comprises the steps of preparing pieces, fixing, staining and the like. The method can display acid phosphatase in exfoliative cells on smears such as exfoliative cervical cells, esophagus brush sheet cells and the like and search abnormal proliferated cells according to the target. Compared with the traditional Pasteur smear method, the method has the advantages of higher sensitivity for detecting cancer and precancerous lesions and similar specificity, therefore, the invention can be used for cytology screening of cervical carcinoma, esophageal cancer, lung cancer and other malignant tumors.

The present invention relates provides methods of predicting cell sensitivity to a test agent. In some embodiments, the cells are cultured in a culture medium having serum.