Gold nanoparticle doped fluorocarbon liposome and preparation method and application thereof

A technology of gold nanoparticles and fluorocarbon lipids, which is applied in the chemical field, can solve the problems of cheap iodine and other problems, and achieve the effects of simple preparation method, synergistically enhanced imaging effect, and excellent contrast effect

Inactive Publication Date: 2014-09-03
SHANGHAI NAT ENG RES CENT FORNANOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

And both have their own advantages and disadvantages, gold has a stronger X-ray absorption coefficient than iodine, but iodine is cheaper than gold

Method used

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  • Gold nanoparticle doped fluorocarbon liposome and preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0021] Dissolve 10% soybean lecithin and 5% cholesterol in 2 mL of ether by mass ratio, and mix well by ultrasonic. Then add PFOB with a mass ratio of 10%, and after continuing to sonicate for 1 hour, slowly add the solution dropwise to 10 mL of buffer solution with pH=7.4, and stir slowly until the ether is completely volatilized, that is, fluorocarbon liposomes, which can be For CT contrast agent. Its particle size is 135.4 nanometers and its CT intensity is 21.04.

Embodiment 2

[0023] (1) Dissolve 0.02 mol of hexadecylamine in 100 mL of chloroform, add 0.01 mol of gold perchlorate, after ultrasonic dissolution, add 20 mL of 0.01 mol of sodium borohydride ethanol solution to the solution, continue ultrasonication for 2 hours, and end reaction. The reactant was rotary evaporated to 5 mL, 400 mL of absolute ethanol was added, and stood at -20°C for 20 hours to precipitate the product, which was washed with absolute ethanol and dried naturally.

[0024] (2) Dissolve 10% soybean lecithin, 5% cholesterol, and 1% gold nanoparticles in 2 mL of ether in a mass ratio, and mix well by ultrasonication. Then add PFOB with a mass ratio of 10%, and continue to sonicate for 1 hour, then slowly add the solution dropwise to 10mL buffer solution with pH=7.4, and stir slowly until the ether is completely volatilized, then you can get gold nanoparticles doped fluorocarbon Liposomes, which can be used as CT contrast agents. Its particle size is 158.2 nm, and its CT inte...

Embodiment 3

[0026] (1) Dissolve 0.02 mol of hexadecylamine in 100 mL of chloroform, add 0.01 mol of gold perchlorate, after ultrasonic dissolution, add 20 mL of 0.01 mol of sodium borohydride ethanol solution to the solution, continue ultrasonication for 2 hours, and end reaction. The reactant was rotary evaporated to 5 mL, 400 mL of absolute ethanol was added, and stood at -20°C for 20 hours to precipitate the product, which was washed with absolute ethanol and dried naturally.

[0027] (2) Dissolve 10% soybean lecithin, 5% cholesterol, and 10% gold nanoparticles in 2 mL of ether in a mass ratio, and mix well by ultrasonication. Then add PFOB with a mass ratio of 10%, and continue to sonicate for 1 hour, then slowly add the solution dropwise to 10mL buffer solution with pH=7.4, and stir slowly until the ether is completely volatilized, then you can get gold nanoparticles doped fluorocarbon Liposomes, which can be used as CT contrast agents. Its particle size is 201.1 nm, and its CT int...

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Abstract

The invention relates to a gold nanoparticle doped fluorocarbon liposome and a preparation method and application thereof. The method comprises the steps of: (1) preparation of hydrophobic gold nanoparticles; and (2) preparation of gold nanoparticle doped fluorocarbon liposome by using the hydrophobic gold nanoparticles obtained in the step (1) as the reactant. The preparation method provided by the invention is simple, has strong operability and can further satisfy the needs of production and application. The prepared CT contrast agent has good physical and chemical stability, can be used as a double-element CT imaging system, and has collaborative reinforcement imaging effect, and the contrast effect better than that of a pure fluorocarbon contrast agent.

Description

technical field [0001] The invention relates to the chemical field, and relates to a gold nanoparticle-doped fluorocarbon liposome and a preparation method thereof, which can be used to enhance the CT imaging effect of a fluorocarbon contrast agent. Background technique [0002] Liquid fluorocarbon is a kind of perfluorocarbon, which has high stability, good oxygen-carrying function, long circulation half-life, and was first used as a blood substitute. In terms of contrast imaging, the first high-pressure homogenization method was used to prepare lipid-encapsulated nano-scale liquid fluorocarbon emulsions for ultrasonic imaging, and achieved good results. Further related studies have reported that liquid fluorocarbon contrast agents can be used for targeted imaging and treatment as well as their use in CT and MRI, and it is proposed that liquid fluorocarbon nanoparticles also have the potential to become an ideal multimodal, multifunctional molecule. Potential of imaging co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/04A61K9/127
Inventor 何丹农朱君周涓程杰军
Owner SHANGHAI NAT ENG RES CENT FORNANOTECH
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