Non-small cell lung cancer targeted therapy gene detection method

Abstract

The invention discloses a non-small cell lung cancer targeted therapy gene detection method, and belongs to the field of gene detection. The method for detecting 466 mutations of 12 oncogenes is developed by multiplex PCR and high throughput sequencing technologies, wherein the oncogenes are AKT1, ALK, BRAF, EGFR, ERBB4, FGFR1, FGFR2, FGFR3 , KRAS, MET, PIK3CA, and PTEN, and the mutations may be substitutions, insertions and / or deletions of one or more bases. The detection method provided by the invention has the advantages of high detection sensitivity of up to 0.01%, and clear and objective detection results, can be directly used for reflecting specific mutation sites of the relevant gene, directly used for guiding clinical non-small cell lung cancer targeted dosage, and used for early diagnosis or auxiliary diagnosis and screening of cancers as well as post-cancer surveillance.

Description

technical field [0001] The invention belongs to the field of gene detection, and more specifically relates to a gene detection method for non-small cell lung cancer targeted therapy. Background technique [0002] The third national survey on the causes of population death showed that in the past 30 years, the mortality rate of lung cancer in my country has increased by 465%, surpassing liver cancer to become the cancer with the highest mortality rate. It is estimated that in 2015, there were 733,300 new cases of lung cancer in my country (509,300 males and 224,000 females), and 610,200 deaths (432,400 males and 177,800 females), ranking first among all malignant tumors in terms of morbidity and mortality. . Therefore, lung cancer has become a very important public health problem in our country. [0003] Lung cancer can be divided into small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC) according to histology. Non-small cell lung cancer accounts for about 80...

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Problems solved by technology

[0011] In view of the existing non-small cell lung cancer detection methods that have problems such as difficult interpretation of results, poor repeatability, many false positives and false negatives, and low throughput, the present invention provides a non-small cell lung cancer targeted therapy gene detection method, which is a A method was developed based on multiplex PCR and high-throughput sequencing technology to detect 466 mutations in 12 oncogenes: AKT1, ALK, BRAF, EGFR, ERBB4, FGFR1, FGFR2, FGFR3, KRAS , MET, PIK3CA and PTEN, the mutations can be substitutions, insertions and / or deletions of one or more bases

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