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Kit for rapidly detecting animal hydatid natural infection antibody

A technology for natural infection and detection of animals, applied in biological testing, measuring devices, material inspection products, etc., can solve the problem that antibody detection reagents cannot distinguish between vaccine immune antibodies and natural infection antibodies

Pending Publication Date: 2020-06-09
NANJING YIKE POPULATION HEALTH RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies in the prior art, the present invention provides a kit for rapidly detecting antibodies against natural echinococcosis in animals, which solves the existing methods for diagnosing echinococcosis in livestock. Genetic engineering of echinococcosis has been widely used in large quantities, and antibody detection reagents cannot The problem of distinguishing antibodies immunized by vaccines from antibodies produced by natural infection

Method used

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  • Kit for rapidly detecting animal hydatid natural infection antibody
  • Kit for rapidly detecting animal hydatid natural infection antibody

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Experimental program
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Effect test

Embodiment 1

[0025] The raw materials include by weight: Reagent A: Hyd dilution 20mmol / L, buffer 90mmol / L, DNA sequence solution 0.03mmol / L, chromogenic solution A0.2mmol / L; Reagent B: Hyd dilution 20mmol / L, Hyd enzyme conjugate 0.5mmol / L, buffer 90mmol / L, chromogenic solution B 0.1mmol / L.

[0026] In the present invention, the pH value of the buffer solution in reagent A is 7.0, and the pH value of the buffer solution in reagent B is 7.0.

[0027] In the present invention, the number of molecules of the DNA sequence liquid in reagent A is 3000.

[0028] In the present invention, the Hyd enzyme conjugate in the reagent B is the hydatid cyst fluid antigen purified by SPG.

[0029] In the present invention, the kit for rapidly detecting the natural infection antibody of animal echinococcosis, its detection method specifically comprises the following steps:

[0030] S1. Sample collection: Take animal serum or plasma samples, then divide the serum or plasma samples into two parts and put th...

Embodiment 2

[0034] The raw materials include by weight: Reagent A: Hyd dilution 25mmol / L, buffer 100mmol / L, DNA sequence solution 0.04mmol / L, chromogenic solution A0.3mmol / L; Reagent B: Hyd dilution 25mmol / L, Hyd enzyme conjugate 0.6mmol / L, buffer 100mmol / L, chromogenic solution B 0.2mmol / L.

[0035] In the present invention, the pH value of the buffer solution in reagent A is 7.2, and the pH value of the buffer solution in reagent B is 7.2.

[0036] In the present invention, the number of molecules of the DNA sequence liquid in reagent A is 4000.

[0037] In the present invention, the Hyd enzyme conjugate in the reagent B is the hydatid cyst fluid antigen purified by SPG.

[0038] In the present invention, the kit for rapidly detecting the natural infection antibody of animal echinococcosis, its detection method specifically comprises the following steps:

[0039] S1. Sample collection: Take animal serum or plasma samples, then divide the serum or plasma samples into two parts and put th...

Embodiment 3

[0043] The raw materials include by weight: Reagent A: Hyd dilution 30mmol / L, buffer 110mmol / L, DNA sequence solution 0.05mmol / L, chromogenic solution A0.4mmol / L; Reagent B: Hyd dilution 30mmol / L, Hyd enzyme conjugate 0.7mmol / L, buffer 110mmol / L, chromogenic solution B 0.3mmol / L.

[0044] In the present invention, the pH value of the buffer solution in reagent A is 7.4, and the pH value of the buffer solution in reagent B is 7.4.

[0045] In the present invention, the number of molecules of the DNA sequence liquid in reagent A is 5000.

[0046] In the present invention, the Hyd enzyme conjugate in the reagent B is the hydatid cyst fluid antigen purified by SPG.

[0047] In the present invention, the kit for rapidly detecting the natural infection antibody of animal echinococcosis, its detection method specifically comprises the following steps:

[0048] S1. Sample collection: Take animal serum or plasma samples, then divide the serum or plasma samples into two parts and put ...

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Abstract

The invention discloses a kit for rapidly detecting an animal hydatid natural infection antibody. The kit comprises the following raw materials in parts by weight: a reagent A including 20-30 mmol / L of a Hyd diluent, 90-110 mmol / L of a buffer solution, 0.03-0.05 mmol / L of a DNA sequence solution and 0.2-0.4 mmol / L of a developing solution A; a reagent B including 20 to 30mmol / L of Hyd diluent, 0.5to 0.7 mmol / L of Hyd enzyme conjugate, 90 to 110mmol / L of buffer solution and 0.1 to 0.3 mmol / L of developing solution B. The invention relates to the technical field of the detection of the naturalinfection antibody of the hydatid. The invention discloses the kit for rapidly detecting the animal hydatid natural infection antibody, the DNA sequence solution in the reagent A and a vaccine immuneantibody are used for recognition, the color development is carried out by using the color development solution A; then, the Hyd enzyme conjugate in the reagent B is used for being specifically combined with the hydatid natural infection antibody; the development solution B is matched for development such that the antibody generated by natural infection can be selectively detected, the interference of the vaccine immune antibody is avoided, the infection of hydatid disease can be quickly, simply, conveniently and accurately identified and diagnosed, and the problem that the antibody detectionreagent cannot distinguish the vaccine immune antibody from the antibody generated by natural infection is effectively avoided.

Description

technical field [0001] The invention relates to the technical field of detection of echinococcosis natural infection antibody, in particular to a kit for rapidly detecting animal echinococcosis natural infection antibody. Background technique [0002] Hydatid disease is a zoonotic parasitic disease caused by the larvae of Echinococcus tapeworm in the human body. It is endemic and is called endemic parasitic disease; it has the characteristics of occupational damage in endemic areas and is listed as an occupational disease of certain groups of people; globally speaking, it is a common disease unique to ethnic minorities or religious tribes and frequently-occurring diseases. Echinococcosis is a chronic parasitic disease caused by echinococcus larvae (echinococcus, echinococcosis) parasitizing human tissues. Echinococcosis is a disease caused by the larvae of Echinococcus species, Echinococcus granulosus, Echinococcus multilocularis, Echinococcus voluteri, and Echinococcus ol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6854G01N2333/43543
Inventor 江玥戴俊程何元林
Owner NANJING YIKE POPULATION HEALTH RES INST CO LTD
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