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A sandwich-type electrochemical sensor for detection of ovarian skov-3 cancer cells

An electrochemical and ovarian cancer technology applied in the field of materials to achieve high sensitivity and high selectivity

Inactive Publication Date: 2011-12-21
SHANGHAI NORMAL UNIVERSITY
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  • Abstract
  • Description
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Problems solved by technology

Compared to immuno-polymerase chain reaction, there are few reports on immunosensors for DNA-labeled antibodies to detect cancer cells.

Method used

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  • A sandwich-type electrochemical sensor for detection of ovarian skov-3 cancer cells
  • A sandwich-type electrochemical sensor for detection of ovarian skov-3 cancer cells
  • A sandwich-type electrochemical sensor for detection of ovarian skov-3 cancer cells

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Experimental program
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Embodiment 1

[0028] Such as figure 1 Steps A and B are shown to prepare the immunoelectrochemical sensor of the present invention.

[0029] (1) Preparation of ssDNA-anti-HER2 biological conjugate (probe DNA oligonucleotide-anti-HER2 biological conjugate)

[0030] First, add 0.5 μg / μL anti-HER2 antibody to the solution containing 2.12×10 -6 mol / L probe DNA oligonucleotide (ssDNA), 0.1mol / L 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and 0.1mol / L N-hydroxy In a 10 mmol / L phosphate buffer solution (pH 7.3) of sulfosuccinimide (NHSS), the reaction was shaken at room temperature for 3 hours. The complex was purified by high performance liquid chromatography. The mobile phase is a standard phosphate buffer solution (0.2mol / L, pH 7.3), the flow rate is 1ml / min, and the first peaked substance is the ssDNA-anti-HER2 biological conjugate. Finally, a spectrophotometer is used to qualitatively detect the ssDNA-anti-HER2 biological conjugates that flow out of the HPLC.

[003...

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Abstract

The invention belongs to the technical field of analytical chemistry and a chemical sensor, and discloses an electrochemical sensor for diagnosis of an ovarian SKOV-3 cancer cell, relating to a preparation method of a sandwich-type immunosensor based on graphene and a DNA (deoxyribonucleic acid) marker and application of the same in medical diagnosis. Antigens (human ovarian cancer) are indirectly tested with a glassy carbon electrode modified by graphene / antibody / antigen / DNA-labeled antibody / complementary DNA through catalytic oxidation based on guanine bases and adenine bases. The high electronic conductivity of the graphene is adopted, and the cost of the antibody marker is reduced. The immunosensor prepared based on the technical scheme can also be applied in detection of other types of cells has a wide scope of application. The sensor has high sensitivity, can be prepared simply, can be applied in detection any cancer cell, and has good prospects of application in medical diagnosis.

Description

technical field [0001] The invention relates to the field of materials, medical diagnosis and sensing technology, in particular to an electrochemical sensor for measuring ovarian cancer SKOV-3 cells. Background technique [0002] Malignant tumors are a huge threat to human health, and ovarian cancer is a malignant tumor that occurs in ovarian tissue. It can be seen that the detection of the level of ovarian cancer cells is of great significance. Electroanalytical chemistry is widely used in the electrochemical detection of living substances due to its advantages of high sensitivity, good selectivity, short response time, and low cost of required instruments and equipment. In the past, some tumor detection methods were mainly based on immunological methods, which were high in cost, complicated in operation steps, and prone to false negative results, which were the fatal shortcomings of this method. Therefore, the development of high sensitivity (for the detection of trace S...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/30G01N33/574
Inventor 黄杉生卜扬
Owner SHANGHAI NORMAL UNIVERSITY
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