64results about How to "Detection method safety" patented technology

The invention discloses a non-small cell lung cancer targeted therapy gene detection method, and belongs to the field of gene detection. The method for detecting 466 mutations of 12 oncogenes is developed by multiplex PCR and high throughput sequencing technologies, wherein the oncogenes are AKT1, ALK, BRAF, EGFR, ERBB4, FGFR1, FGFR2, FGFR3 , KRAS, MET, PIK3CA, and PTEN, and the mutations may be substitutions, insertions and / or deletions of one or more bases. The detection method provided by the invention has the advantages of high detection sensitivity of up to 0.01%, and clear and objective detection results, can be directly used for reflecting specific mutation sites of the relevant gene, directly used for guiding clinical non-small cell lung cancer targeted dosage, and used for early diagnosis or auxiliary diagnosis and screening of cancers as well as post-cancer surveillance.

The invention discloses a composite affinity column for purifying 3-acetyl deoxynivalenol, aflatoxin, ochratoxin A and zearalenone, a preparation method and an application thereof. According to the invention, by employing 4% of cylindrical agarose gel as a solid phase carrier, agarose gel and an antibody are coupled to form an immunoadsorbent, and filling in a column to prepare the immunization affinity column. When a sample containing 3-acetyl deoxynivalenol, aflatoxin, ochratoxin A and zearalenone passes through the immunization affinity column, 3-acetyl deoxynivalenol, aflatoxin, ochratoxin A and zearalenone can be specifically adsorbed by the immunoadsorbent, other impurities flows out of the immunization affinity column, then methanol is used for eluting 3-acetyl deoxynivalenol, aflatoxin, ochratoxin A and zearalenone from the column, so that the sample can be better purified.

The invention discloses a method for detecting fluidity of slag in a coal gasifier. The method comprises the following steps of: acquiring a sound wave signal; preprocessing the sound wave signal; extracting feature parameters of the sound wave signal; building a prediction model; and detecting the fluidity of the slag. The method for detecting the fluidity of the slag in the coal gasifier by combining an acoustic emission sensor or an acceleration sensor and a wave guiding rod has the characteristics of stability, safety, environmental friendliness and the like, and is applicable to on-line detection of an industrial production process; a sound wave receiving device array is adopted, and measurement accuracy is improved by multi-sensor data fusion; and on-line detection of the fluidity of the slag is realized by a slag fluidity index number detecting technology based on sound wave detection. Compared with the prior art, the method is sensitive and is high in detection accuracy; and early warning of slag blockage is realized by a slag blockage early warning and control technology based on slag fluidity index number detection, the viscosity of the slag can be controlled within a range above or below an objective value by 5%, and the slag blockage is effectively avoided.

The present invention relates to an immunoadsorbent and a composite affinity column for purification of fumonisin B1, aflatoxin, ochratoxin A and zearalenone. The immunoadsorbent comprises a solid carrier and fumonisin B1, aflatoxin, ochratoxin A and zearalenone monoclonal antibodies coupled with the solid carrier, the fumonisin B1 monoclonal antibody is secreted from hybridoma cell line Fm7A11 with the preservation number of CCTCCNO.C201636, the hybridoma cell line Fm7A11 is preserved in CCTCC in March 29, 2016 in Wuhan University Wuhan China. The composite affinity column is loaded with the immunoadsorbent with the fumonisin B1, aflatoxin, ochratoxin A and zearalenone monoclonal antibodies, and is used for purification of the fumonisin B1, aflatoxin, ochratoxin A and zearalenone. The composite affinity column can be used for purification and detection of fumonisin B1, aflatoxin, ochratoxin A and zearalenone toxin samples.

The embodiment of the invention discloses an elevator braking torque detection method and detection device. The method comprises the following steps that a data processing unit acquires detection data, wherein the detection data comprises a difference value (W1-W2) between an elevator counterweight weight W1 and a car weight W2, the torque M1 required at the static state of an elevator, torque Mxrequired at movement of an elevator car, a maximum acceleration value a1 of the elevator when a brake is opened in the power-off state of a traction machine, and a maximum acceleration value a2 of theelevator in deceleration stop process when the brake is closed; and the data processing unit judges whether the elevator braking torque is qualified or not according to the rated load capacity Q of the elevator and the detection data. Namely, the detection method used in the embodiment of the elevator braking torque detection method and the device is simple and safe, does not need to use a weight, and saves time and labor.

