55results about How to "Induced generation" patented technology

The invention relates to a production method and application of a high-efficient cellulase mixture. Through performing fermentation by using a non-soluble carbon source, not only can fermentation cost of cellulase be saved, but also the production of the cellulase is effectively induced; a reducing sugar control measure is adopted, so that the repression effect of a carbohydrate is reduced, and the production capacity of the cellulase is improved; in addition, a novel material-making up control manner is built through combining the non-soluble carbon source with a material-making up component and a material-making up rate process, so that matching of all components of the produced cellulase mixture is optimized while the yield (production capacity) of the cellulase is promoted, and the activity of the cellulase is improved by not less than 50 percent.

The invention belongs to a filed of medicine technology, which relates to a novel pair of panaxsapogenol and a medical application of a mixture thereof. The pair of compounds are 20(R)-25-methoxyl-dammarane-3beta, 12beta, 20-triol(25Rmdt for short) and 20(S)-25-me-thoxyl-dammarane-3beta, 12beta, 20-triol(25Smdt for short). The 25Rmdt and 25Smdt and the mixture thereof have the antitumor activities of inhibiting the growth and multiplication of a plurality of tumor cells, inducing the differentiation and withering of tumor cells, inhibiting the generation of tumor angiogenic blood vessels, inhibiting the invasion and transfer of tumors, enhancing the body immunity and reducing the toxic and side effects of chemotherapeutic drugs, which is substantially used for treating malignant tumors, with a total effective dosage of 1-100mg / kg / d.

The invention provides medicated tea for conditioning and treating diabetes, and a preparation method thereof. The medicated tea comprises the following drug substances in parts by weight according to the formula: 1-5 parts of fructus momordicae, 7-12 parts of herba gynostemmatis pentaphylli, 3-12 parts of hawthorn fruit, 3-8 parts of radix ophiopogonis, 2-6 parts of lotus leaves, 1-6 parts of ganoderma lucidum, 3-8 parts of fragrant solomonseal rhizome, 2-6 parts of fructus momordicae flower, 2-6 parts of barbary wolfberry fruit, 1-4 parts of liquorice, 1-5 parts of capillary wormwood herb and 2-6 parts of membranous milkvetch roots. The medicated tea can maintain weight aiming at the situation that weight gradually declines, improves metabolic parameters, reduces hyperglycemia on an empty stomach and after dinner, prevents or reduces complications of diabetes, and reduces hypertriglyceridemia on an empty stomach and after dinner.

The invention particularly discloses a culture medium for prompting Phytophthora capsici Leonian to generate zoosporangium, belonging to the technical field of culture mediums. The culture medium is prepared from the steps of slicing 200g of peeled carrots into pieces, boiling in 1500ml of boiling water for 30 minutes, filtering, extracting filtrate, adding water into the filtrate until the solution is 1000ml, adding 15g of agar, uniformly stirring, carrying out sterilization at 121 DEG C for 15 minutes, so as to obtain the culture medium for prompting Phytophthora capsici Leonian to generate zoosporangium. The culture medium is prepared from yellow carrots and is capable of effectively inducing and prompting the Phytophthora capsici Leonian to generate zoosporangium, so that the problem that zoosporangium is very difficulty generated from Phytophthora capsici Leonian strains P2 and P3 on a red carrot culture medium is solved, and the inoculation requirement of a lots of zoospores can be effectively met, thereby being favorable to the quantitative research of researchers on pathogenicity of these strains.

The invention provides a Yersinia pestis ghost, a preparation method thereof, and application of the Yersinia pestis ghost serving as a vaccine. The ghost has a complete outer membrane structure, can induce an organism to generate a protective antibody by inoculating a mouse without an adjuvant, generate strong immune memory and protect the mouse from being attacked by strong dose of plague live strains, and has higher safety compared with a live vaccine.

