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Protein biological template-based gadolinium-doped copper sulfide nano-particles and preparation method thereof

A technology of nanoparticles and biological templates, which can be applied in preparations for in vivo experiments, medical preparations containing active ingredients, drug combinations, etc., can solve the problems of the biological safety of the surface ligands of copper sulfide nanoparticles to be considered, etc. Achieve the effects of convenient mass production, high relaxation efficiency, and easy concentration and collection

Inactive Publication Date: 2016-10-12
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the preparation of copper sulfide nanoparticles mainly includes high-temperature pyrolysis method and aqueous phase controlled nucleation method. The former not only requires the participation of organic solvents, but also needs to be prepared at high temperature (180 ° C) and in an inert gas atmosphere. Water-based modification can be used for subsequent biomedical applications; the latter generally requires relatively high temperatures (90°C), and the biological safety of the surface ligands of the obtained copper sulfide nanoparticles needs to be considered

Method used

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  • Protein biological template-based gadolinium-doped copper sulfide nano-particles and preparation method thereof
  • Protein biological template-based gadolinium-doped copper sulfide nano-particles and preparation method thereof
  • Protein biological template-based gadolinium-doped copper sulfide nano-particles and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0032] (1) Accurately weigh 0.05mmol bovine serum albumin powder with an electronic balance and pour it into a 50mL single-mouth bottle, add 10mL phosphate buffer (0.01M), dissolve by ultrasonic vibration, and obtain a clear and transparent bovine serum albumin solution.

[0033] (2) Take 3mmol of CuCl respectively 2 2H 2 O and 5 mmol GdCl 3 ·6H 2 O was added to the solution in (1), and the stirring was continued for 5 min at room temperature.

[0034] (3) Prepare a sodium hydroxide solution with a concentration of 1M, add it to the reaction flask, and adjust the pH of the system to 8.

[0035] (4) Configure Na with a concentration of 4mmol / mL 2 S·9H 2 O solution, 0.4 mL was taken out and added to the above reaction system, and the reaction was continued for 12 h at 25 °C.

[0036] (5) After the reaction, the solution was taken out, added to a dialysis bag with a molecular weight cut-off of 8000-14000, placed in a beaker filled with secondary water, and dialyzed for 24 h...

Embodiment 2

[0039] (1) Accurately weigh 0.1 mmol of bovine serum albumin powder with an electronic balance and pour it into a 50 mL single-mouth bottle, add 10 mL of phosphate buffer (0.01 M), and dissolve by ultrasonic vibration to obtain a clear and transparent bovine serum albumin solution.

[0040] (2) Take 4.5mmol of CuCl respectively 2 2H 2O and 5 mmol GdCl 3 ·6H 2 O was added to the solution in (1), and the stirring was continued for 5 min at room temperature.

[0041] (3) Prepare a sodium hydroxide solution with a concentration of 1M, add it to the reaction flask, and adjust the pH of the system to 12.

[0042] (4) Configure Na with a concentration of 4mmol / mL 2 S·9H 2 O solution, 0.4 mL was taken out and added to the above reaction system, and the reaction was continued at 37° C. for 4 h.

[0043] (5) After the reaction, the solution was taken out, added to a dialysis bag with a molecular weight cut-off of 8000-14000, placed in a beaker filled with secondary water, and dial...

Embodiment 3

[0046] (1) Accurately weigh 0.15mmol bovine serum albumin powder with an electronic balance and pour it into a 50mL single-mouth bottle, add 10mL phosphate buffer (0.01M), dissolve by ultrasonic vibration, and obtain a clear and transparent bovine serum albumin solution.

[0047] (2) Take 6mmol of CuCl respectively 2 2H 2 O and 5 mmol GdCl 3 ·6H 2 O was added to the solution in (1), and the stirring was continued for 5 min at room temperature.

[0048] (3) Prepare a sodium hydroxide solution with a concentration of 1M, add it to the reaction flask, and adjust the pH of the system to 10.

[0049] (4) Configure Na with a concentration of 4mmol / mL 2 S·9H 2 O solution, 0.4 mL was taken out and added to the above reaction system, and the reaction was continued at 50° C. for 12 h.

[0050] (5) After the reaction, the solution was taken out, added to a dialysis bag with a molecular weight cut-off of 8000-14000, placed in a beaker filled with secondary water, and dialyzed for 48...

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Abstract

The invention discloses a gadolinium-doped copper sulfide nanoparticle based on a protein biological template and a preparation method thereof. The protein template is dissolved in PBS, configured into a solution with a concentration of 0.5-1.5mM, copper salt and gadolinium salt solution, and stirred Then add sodium hydroxide solution to adjust the pH to 8-12, then inject sulfur source, and react at 25°C-50°C; after dialysis and freeze-drying, GdCuS nanoparticle powder is obtained. The prepared GdCuS nanoparticles have a particle size of 6-10nm and are wrapped with protein on the outside. The photothermal conversion efficiency reaches 20%-30%, and the longitudinal relaxation rate r 1 =10mM·s ‑1 ‑18mM·s ‑1 . Higher heat will be generated under the irradiation of near-infrared light, and it is an ideal photothermal conversion agent and photoacoustic imaging contrast agent; the preparation steps of the invention are simple, and it is easy to prepare in large quantities; the reaction conditions are mild and energy consumption is reduced; the environmental compatibility is good , is a green synthetic process.

Description

technical field [0001] The invention relates to gadolinium-doped copper sulfide (GdCuS) nanoparticles and a preparation method thereof, belonging to the technical field of synthesis of inorganic nanomaterials, in particular to a bionic controlled synthesis of GdCuS nanoparticles based on bovine serum albumin as a biological template. Background technique [0002] Cancer has been the "public enemy number one" that has threatened human health and life for centuries. Currently, there are three traditional treatment methods for cancer: surgical resection, radiotherapy, and chemotherapy. However, each treatment method has drawbacks. For example, surgical resection can only eliminate part of the tumor cells and is prone to recurrence; radiotherapy and chemotherapy are likely to cause damage to normal tissues, with relatively high toxicity and side effects, and lack of specific treatment for tumor cells. In recent years, emerging phototherapy methods such as photothermal therapy ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/18A61K49/14A61K49/22A61K41/00A61P35/00
CPCA61K49/1869A61K41/0052A61K49/0002A61K49/143A61K49/225
Inventor 常津杨维涛宫晓群王生郭伟圣武玉东
Owner TIANJIN UNIV
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