The invention discloses a kit for predicting pancreatic cancer patient prognosis adverse risks and an application thereof; CXCL12 and CXCR7 expression degrees in a tissue specimen of a pancreatic cancer patient are determined through an immunohistochemical method, CXCL12 and CXCR7 as markers can rapidly, safely, conveniently and effectively predict the pancreatic cancer patient prognosis adverse risks in in-vitro assessment of pancreatic cancer prognosis, and the basis is provided for clinical diagnosis and treatment.

The invention provides an antibody biochip for detecting H5 subtype avian influenza virus, its preparation method and application. The biochip provided by the present invention comprises a solid phase carrier, a gold film attached to the solid phase carrier, and an anti-H5N1 subtype avian influenza virus monoclonal antibody connected with the gold film, and the biochip is helpful for the detection of the H5 subtype The rapid and accurate detection and identification of avian influenza virus, and the quantitative analysis of H5 subtype avian influenza virus in the sample can truly reflect the advantages of specificity, sensitivity and rapidity of the biochip.

The invention discloses a supporting leg contracting state detecting method and device for a special vehicle. The method includes the steps of firstly, installing the detecting device; secondly, pushing a detecting rod to slide upwards through a collision block when a perpendicular movement leg contracts; thirdly, driving the detecting rod to move leftwards when a horizontal movement leg contracts; fourthly, triggering a detecting switch to send out a contracting state signal through the detecting rod when the perpendicular movement leg and the horizontal movement leg both reach the contracting positions; fifthly, sending out a disengagement signal through the detecting switch when any one supporting leg leaves the contracting position; sixthly, obtaining a vehicle driving state signal through a controller by means of a parking brake and a gear position, and starting an alarm device to give an alarm when the controller receives a supporting leg disengagement contracting state signal. By means of the detecting device, the contracting states of the perpendicular movement leg and the horizontal movement leg can be detected at the same time, the detecting and control principle is simple, no large electrical system or complicated electrical layout is needed, and cost and damage risk are reduced.

The invention discloses a method for quantitatively detecting hydroquinone by utilizing cobalt-based metal organic gel to catalyze luminiscence of Luminol-hydrogen peroxide. The method comprises the following steps: mixing the cobalt salt solution and dimethyl sulfoxide solution of 1,10-phenanthroline-2,9-dicarboxylic acid in equivalent amount to prepare the cobalt(II)-based metal organic gel, wherein the cobalt-based metal organic gel can catalyze the chemiluminiscence of the Luminol-hydrogen peroxide; taking hydroquinone as reducing agent, the hydrogen peroxide as the oxidant, thereby performing the redox reaction; mixing the hydroquinone and the hydrogen peroxide in a sampling value of an IFFM-E flow injection chemiluminiscence analyzer, and performing the reaction to reduce the hydrogen peroxide concentration, thereby reducing the chemiluminiscence strength. Along the gradually increasing of the concentration of the hydroquinone, the chemiluminiscence strength of the system is gradually reduced, and the quantitative detection of the hydroquinone can be realized by utilizing the gradual linear relation of the concentration of the hydroquinone and the chemiluminiscence strength.

The invention discloses a domestic pesticide residue detecting instrument and method, and the detecting instrument comprises a pesticide residue detection component, a heating component, a controller and a detection analysis component; the pesticide residue detection component comprises an acetylcholinesterase layer and a color developing layer, the acetylcholinesterase layer is provided with an encapsulation and acetylcholinesterase liquid in the encapsulation, the encapsulation is made with sodium alginate, the color developing layer is provided with a developer, the detection analysis component comprises an image acquisition unit and a color recognition unit, an input end of the image acquisition unit is connected with the color developing layer of the pesticide residue detection component, an output end of the image acquisition unit is connected with the color recognition unit, and an output end of the color recognition unit is connected with a signal acquisition end of the controller; a heating plate is a resistive heating plate; it is possible to solve the public concern on food safety, the detecting method is safe and reliable, is simple to perform and is suitable for any user.

