Rapid detection method for hydroxyl radical scavenging rate of health-care wine

A detection method, free radical technology, applied in the field of chemical detection, can solve problems such as limitations, complicated procedures, long time, etc., and achieve the effect of low cost, strong reference and comparability

Pending Publication Date: 2022-06-03
SHANXI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, how to quickly and effectively evaluate the antioxidant activity of health wine is still a challenge
At present, the commonly used methods for determining the scavenging rate of hydroxyl radicals include spectrophotometry, chemiluminescence, electron spin resonance, high-performance liquid chromatography, etc. These methods consume more time, use expensive instruments, and have more complicated procedures. factors, which are limited in practical applications

Method used

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  • Rapid detection method for hydroxyl radical scavenging rate of health-care wine
  • Rapid detection method for hydroxyl radical scavenging rate of health-care wine
  • Rapid detection method for hydroxyl radical scavenging rate of health-care wine

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Embodiment

[0032] A rapid detection method for alcohol hydroxyl radical scavenging rate, comprising the following steps:

[0033] Step 1: Preparation of stock solution;

[0034] Preparation 7.00×10 -3 mol / L Rhodamine B solution: Accurately weigh 0.80-0.85g of Rhodamine B in a small beaker, add an appropriate amount of deionized water to dissolve, transfer to a 250.00mL volumetric flask, and make up to the mark with deionized water to obtain a concentration of 7.00×10 -3 mol / L Rhodamine B solution;

[0035] Prepare 0.1mol / L FeSO 4 Solution: accurately weigh FeSO 4 ·7H 2 O 0.60-0.70g in a small beaker, add an appropriate amount of deionized water to dissolve, and add 2ml of 0.5mol / L dilute H 2 SO 4 The solution was transferred to a 25.00mL volumetric flask, and the volume was adjusted to the mark with deionized water to obtain a concentration of 0.1mol / L FeSO 4 solution.

[0036] Prepare 0.1mol / L H 2 O 2 Solution: accurately pipette H 2 O 2 Put 250-260μL of the solution in a 25....

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Abstract

The invention belongs to the technical field of chemical detection, and particularly relates to a method for rapidly detecting the hydroxyl radical scavenging rate of health care wine, which comprises the following steps: preparing a rhodamine B solution, a FeSO4 solution, a H2O2 solution and an ascorbic acid solution; ascorbic acid is used as a standard hydroxyl free radical scavenger, and a standard color developing card is manufactured; calibrating a standard hydroxyl free radical scavenging rate value by using an ultraviolet-visible spectrophotometric method; and testing the to-be-tested health-care wine sample, and determining the hydroxyl radical scavenging rate value of the health-care wine. According to the invention, ascorbic acid is used as a standard to make the standard color developing card, so that the oxidation resistance of the health-care wine has relatively strong reference and comparability, and the detection method has the advantages of rapidness, convenience, simplicity, safety and low cost.

Description

technical field [0001] The invention belongs to the technical field of chemical detection, and in particular relates to a rapid detection method for the scavenging rate of hydroxyl radicals of health-care wine. Background technique [0002] Hydroxyl radical ( OH) is considered to be the most active reactive oxygen species produced by human metabolism, and its half-life in the body is about 10. -9 seconds, has a strong oxidizing ability. Excess OH can interact with molecules in the organism through addition reactions, electron transfer, dehydrogenation and other methods, resulting in oxidative damage to lipids, nucleic acids and other substances, and can also cause protein denaturation and enzyme inactivation. Eventually lead to the aging of the body or cause cancer, diabetes and many other diseases. ·OH scavenger can inhibit the damage produced by ·OH and maintain the normal life metabolism of the body. Effective OH scavengers are of practical significance for preventing ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/01
CPCG01N21/78G01N21/01G01N2021/0112Y02A50/30
Inventor 王英特张媛媛吴蓉蓉王丽苹王云霞
Owner SHANXI UNIV
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