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Downy mildew bacterium culture and observation method

A technology for downy mildew and downy mildew, applied in the field of downy mildew cultivation and observation, can solve the problems of limited observation, slow research progress, inability to count zoospores released by sporangia of downy mildew

Pending Publication Date: 2020-02-25
NINGXIA UNIVERSITY
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Problems solved by technology

The zoospores released by the sporangia of downy mildew and the statistics of the number of zoospores cannot be counted. There are no detailed reports on the five key developmental stages before invading the host, such as swimming, forming stationary spores, and germination to form appresses.
Furthermore, the observation of the activity process before the downy mildew invades the host is limited, resulting in slow progress of related research

Method used

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  • Downy mildew bacterium culture and observation method
  • Downy mildew bacterium culture and observation method
  • Downy mildew bacterium culture and observation method

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Embodiment Construction

[0029] The present invention is further illustrated below, and it should be understood that the following specific embodiments are only used to illustrate the present invention and are not intended to limit the scope of the present invention. This patent provides multiple parallel schemes, and the different expressions belong to the improved scheme or the parallel scheme based on the basic scheme. Each scheme has its own unique characteristics.

[0030] The elements included in the technology: collect fresh downy mildew bacteria in the field (the ones collected in the morning can be used directly, and the ones collected in the afternoon need to be treated with moisturizing culture). Angular lesions) scabs, observe the off-white-grey black mold layer that grows on the back of the scabs, that is, the cyst peduncle and sporangia of downy mildew; pack into a clean plastic bag, and bring it back to the laboratory for subsequent use. Collect as much as possible in the morning befor...

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Abstract

The invention relates to the technical field of biology, in particular to a downy mildew bacterium culture and observation method. The method comprises the following steps: collecting downy mildew bacteria; preparing sporangium suspension liquid; and culturing the sporangium suspensions. Through repeated tests, a technology capable of inducing sporangium of cucumber downy mildew to germinate andrelease zoospore is obtained, the zoospore forms static spores through movement and morphological changes, the static spores germinate to form appressorium, and the appressorium germinates to generateinfection filaments. The technology can provide an effective technique for researchers to deeply research downy mildew biological basis, disease resistance identification, bactericide screening and the like. By means of the method, researchers can achieve quantitative statistics and monitoring of the number of zoospore of downy mildew, morphological changes and the number of formed infectors, andresearch of the action mechanism of the bactericide can also be achieved. Several development stages which must be experienced before downy mildew bacteria invade a host are clearly observed. The systematic observation method can provide first-hand data for prevention and treatment of the pathogenic bacteria on symptomatic drugs.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to methods for cultivating and observing downy mildew bacteria. Background technique [0002] Cucumber downy mildew is an important disease in cucumber production, and its spreading speed is very fast. Lesions formed on leaves often lead to dry leaf tissue, which has a serious impact on cucumber production. A lot of research has been carried out at home and abroad on the prevalence of cucumber downy mildew in the field, its correlation with environmental temperature and humidity, and the establishment of early warning models. However, the research on the biological basis of cucumber downy mildew (Pseudoperonospora cubensis) is relatively weak. The important reason is the lack of relevant observation and experiment techniques, which restricts the research on the biological basis of downy mildew. Cucumber downy mildew as an oomycete cannot be cultivated artificially, which limits the res...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12Q1/06G01N21/84C12R1/645
CPCC12N1/14C12Q1/06G01N21/84G01N2333/37G01N2021/8411
Inventor 史娟田芸瑞马新
Owner NINGXIA UNIVERSITY
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