Culture medium for prompting Phytophthora capsici Leonian to generate zoosporangium

Abstract

The invention particularly discloses a culture medium for prompting Phytophthora capsici Leonian to generate zoosporangium, belonging to the technical field of culture mediums. The culture medium is prepared from the steps of slicing 200g of peeled carrots into pieces, boiling in 1500ml of boiling water for 30 minutes, filtering, extracting filtrate, adding water into the filtrate until the solution is 1000ml, adding 15g of agar, uniformly stirring, carrying out sterilization at 121 DEG C for 15 minutes, so as to obtain the culture medium for prompting Phytophthora capsici Leonian to generate zoosporangium. The culture medium is prepared from yellow carrots and is capable of effectively inducing and prompting the Phytophthora capsici Leonian to generate zoosporangium, so that the problem that zoosporangium is very difficulty generated from Phytophthora capsici Leonian strains P2 and P3 on a red carrot culture medium is solved, and the inoculation requirement of a lots of zoospores can be effectively met, thereby being favorable to the quantitative research of researchers on pathogenicity of these strains.

Description

technical field [0001] The invention belongs to the technical field of culture medium, and relates to a culture medium for promoting zoosporangium production by Phytophthora capsici, in particular to a culture medium for promoting zoosporangia produced by Phytophthora capsici strains which are difficult to produce zoosporangia. Background technique [0002] Carrot medium is generally used in research to cultivate Phytophthora capsici. However, because Phytophthora capsici strains P2 and P3 were difficult to produce zoosporangia on red carrot medium, they could not meet the requirement of obtaining a large number of zoospores for inoculation. Therefore, it is necessary to explore a medium that can promote the formation of sporangia that is not easy to produce to induce the formation of zoosporangia, and then complete the inoculation experiment. Contents of the invention [0003] The purpose of the present invention is to overcome the shortcomings and deficiencies in the pr...

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Problems solved by technology

However, Phytophthora capsici strains P2 and P3 were difficult to produce zoosporangia on red carrot medium, so they could not meet the requirement of obtaining a large number of zoospores for inoculation

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