Molecular probe and kit for early prediction of metastatic prostate cancer and application of molecular probe

A technology of prostate cancer and molecular probes, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of low specificity and sensitivity, hysteresis, and inability to be used repeatedly for a long time etc. to achieve the effects of high sensitivity and specificity, less trauma, and automated detection methods

Active Publication Date: 2017-03-08
广州立柏生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The above detection methods for prostate cancer have the following defects: Common imaging methods, by obtaining anatomical information and metabolic information, assess the size of the tumor (tumor lesions >0.2cm) and tumor metabolism, and then judge the current status of the tumor. However, it often needs to wait until the tumor has a certain volume before it can be discovered. Therefore, there is a certain lag. In addition, because of the safety of radiation, it cannot be used repeatedly for a long time
In terms of serology, it reflects the state of the tumor through tumor protein markers, so the specificity and sensitivity are still low
As for the pathological method, it can accurately obtain the genetic information of the tumor, and then it relies on invasive tumor tissue samples, because there are certain limitations for patients who have no way to obtain tissue samples at the advanced stage

Method used

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  • Molecular probe and kit for early prediction of metastatic prostate cancer and application of molecular probe
  • Molecular probe and kit for early prediction of metastatic prostate cancer and application of molecular probe
  • Molecular probe and kit for early prediction of metastatic prostate cancer and application of molecular probe

Examples

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Effect test

Embodiment 1

[0041] An embodiment of the probe set for early prediction of metastatic prostate cancer in the present invention, the probe set for early prediction of metastatic prostate cancer described in this embodiment includes epithelial markers, mesenchymal markers, CD45 probes and LCRMP4 probes ; The epithelial marker is made up of EpCAM probe, CK8 probe, CK18 probe and CK19 probe, and the mesenchymal marker is made up of Vimentin probe and Twist probe; Wherein, the sequence of the EpCAM probe is as follows Shown in SEQ ID NO:1, the sequence of the CK8 probe is shown in SEQ ID NO:2, the sequence of the CK18 probe is shown in SEQ ID NO:3, the sequence of the CK19 probe is shown in SEQ ID NO:4, Vimentin The sequence of the probe is shown in SEQ ID NO:5, the sequence of the Twist probe is shown in SEQ ID NO:6, the sequence of the CD45 probe is shown in SEQ ID NO:7, and the sequence of the LCRMP4 probe is shown in SEQ ID NO :8 shown. The specific sequences of the probes are shown in Tab...

Embodiment 2

[0046] An embodiment of the kit for early prediction of metastatic prostate cancer of the present invention, the kit for early prediction of metastatic prostate cancer described in this embodiment is an in situ hybridization detection kit, which includes: hybridization probes, permeabilization Reagent, digestion solution, hybridization buffer solution, pre-amplification working solution, amplification working solution, chromogenic solution, counterstaining solution and washing solution;

[0047]Wherein, the hybridization probe is the probe set described in Example 1, the LCRMP4 probe, EpCAM probe, CK8 probe, CK18 probe, CK19 probe, Vimentin probe, Twist probe and CD45 probe Both have markers; epithelial markers (EpCAM probe, CK8 probe, CK18 probe and CK19 probe) are labeled with red fluorescence; mesenchymal markers (Vimentin probe and Twist probe) are labeled with green fluorescence; The LCRMP4 probe is labeled with purple fluorescence; the CD45 probe is labeled with blue flu...

Embodiment 3

[0055] In this example, the kit described in Example 2 was used to detect the expression level of LCRMP4 by using the RNA in situ hybridization technique of CTCs to predict metastatic prostate cancer. In this example, the process of detecting the expression level of LCRMP4 by using the RNA in situ hybridization technique of CTCs is as follows figure 1 shown.

[0056] The specific steps are as follows:

[0057] 1. Clinical sample collection and processing

[0058] a. Collect peripheral blood:

[0059] 1. Collect 5 mL of peripheral blood with EDTA anticoagulant blood collection tubes, and collect 2 tubes (10 mL for each patient);

[0060] 2. After blood collection, invert the blood collection tube more than 5 times and mix well, then store it at 4°C, and the storage time should not exceed 4 hours;

[0061] 3. Record the corresponding number of the patient, and use the connector to transfer the blood in the blood collection tube into the sample storage tube (containing red bl...

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Abstract

The invention relates to a molecular probe and a kit for early prediction of metastatic prostate cancer and an application of the molecular probe. The molecular probe for early prediction of the metastatic prostate cancer comprises an LCRMP4 probe, and the sequence of the LCRMP4 probe is as shown in SEQ ID NO: 8. The kit for early prediction of the metastatic prostate cancer comprises the molecular probe. When the molecular probe and the kit are used for early prediction of the metastatic prostate cancer, accuracy, sensitivity and specificity are high.

Description

technical field [0001] The present invention relates to a molecular probe, a kit and the application of the molecular probe, in particular to a molecular probe for the early prediction of metastatic prostate cancer, the kit and the application of the molecular probe. Background technique [0002] Prostate cancer (PCa) is one of the malignant tumors that seriously threaten men's health. Its incidence rate ranks first in European and American countries, and its mortality rate ranks second. In recent years, with my country's economic development, population aging and dietary changes, the incidence and mortality of PCa have been increasing year by year. The latest cancer statistics in 2015 show that PCa has become the malignant tumor with the highest morbidity and mortality rate and the fastest growth rate in the male reproductive system of the genitourinary system. The 5-year survival rate of patients with metastatic prostate cancer is only 34%. PCa is rapidly affecting the qu...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6841C12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/16C12Q2537/143
Inventor 高新庞俊陈征
Owner 广州立柏生物技术有限公司
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