Kit for predicting pancreatic cancer patient prognosis adverse risks and application thereof

A technology for poor prognosis and pancreatic cancer, applied in the field of medicine and biology, can solve the problems of evaluating the prognosis of patients with less pancreatic cancer, lack of effective methods and products to evaluate the prognosis of pancreatic cancer patients, etc., and achieve the effect of easy promotion.

Inactive Publication Date: 2015-12-09
PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although the research on the treatment of pancreatic cancer is in full swing, few studies focus on the evaluation of the prognosis of pancreatic cancer patients, and there are no effective methods and products to evaluate the prognosis of pancreatic cancer patients

Method used

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  • Kit for predicting pancreatic cancer patient prognosis adverse risks and application thereof
  • Kit for predicting pancreatic cancer patient prognosis adverse risks and application thereof
  • Kit for predicting pancreatic cancer patient prognosis adverse risks and application thereof

Examples

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Embodiment 1

[0022] Embodiment 1: Utilize the kit of the present invention to pancreatic cancer tissue (specimen is derived from the cancer tissue of surgical resection of 161 patients with pancreatic cancer, wherein 104 are male patients, 57 are female patients, the age span is 34-85 years old, the median 59 years old) expressed chemokine CXCL12 and chemokine receptor CXCR7 for detection

[0023] The pancreatic cancer prognosis kit in this embodiment consists of the following components:

[0024] (1) Reagent A: blocking solution, which is 10% goat serum;

[0025] (2) Reagent B: diluted ready-to-use anti-CXCL12 primary antibody;

[0026] (3) Reagent C: diluted ready-to-use anti-CXCR7 primary antibody;

[0027] (4) Reagent D: anti-goat biotinylated secondary antibody;

[0028] (5) Reagent E: streptavidin-labeled HRP;

[0029] (6) Reagent F: 20 times concentrated DAB substrate solution;

[0030] (7) Reagent G: 20 times concentrated DAB substrate buffer solution;

[0031] (8) Reagent H:...

Embodiment 2

[0067] Example 2 Application of the kit of the present invention in predicting the prognosis of pancreatic cancer patients

[0068] Using the case-control research method and using kits produced in small experiments, the expression of chemokine CXCL12 and chemokine receptor CXCR7 in the tissue samples of 161 patients with pancreatic cancer who underwent radical surgery were detected, and the expression of chemokine receptor CXCR7 was detected. Long-term follow-up of cancer patients (monthly telephone follow-up, follow-up for 87 months, no follow-up if the patient dies during the period), using statistical methods to study the chemokine CXCL12 and chemokine receptors in pancreatic cancer tissue The relationship between the intensity of CXCR7 expression and the prognosis of patients. like figure 2 As shown, the median survival time of CXCL12 and CXCR7 common high expression group was significantly shorter than CXCL12 and CXCR7 common low expression group and CXCL12 or CXCR7 hi...

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Abstract

The invention discloses a kit for predicting pancreatic cancer patient prognosis adverse risks and an application thereof; CXCL12 and CXCR7 expression degrees in a tissue specimen of a pancreatic cancer patient are determined through an immunohistochemical method, CXCL12 and CXCR7 as markers can rapidly, safely, conveniently and effectively predict the pancreatic cancer patient prognosis adverse risks in in-vitro assessment of pancreatic cancer prognosis, and the basis is provided for clinical diagnosis and treatment.

Description

technical field [0001] The invention belongs to the field of medical biotechnology, and relates to a kit for predicting the risk of poor prognosis of pancreatic cancer patients by measuring the expression strength of chemokine CXCL12 and chemokine receptor CXCR7 in pancreatic cancer tissue samples and its application. Background technique [0002] Pancreatic cancer is a malignant tumor of the digestive tract that seriously threatens human health. Its clinical manifestations are hidden, the disease progresses rapidly, and the prognosis is extremely poor. 85% of the patients are already in the advanced stage when they are diagnosed, only about 20% of the patients can be treated surgically, and the overall 5-year survival rate is less than 5%. Therefore, it is called "the stubborn fortress of medicine in the 21st century" internationally. In recent years, the incidence of pancreatic cancer has been increasing year by year worldwide. In western countries, pancreatic cancer has ...

Claims

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Application Information

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IPC IPC(8): G01N33/574
CPCG01N33/57438
Inventor 郭俊超赵玉沛张太平周立李建由磊
Owner PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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