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Fluorescence/magnetic resonance/electron computed tomography three-mode imaging nano contrast agent and preparation method thereo

An electronic computer and nano-contrast agent technology, applied in the field of nano-materials, can solve the problems of poor penetration of short-wavelength fluorescence, lack of common good state, and difficulty in penetrating deep tissues, achieving simple synthesis, excellent optical properties, and easy surface The effect of functionalization

Inactive Publication Date: 2020-02-07
SUN YAT SEN UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The problem with this patent application is that although it synthesizes double rare earth doped carbon dots through a simple method and can be used for fluorescence / magnetic resonance / CT imaging
[0006] The emission peak of fluorescence imaging is in the blue light region. Due to the poor penetration of short-wavelength fluorescence in the body, it is difficult to penetrate deep tissues and cannot meet the imaging requirements of deep tissues.
Functionality of the three imaging modalities did not share an optimal state

Method used

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  • Fluorescence/magnetic resonance/electron computed tomography three-mode imaging nano contrast agent and preparation method thereo

Examples

Experimental program
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Effect test

Embodiment 1

[0027] see figure 1 , the preparation process of I / Gd-doped carbon dots (I / Gd@CDs) is as follows:

[0028] Step 1: Weigh 2.0g urea, 1.1g anhydrous citric acid, 0.88g iohexol and 0.6g GdCl 3 ·6H 2 O was dissolved in 20mL of DMF (N,N-dimethylformamide), and ultrasonically dispersed for 15 minutes to obtain a white mixed emulsion;

[0029] Step 2: Add the white mixed emulsion obtained in Step 1 into a polytetrafluoroethylene reactor, put it in an oven, and heat it at 160°C for 6 hours to obtain a reaction solution;

[0030] Step 3: Take out the reaction solution obtained in step 2, add 20mL sodium hydroxide solution, the solubility of the sodium hydroxide solution is 50mg / ml, oscillate and mix evenly to obtain a dark red solution; centrifuge the dark red solution at 12000rpm for 30 minutes, The supernatant was taken, put into a dialysis bag (molecular cut-off flow: 100), dialyzed in pure water for 3 days, and the solution obtained after dialysis was freeze-dried to obtain the ...

Embodiment 2

[0033] The preparation process of I / Gd-doped carbon dots (I / Gd@CDs) is as follows:

[0034] Step 1: Weigh 2.0g urea, 1.0g anhydrous citric acid, 0.44g iohexol and 1.2g GdCl 3 ·6H 2 O was dissolved in 30mL of DMF (N,N-dimethylformamide), and ultrasonically dispersed for 20 minutes to obtain a white mixed emulsion;

[0035] Step 2: Add the above-mentioned white mixed emulsion into a polytetrafluoroethylene reactor, put it in an oven, and heat it at 180°C for 6 hours to obtain a reaction solution;

[0036] Step 3: Take out the above reaction solution, add 30mL sodium hydroxide solution, the solubility of sodium hydroxide solution is 50mg / ml, oscillate and mix evenly to obtain a dark red solution; centrifuge the dark red solution at 12000rpm for 30 minutes, and take the supernatant , added into a dialysis bag (molecular cut-off flow rate of 100), dialyzed in pure water for 5 days, and the solution obtained after dialysis was freeze-dried to obtain the product I / Gd@CDs, which was s...

Embodiment 3

[0039] The preparation process of I / Gd-doped carbon dots (I / Gd@CDs) is as follows:

[0040] Step 1: Weigh 2.0g urea, 1.2g anhydrous citric acid, 1.32g iohexol and 0.6g GdCl 3 ·6H 2 O was dissolved in 20mL of DMF (N,N-dimethylformamide), and ultrasonically dispersed for 30 minutes to obtain a white mixed emulsion;

[0041] Step 2: Add the above-mentioned white mixed emulsion into a polytetrafluoroethylene reactor, put it in an oven, and heat it at 140°C for 12 hours to obtain a reaction liquid;

[0042] Step 3: Take out the above reaction solution, add 40mL sodium hydroxide solution, the solubility of the sodium hydroxide solution is 50mg / ml, shake and mix evenly to obtain a dark red solution; centrifuge the above dark red solution at 12000rpm for 30 minutes, and take the supernatant Solution was added into a dialysis bag (molecular cut-off flow rate of 100), dialyzed in pure water for 4 days, and the solution obtained after dialysis was freeze-dried to obtain the product I / G...

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Abstract

The invention discloses a preparation method of a fluorescence / magnetic resonance / electron computed tomography three-mode imaging nano contrast agent, which comprises the following steps: step 1: carrying out ultrasonic treatment on anhydrous citric acid, urea, a compound containing I and a compound containing Gd in N, N-dimethylformamide to obtain a mixed emulsion; 2, adding the mixed emulsion into a polytetrafluoroethylene reaction kettle, and carrying out sealed high-temperature reaction; and 3, treating the product obtained in the step 2 with a sodium hydroxide solution, centrifuging, dialyzing a supernatant in water to obtain a dialysate, and freeze-drying the dialysate to obtain the I / Gd doped carbon dots. Meanwhile, the invention further discloses the nano contrast agent for fluorescence / magnetic resonance electron computed tomography three-mode imaging, and the contrast agent is simple and convenient to synthesize, environmentally friendly, easy for surface functionalization, relatively high in longitudinal relaxation rate and X-ray attenuation rate, and excellent in biocompatibility and blood compatibility.

Description

technical field [0001] The invention relates to the technical field of nanomaterials, in particular to a fluorescence / magnetic resonance / computer tomography three-mode imaging nano contrast agent and a preparation method thereof. Background technique [0002] Molecular imaging technology is playing an increasingly important role in the field of medical imaging. It can visualize cell functions and molecular reaction processes without destroying the living body, making it widely concerned in the fields of early diagnosis of cancer and basic research. At present, there are a variety of molecular imaging technologies clinically. Although each imaging method has the ability of independent imaging, due to the limitation of its imaging principle, each has its own defects, which makes accurate and rapid data collection only rely on a single imaging mode. Acquisition becomes difficult, so the combination of multiple imaging modes has become a research hotspot. Here, the imaging prob...

Claims

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Application Information

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IPC IPC(8): A61K49/00A61K49/06A61K49/04
CPCA61K49/0002A61K49/0067A61K49/04A61K49/06
Inventor 易长青赵俊凯
Owner SUN YAT SEN UNIV
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