Method and kit for detecting lung cancer susceptibility gene

Abstract

The invention relates to the field of genetic engineering, and provides a method and a corresponding kit for detecting a lung cancer susceptibility gene. The method for detecting the lung cancer susceptibility gene comprises the following steps of: A, using a specificity primer of the lung cancer susceptibility gene to amplify a plurality of target areas in a sample to be detected, and establishing a sequencing library based on amplified products; B, subjecting the sequencing library to monomolecular amplification to obtain a plurality of monomolecular amplification products corresponding to the plurality of target areas; and C, subjecting the plurality of monomolecular amplification products to high-throughput gene sequencing at the same time so as to obtain sequence information of the plurality of target areas. The method and the corresponding kit are used for sequencing the plurality of areas of the lung cancer susceptibility gene at the same time by using a high-throughput sequencing technology; the sequence information including variation of known mutations and unknown mutations in the areas can be obtained accurately; the kit has high detection sensitivity, and can be used for further detecting mainly samples at the same time.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a method and kit for detecting lung cancer susceptibility genes. Background technique [0002] Susceptibility genes refer to genes or alleles that have a certain association with a specific disease. Detect the susceptibility genes of complex diseases to obtain their specific sequence information, and then combine further molecular biology tests, clinical trials, clinical observations and statistical analysis of comprehensive data to establish a certain disease model, which can realize early evaluation of diseases susceptibility and take corresponding preventive measures to reduce the probability of disease occurrence. A large number of studies have confirmed that genetic factors play an important role in the susceptibility of diseases, and the genetic factors of complex diseases have been confirmed to have a minor gene dosage effect, that is, the effects of multiple minor gene...

AI Technical Summary

Problems solved by technology

At present, the most common method for detecting lung cancer susceptibility genes is Sanger sequencing, which can detect regions of lung cancer susceptibility genes, but Sanger sequencing can only sequence a certain region of a sample at a time, and the sequencing cost is high , the sensitivity is low (20%), and the precise data of the variation of each mutation site cannot be obtained

[0005] In addition, although there have been many research results on the detection of lung cancer susceptibility genes and SNP sites, the existing technology is suitable for the detection and analysis of a single SNP site, and is not suitable for joint detection and analysis of multiple SNP sites. Not accurate enough

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