Preparation method of gamma-PGA (polyglutamic acid) hydrogel loaded with Au nanoparticles

A nanoparticle and hydrogel technology, applied in the field of preparation of γ-PGA hydrogel, to achieve uniform particle size distribution, significant contrast effect, and remarkable contrast effect

Inactive Publication Date: 2016-06-15
DONGHUA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Retrieval of domestic and foreign literature found that there is still no information on the use of NaBH 4 Related reports on the preparation of γ-PGA hydrogels as CT imaging contrast agents using PEI-stabilized Au nanoparticles synthesized by reduction method as cross-linking agents

Method used

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  • Preparation method of gamma-PGA (polyglutamic acid) hydrogel loaded with Au nanoparticles
  • Preparation method of gamma-PGA (polyglutamic acid) hydrogel loaded with Au nanoparticles
  • Preparation method of gamma-PGA (polyglutamic acid) hydrogel loaded with Au nanoparticles

Examples

Experimental program
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Effect test

Embodiment 1

[0042] (1) Dissolve 240 mg mPEG-COOH (Mw=2kDa) in 10 mL DMSO, add 230 mg EDC (dissolved in 5 mL DMSO) and stir for 2 h to activate COOH; add 140 mg NHS (dissolved in 5 mL DMSO) and continue stirring for 1 h. After that, 100mgPEI (Mw=25kDa) was dissolved in 20mLDMSO and added to the above solution to continue stirring for 3 days. Finally, a dialysis bag with a molecular weight cut-off of 8000-14000 was dialyzed with distilled water for 3 days, and PEI NH was obtained after lyophilization. 2 -mPEG powder; (2) Take 100mgPEI NH prepared above 2 - Dissolve mPEG in 100mL ultrapure water, add 12.7mLHAuCl 4 solution (10mg / mL), stirred for 0.5h. Then add ice-bathed 58.2 mg NaBH to it 4 (dissolved in 5ml ultrapure water) solution, continue to stir for 2h, and finally use a dialysis bag with a molecular weight cut-off of 8000-14000 to dialyze with distilled water for 3 days, and obtain (Au 0 ) 200 -PEI·NH 2 -mPEG nanoparticle powder; (3) Dissolve 20mg γ-PGA in 2mL ultrapure water, a...

Embodiment 2

[0044] Get the γ-PGA / [(Au 0 ) 200 -PEI·NH 2 -mPEG] nano hydrogel, which was diluted to an appropriate concentration with ultrapure water, and used to measure surface potential and hydrodynamic diameter. Zeta potential measurement results show that γ-PGA / [(Au 0 ) 200 -PEI·NH 2 -mPEG] The surface potential of the nanohydrogel is -13.1±0.32mV, thus effectively avoiding the (Au 0 ) 200 -PEI·NH 2 -Cytotoxicity produced by amino groups on the surface of mPEG nanoparticles, also demonstrated by γ-PGA and (Au 0 ) 200 -PEI·NH 2 - Successful crosslinking of mPEG. γ-PGA / [(Au 0 ) 200 -PEI·NH 2 -mPEG] The hydrodynamic diameter of the nano hydrogel is 210.8 ± 6.35nm, the particle size distribution is uniform, and the hydrodynamic diameter can remain almost unchanged for a long time (see figure 1 ), thus illustrating that the γ-PGA / [(Au 0 ) 200 -PEI·NH 2 -mPEG] nanohydrogels have good colloidal stability. γ-PGA / [(Au 0 ) 200 -PEI·NH 2 -mPEG] nanohydrogel and (Au 0 ) 20...

Embodiment 3

[0046] Measure the γ-PGA / [(Au 0 ) 200 -PEI·NH 2 -mPEG] the content of Au element in the nano hydrogel. γ-PGA / [(Au 0 ) 200 -PEI·NH 2 -mPEG] nanometer hydrogel aqueous solution 2mL, measure the CT imaging picture of material under different Au concentration by CT imaging analyzer. By preparing a medical iohexol solution with the same I concentration as a comparison, it is proved that γ-PGA / [(Au 0 ) 200 -PEI·NH 2 - The superiority of mPEG] nanohydrogels for CT imaging.

[0047] γ-PGA / [(Au prepared by Example 1 0 ) 200 -PEI·NH 2 -mPEG] nanohydrogel CT imaging pictures (see Figure 7 a) It can be seen that the prepared γ-PGA / [(Au 0 ) 200 -PEI·NH 2 -mPEG] The CT images of the nanohydrogels gradually become brighter with the increase of Au concentration. CT imaging of samples with different concentrations shows that γ-PGA / [(Au 0 ) 200 -PEI·NH 2 -mPEG] nanohydrogels have good in vitro imaging effects. By comparing the γ-PGA / [(Au 0 ) 200 -PEI·NH 2 -mPEG]nanohydro...

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Abstract

The invention relates to a preparation method of γ-PGA hydrogel loaded with Au nanoparticles, comprising: adding PEI solution to activated mPEG-COOH solution to obtain PEI·NH 2 -mPEG, add chloroauric acid after being dissolved in ultrapure water, stir, add sodium borohydride aqueous solution, get (Au 0 ) 200 -PEI·NH 2 -mPEG; Add sodium bicarbonate to the activated γ-PGA solution, then add it to the DCM solution of AOT to get a W/O emulsion, add it to the PVA solution to get a W/O/W polymer emulsion; ( Au 0 ) 200 -PEI·NH 2 - Add the ultrapure aqueous solution of mPEG into the polymer emulsion drop by drop, and stir to obtain it. The invention has simple process and low cost; the prepared hydrocoagulation has good X-ray attenuation performance, remarkable imaging effect, good water solubility, colloidal stability, cell compatibility and biocompatibility.

Description

technical field [0001] The invention belongs to the field of hydrogel preparation, in particular to a preparation method in gamma-PGA hydrogel loaded with Au nanoparticles. Background technique [0002] Computed tomography (CT) imaging has become one of the most widely used diagnostic detection techniques in clinic due to its high spatial resolution, deep penetration ability, simple image post-processing technology and low cost. In order to obtain higher resolution CT images, contrast agents for CT imaging have emerged as the times require. At present, the CT imaging contrast agents commonly used in clinical practice are mainly iodine (I)-containing contrast agents, such as iohexol (iohexol), iopromide (iopromide), iopamidol (iopamidol) and the like. However, the imaging efficiency of this type of contrast agent is not very high, so in order to obtain a better imaging effect, a large dose of injection is often required, so the side effects on the kidneys are also more obvio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/04
CPCA61K49/04
Inventor 史向阳朱建志孙文杰周本青周一伟彭琛
Owner DONGHUA UNIV
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