Anti-interference rapid detection and reagent for microbe
A detection method and microbial technology, applied in the direction of chemiluminescence/bioluminescence, analysis of materials through chemical reactions, bacteria, etc., can solve the problems of interference from preservatives or residual disinfectants, insufficient sensitivity, etc., to shorten the detection time, Effect of Improving Detection Sensitivity
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Embodiment 1
[0058] Embodiment 1: Bacteria liquid large sample rapid determination in bottled drinking natural mineral water
[0059] Because bottled drinking natural mineral water is usually disinfected with ozone, an anti-ozone bacterial liquid large-sample detection medium is selected:
[0060] a. Preparation of culture medium: prepare 50mL / bottle of three-material liquid culture medium according to the instruction of anti-ozone bacteria liquid large sample detection medium, divide into 250mL triangular flasks, prepare other supporting supplies, and then sterilize;
[0061] b. Cultivation of microorganisms: In a 100-level ultra-clean workbench, add 100mL of the sample to be tested into a 250mL Erlenmeyer flask filled with cooled 50mL / bottle of three-material medium, shake and mix, and then culture at 35-37°C 6h;
[0062] c. Microbial cell ATP extraction method: take 10mL of culture medium, centrifuge at 10000r / min for 5 minutes, remove the supernatant, add 1mL of microbial cell ATP rel...
example 2
[0068] Example 2: Rapid Determination of MPN Method for Yeast and Mold in Juice Drinks
[0069] Juice drinks are usually added with preservatives to prolong the shelf life, but the freshly produced products contain very low bacteria, so to determine the microbial count of samples with low bacterial counts containing preservatives, the MPN method can be used. ), to test the sample:
[0070] a. Preparation of culture medium: according to the national standard coliform number multi-tube fermentation method (9-tube method), prepare anti-corrosion agent-type fungal liquid large sample detection medium (no need to add a small inverted tube to the test tube), 10mL per tube, and prepare Other supporting supplies, and then sterilized;
[0071] b. Cultivation of microorganisms: In a 100-class ultra-clean workbench, add the mixed and dispersed samples into the measurement system according to the multi-tube fermentation method for the number of coliforms in the national standard, shake a...
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