Rapid detection method for hydroxyl radical scavenging rate of health-care wine
A detection method, free radical technology, applied in the field of chemical detection, can solve problems such as limitations, complicated procedures, long time, etc., and achieve the effect of low cost, strong reference and comparability
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[0032] A rapid detection method for alcohol hydroxyl radical scavenging rate, comprising the following steps:
[0033] Step 1: Preparation of stock solution;
[0034] Preparation 7.00×10 -3 mol / L Rhodamine B solution: Accurately weigh 0.80-0.85g of Rhodamine B in a small beaker, add an appropriate amount of deionized water to dissolve, transfer to a 250.00mL volumetric flask, and make up to the mark with deionized water to obtain a concentration of 7.00×10 -3 mol / L Rhodamine B solution;
[0035] Prepare 0.1mol / L FeSO 4 Solution: accurately weigh FeSO 4 ·7H 2 O 0.60-0.70g in a small beaker, add an appropriate amount of deionized water to dissolve, and add 2ml of 0.5mol / L dilute H 2 SO 4 The solution was transferred to a 25.00mL volumetric flask, and the volume was adjusted to the mark with deionized water to obtain a concentration of 0.1mol / L FeSO 4 solution.
[0036] Prepare 0.1mol / L H 2 O 2 Solution: accurately pipette H 2 O 2 Put 250-260μL of the solution in a 25....
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