The invention discloses a ring type polypeptide simulating antigenic epitope of aflatoxin B1 (AFB1), and a detection kit of the AFB1. The amino acid sequence of the ring type polypeptide is CVPSKPGLC,which is shown as SEQ ID No.1. The kit comprises an AFB1 competitive antigen, an elisa plate embedded with an anti-AFB1 monoclonal antibody, a standard solution of the aflatoxin B1, horseradish peroxidase labeled streptavidin and the like. The polypeptide sequence can simulate the antigenic epitope of the AFB1, can be subjected to specific binding with an antibody for screening, and is high in sensitivity and high in specificity, exposure of the AFB1 to operators is reduced, and the safer and the more efficient detection method is achieved. The ring type polypeptide simulating the antigenic epitope of the AFB1, and the detection kit of the AFB1 have the significant practical significance for achieving pollution monitoring of the AFB1 in agricultural products.

The invention relates to a molecular probe and a kit for early prediction of metastatic prostate cancer and an application of the molecular probe. The molecular probe for early prediction of the metastatic prostate cancer comprises an LCRMP4 probe, and the sequence of the LCRMP4 probe is as shown in SEQ ID NO: 8. The kit for early prediction of the metastatic prostate cancer comprises the molecular probe. When the molecular probe and the kit are used for early prediction of the metastatic prostate cancer, accuracy, sensitivity and specificity are high.

The invention provides a jack-up type detection method and apparatus for pulling-off force of magnetic hoisting equipment. The jack-up type detection method for pulling-off force of magnetic hoisting equipment is characterized by disposing detected magnetic hoisting equipment on a platform of a jack-up type pulling-off force detection apparatus to enable the magnetic hoisting equipment to be in the absorbed state and to be absorbed to the surface of the platform with a plurality of through holes; setting a push rod in each through hole in the surface of the platform; using the push rods to jack up the magnetic hoisting equipment from bottom to top to enable the magnetic hoisting equipment to separate from the surface of the platform; and as the jacking force is generated at the moment that the magnetic hoisting equipment is separated from the surface of the platform, being able to measuring the pulling-off force of the magnetic hoisting equipment. The jack-up type detection apparatus for pulling-off force of magnetic hoisting equipment is characterized in that the top of the detection apparatus is a platform steel plate, and the bottom of the detection apparatus is a box body; the box body is formed by a side plate, an end plate, a base plate and a foot disk; a teeth bed is arranged in the box body, and is formed by an orifice plate, a supporting plate, reinforcing rib plates and push rods; pressure sensors are arranged below the teeth bed; each hydraulic jack is arranged below each pressure sensor; a hand pump and a display screen are arranged outside the box body; the hand pump and the hydraulic jacks are connected with each other through a pipeline; and the display screen is connected with the pressure sensors through a lead.

The present invention provides a micro-invasive intracranial pressure detection system and its application method, belonging to the field of craniocerebral detection technology. Said system includes the following several portions: sensor, electric bridge measurement circuit, signal amplification circuit, phase-sensitive detection circuit, filter circuit, data acquisition circuit, strain correction system, calvaria and dura mater encephalic viscoelasticity processing system, result display, oscillating circuit and power supply, in which the strain correction system and calvaria and dura mater encephalic viscoelasticity processing system are formed into a microcomputer processing system. Besides, said invention also provides the concrete steps of its application method.

The invention relates to a method for detecting residual copper layer distribution of a printed circuit board, and belongs to the technical field of chemical engineering. The method comprises the steps that: an auxiliary solution containing stannous chloride and a detection solution containing palladium chloride are adopted to act with the etched copper layer, copper dots or copper seed in sequence, so that an observed black product can be formed, wherein the auxiliary solution is used for enabling divalent tin ions to permeate and be adsorbed on a residual copper layer, copper dots or copperteeth, palladium ions in the detection solution can be reduced into black dot palladium metal by the divalent tin ions adsorbed on the copper and the copper, and the black dot palladium metal can be obviously observed by naked eyes, so that the distribution of the residual copper is detected. The detection method provided by the invention is simple, convenient, accurate and safe, and can meet thesurface quality detection requirement in the production process of the printed circuit board.

