Prussian blue nanometer particles for targeting beta age spots and preparation method of prussian blue nanometer particles

A Prussian blue and nanoparticle technology, applied in the field of biomedicine, can solve the problem that Prussian blue nanoparticles are difficult to be modified in a targeted manner, and achieve good biocompatibility and biosafety, simple operation and good stability.

Active Publication Date: 2019-07-23
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Prussian blue nanoparticles are difficult to carry out targeted modification, and it is difficult to accurately aggregate to the lesion site, and most of the synthesized particles are mostly used for tumor diagnosis

Method used

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  • Prussian blue nanometer particles for targeting beta age spots and preparation method of prussian blue nanometer particles
  • Prussian blue nanometer particles for targeting beta age spots and preparation method of prussian blue nanometer particles
  • Prussian blue nanometer particles for targeting beta age spots and preparation method of prussian blue nanometer particles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1) Dissolve 0.01 mmol of potassium ferrocyanide and 0.001 mmol of mPEG-COOH in 5 ml of deionized water, and mix thoroughly to obtain a clear solution A; dissolve 0.01 mmol of ferric chloride in 5 ml of deionized water, Fully dissolve to obtain a clear solution B; add solution B dropwise to solution A, and react at 60°C for 0.5h;

[0031] 2) Wait until the reaction system is lowered to room temperature, react for 0.5 h, and centrifuge to wash to obtain a precipitate C of Prussian blue nanoparticles modified with polyethylene glycol containing carboxyl groups;

[0032] 3) Precipitate C, 0.1 mmol of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride were dissolved in 10 ml of carboxyl-containing polyethylene glycol-modified Prussian blue nanoparticles In deionized water, react at room temperature for 15 minutes to obtain solution D;

[0033] 4) Add 0.1 mmol N-hydroxysuccinimide, 0.01 mg transferrin, and 0.4 micromol Congo red to solution D, react at room temperat...

Embodiment 2

[0036] 1) Dissolve 0.005 mmol of potassium ferrocyanide and 0.005 mmol of mPEG-COOH in 5 ml of deionized water respectively, and mix well to obtain a clear solution A; dissolve 0.005 mmol of ferric chloride in 5 ml of deionized water, Fully dissolve to obtain a clear solution B; add solution B dropwise to solution A, and react at 55°C for 0.5h;

[0037] 2) After the reaction system is lowered to room temperature, react for 1 hour, and centrifuge to wash to obtain a precipitate C of Prussian blue nanoparticles modified with polyethylene glycol containing carboxyl groups;

[0038] 3) Precipitate C, 0.05 mmol of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride were dissolved in 10 ml of carboxyl-containing polyethylene glycol-modified Prussian blue nanoparticles In deionized water, react at room temperature for 10 minutes to obtain solution D;

[0039] 4) Add 0.04 mmol N-hydroxysuccinimide, 0.005 mg transferrin, and 0.48 micromol Congo red to solution D, react at room...

Embodiment 3

[0042] 1) Dissolve 0.0195 mmol of potassium ferrocyanide and 0.003 mmol of COOH-PEG-COOH in 5 ml of deionized water, and mix well to obtain clear solution A; dissolve 0.0195 mmol of ferric chloride in 5 ml of deionized water Fully dissolve in water to obtain a clear solution B; add solution B dropwise to solution A, and react at 60°C for 1 hour;

[0043] 2) Wait until the reaction system is lowered to room temperature, react for 0.5 h, and centrifuge to wash to obtain a precipitate C of Prussian blue nanoparticles modified with polyethylene glycol containing carboxyl groups;

[0044] 3) Precipitation C and 0.12 mmol of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride were dissolved in 10 ml of carboxyl-containing polyethylene glycol-modified Prussian blue nanoparticles In deionized water, react at room temperature for 25 minutes to obtain solution D;

[0045] 4) Add 0.02 mmol of N-hydroxysuccinimide, 0.02 mg of transferrin, and 0.6 micromol of Congo red to solution...

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Abstract

The invention relates to a nanometer material belonging to the field of biomedical science, in particular to a preparation method of prussian blue nanometer particles for targeting A beta age spots. The prussian blue nanometer particles are functionally modified, so that the prussian blue nanometer particles can load transferrin having the function of penetrating through a blood-brain barrier andcongo red molecules targeting A beta age spots. The prepared nanometer particles have favorable r1 longitudinal relaxation rate and r2 lateral relaxation rate, so that simultaneous imaging of MRI(T1 / T2) of the A beta age spots can be realized.

Description

technical field [0001] The invention relates to nanomaterials in the field of biomedicine, in particular to a Prussian blue nanoparticle targeting Aβ senile plaques and a preparation method thereof. Background technique [0002] Alzheimer's disease (AD) is a neurodegenerative disease, the main clinical manifestations are memory and cognitive decline, daily life ability decline, etc., the typical pathological feature is β-amyloid (amyloidβpeptide, Aβ) Deposited extracellularly, intracellularly hyperphosphorylated tau protein to form neurofibrillary tangles and neuronal loss. Among them, senile plaques caused by Aβ deposition can cause a series of lesions in brain tissue, such as inflammation, oxidative stress, mitochondrial dysfunction, etc., play an important role in the course of AD, and are generally considered to be ideal targets for AD diagnosis. MRI has the advantages of no radioactivity, high spatial resolution, and a wide range of applications. However, existing MRI ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/14A61K49/10A61K49/18
CPCA61K49/10A61K49/14A61K49/1833A61K49/1866
Inventor 常津赵冬菊窦妍
Owner TIANJIN UNIV
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