Cell-based detection and differentiation of disease states

A cell and disease technology, applied in the field of early detection, can solve the problem of not finding the magic bullet probe and so on

Inactive Publication Date: 2005-11-09
MONOGEN INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Thorough exploration of "magic bullet" diagnostic tests has been underway for d

Method used

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  • Cell-based detection and differentiation of disease states
  • Cell-based detection and differentiation of disease states
  • Cell-based detection and differentiation of disease states

Examples

Experimental program
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preparation example Construction

[0118] Preparation of specimens for analysis involves spreading samples on glass slides using methods including, but not limited to, smears, centrifugation, or deposition of cell monolayers. These methods can be manual, semi-automatic or fully automatic. The cell suspension can be aspirated to allow the cells to settle on the filter, and the cell monolayer is transferred to a prepared glass slide and can be processed for further evaluation. This process can also be repeated to prepare additional slides as necessary. The invention includes the detection of one molecular marker per slide. It also involves detection of several molecular markers per slide. Preferably, 1-6 markers are detected per slide. In some embodiments, 2 markers are detected per slide. In other embodiments, 3 markers are detected per slide.

[0119] The present invention involves the detection of altered expression of molecular markers at the DNA, RNA or protein level using one of a number of methods ava...

Embodiment I-VI

[1147] VII. Summary of Examples I-VI

[1148] Examples I-VI above provide preferred probes / labels for inclusion in probe sets for the detection and / or diagnosis of lung cancer, colorectal cancer, bladder cancer, prostate cancer, breast cancer and cervical cancer. Figures 8a-c provide a summary of preferred probes / markers for each cancer type. Figures 8a-c also identify which markers are useful for general cancer detection purposes, and which markers are more valuable due to specificity for specific cancer types. For example, EGFR and Ki-67 can be used for general cancer detection. BL2-10D1, CD44v3, collagenase, COX-1, HLA-DR, HSP-90, IL-6, IL-10, Lewis X, AP-22, TGF-β1, TGF-1I, TGF1II and UBC can be used for bladder Detection and / or diagnosis of cancer, AE1 / AE3, BCA-225, BRCA-1, CA-15.3, cathepsin D, GCDFP-15, HOX-B3, p65, PR and TGK can be used for the detection and / or Diagnosis, Cyclin E, E6 and E7 can be used for the detection and / or diagnosis of cervical cancer, AKT, am...

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Abstract

The present invention provides a method for detecting and differentiating disease states with high sensitivity and specificity. The method allows for a determination of whether a cellbased sample contains abnormal cells and, for certain diseases, is capable of determining the histologic type of disease present. The method detects changes in the level and pattern of expression of the molecular markers in the cell-based sample. Panel selection and validation procedures are also provided.

Description

[0001] Cross References to Related Applications [0002] This application is a continuation-in-part of U.S. Application Serial No. 10 / 095,298, filed March 12, 2002, which claims the benefit of U.S. Provisional Application Serial No. 60 / 274,638, filed March 12, 2001, the entire contents of which are incorporated herein as refer to. Background of the invention [0003] The present invention relates to the early detection of general conditions in patients. The invention also relates to the differentiation of a particular pathology in its early and late stages. [0004] Early detection of specific pathological conditions can greatly increase a patient's chance of survival through early diagnosis and early treatment, while the disease remains localized and its pathological effects are limited anatomically, physiologically, and clinically. The two main metrics that can be evaluated for any test or disease detection method are sensitivity (sensitivity=true pos...

Claims

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Application Information

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IPC IPC(8): C07H21/04C12Q1/04C12Q1/68G01NG01N33/48G01N33/53G01N33/569G01N33/574
CPCC12Q1/6883C12Q1/6809G01N33/569G01N33/56966C12Q2600/158G01N33/57484G01N33/574G01N33/57492G01N33/48C07H21/04C12Q1/04
Inventor N·J·普雷斯曼K·S·赫施
Owner MONOGEN INC
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