The invention provides a film discharge structure and a detection method used for detecting a reinforcement effect in vacuum prepressing. The detection method comprises that a film discharge tube with a diameter of no less than 90 mm and a length of no less than 1300 mm is prepared at first and then laid on a drainage sand cushion layer, the film discharge tube is embedded into a soft soil layer to be reinforced, a lower-layer geotextile equipped with a film discharge hole and a vacuum film are then laid successively, a rolled edge turned upwards is preserved on the edge of the film discharge hole, the rolled edge is sealed and fixed on the film discharge tube by a PVC adhesive and an elastic binding role, the vacuum prepressing is then carried out for a certain period, a detection probing rod of a detection device at an original position is inserted into the soft soil layer to be reinforced through an upper tube hole of the film discharge tube to realize original position detection, and secondary sealing is carried out to a tube hole of the film discharge tube after the detection. The film discharge structure and the detection method used for detecting the reinforcement effect in the vacuum prepressing provided by the invention satisfy requirements for vacuum degree stabilization under a film and continuous loading and prepressing, and can also satisfy requirements for detecting the soft soil reinforcement effect during the vacuum prepressing or vacuum combined preloading.

The invention belongs to the field of medical science detection, and especially relates to a kit for detecting versican V1mRNA in urine, and an application thereof. The urine of a patient is collected, and the versican V1mRNA level of urinary sediment is determined. A result shows that the versican V1mRNA level of the urinary sediment of an FSGS patient is significantly increased, and the versicanV1mRNA level of the urinary sediment of a minimal change disease (MCD) patient has no change; and the versican V1mRNA level of the urinary sediment of the FSGS patient is associated with the tubulointerstitial fibrosis score of the patient, and is significantly associated with the decline rate of eGFR during patient follow-up, so the expression level of the versican V1mRNA in urine can be used for the prognostic diagnosis of the FSGS patient. A reagent for detecting the versican V1mRNA expression level of urine can be used to prepare a detection reagent or the detection kit in order to realize the non-invasive diagnosis of the FSGS prognosis.

The invention discloses a kit for aided identification of Plum Pox virus and application thereof. The kit provided by the invention comprises a special primer pair and/or a special probe, wherein the special primer pair is the primer pair consisting of the DNAs presented by sequence 1 and sequence 2; and the nucleotide sequence of the special probe is presented by sequence 3. The kit of the invention can quickly, detect the Plum Pox virus accurately, stably and quantitatively, can judge whether a sample contains the Plum Pox virus or not according to the fluorescent signal generation condition, and can judge whether the content of the Plum Pox virus in the sample is high or low according to the size of the signal value. The kit has the advantages of strong specificity, high accuracy, simple and convenient operation in a detection process, short time required by detection and the like, and is suitable for quarantine inspection stations, port inspection and quarantine bureaus, plant virus research institutions and the like of agriculture department.

The invention belongs to the technical field of medical instruments, and discloses an intelligent puncture needle, which comprises a needle tube, a needle head is fixedly mounted at the left end of the needle tube, a wire is movably connected to an inner cavity of the needle tube, an induction area is arranged on the left side face of the needle head, and an induction circuit is fixedly connected to the interior of the induction area. A control table is electrically connected to the right end of the wire, a data acquisition chip is fixedly installed in the control table, a data analyzer is electrically connected to the rear end of the data acquisition chip, a displayer is fixedly installed at the rear end of the data analyzer, and a sealing piece is fixedly installed in the needle tube. By designing the needle head and the induction electrode, when an infant is treated, puncture on the lumbar vertebra and spinal fluid of the infant can be accelerated, pains of the infant are reduced, various dangers are eliminated, meanwhile, when an adult is punctured, tumor detection is more accurate and efficient, and the product is worthy of popularization.

The invention belongs to the technical field of chemical detection, and particularly relates to a method for rapidly detecting the hydroxyl radical scavenging rate of health care wine, which comprises the following steps: preparing a rhodamine B solution, a FeSO4 solution, a H2O2 solution and an ascorbic acid solution; ascorbic acid is used as a standard hydroxyl free radical scavenger, and a standard color developing card is manufactured; calibrating a standard hydroxyl free radical scavenging rate value by using an ultraviolet-visible spectrophotometric method; and testing the to-be-tested health-care wine sample, and determining the hydroxyl radical scavenging rate value of the health-care wine. According to the invention, ascorbic acid is used as a standard to make the standard color developing card, so that the oxidation resistance of the health-care wine has relatively strong reference and comparability, and the detection method has the advantages of rapidness, convenience, simplicity, safety and low cost.