The invention belongs to the field of genetic engineering techniques disclosing the recombinant and application of intelligent adenovirus vector and khp53 anti-oncogene. The invention specifically involves recombinant viral vector of human tumor-suppressing gene, especially involves human p53 tumor-suppressing gene recombination intellectual adenovirus vector conducted by Kozak sequence, and also involves drug combinations with the gene recombinant vector. Through the combination utilization of elements enhancing eukaryotic gene expression such as MCMV promoter, intron and Kozak regular sequence etc, the problem of relative shortage expression of the p53 gene is solved; and through adopting AdMAX HiIQ recombinant adenovirus vector system, the problem of effective packaging exogenous gene high expressing the recombined adenovirus effectively, and finally,through site-specific homologous recombination in eukaryotic cells, 5-recombinated adenovirus rAdMH-khp53 with E1, E3 deleted is packaged out. Utilizing recombinant adenovirus of the invention, clinical-used gene drugs can be prepared for prevention and treatment of a variety of cancer.

Disclosed are a combined vaccine for hepatitis A and hepatitis E, and preparation method thereof. Said vaccine comprises hepatitis E virus recombined peoteins, hepatitis A attenuated live vaccine and hepatitis A inactivated vaccine. Said method comprises before the paretion of the final combined vaccine, absorbing hepatitis A attenuated live vaccine viruses and / or hepatitis A inactivated vaccine viruses on aluminum hydroxide gel, absorbing hepatitis E virus recombined peoteins on aluminum hydroxide gel, adjusting the pH value of the two compositons, and mixing the two compositons together. The inventiion is provided with convenience, multiple-effect, low cost, and effective prevention of hepatitis A and hepatitis E for human and animals.

The invention provides a feed capable of preventing and treating a porcine reproductive and respiratory syndrome. The feed comprises the following raw materials: an immune preparation for preventing and treating the porcine reproductive and respiratory syndrome, corn, soybean meal, fish meal, calcium hydrophosphate, wheat, edible salt, composite additives of trace elements, a multivitamin additive, lysine and methionine. The included immune preparation is capable of enhancing the cellular immunity and the humoral immunity of the organism through the organism regulation action, activating immune cells, inducing generation of interferon and preventing survival and reproduction of viruses in porcine somatic cells, and plays a role in resisting the porcine reproductive and respiratory syndrome.

The invention relates to the technical field of livestock breeding, in particular to a culture method for improving immunity of pig bodies and preventing and controlling pig diseases. The culture method comprises the following steps: utilizing mixtures of traditional Chinese medicine compositions and base feeds to feed pigs, wherein the traditional Chinese medicine compositions are composed of astragalus roots, white atractylodes rhizomes, epimedium herbs, pogostemon cablin, isatis roots, isatis roots, honeysuckle flowers, baical skullcap roots, drying rehmannia roots, divaricate saposhniovia roots and liquorice; and utilizing inactivated hog cholera vaccines or newcastle vaccines as interferon Inducers for inoculation of pigs. The traditional Chinese medicine compositions help significantly improve immunity of pigs due to the combination of interferon for induction and control. The culture method for improving immunity of pig bodies and preventing and controlling pig diseases has the following beneficial effects: the overall health states of pig groups can be improved; pigs can enjoy good appetite, smooth skin, normally exhaust manures and urine without tear spots and few bleeding spots in the skin; swelling of lymph glands is relieved; cough, diarrhea and slow growth and decrease in premonition are basically controlled; and occurrences of diseases are few; and pigs are healthy and free from epidemic diseases.

The invention discloses a method for inducing nematode-trapping fungi to generate a trapping organ by using nematode extracellular vesicles. The method comprises the following steps of (1) culturing nematodes; (2) obtaining the nematode extracellular vesicles and carrying out characterization analysis on the nematode extracellular vesicles; (3) culturing the Arthrobotrys oligospora which is the nematode trapping fungus; and (4) inducing generation of Arthrobotrys oligospora trapping organs by using the nematode extracellular vesicles. According to the method, Extracellular vesicles (EVs) of caenorhabditis elegans are extracted, and the obtained EVs are utilized to successfully induce Arthrobotrys oligospora to generate a trapping organ, so that the EVs secreted by the nematode contain a substance for inducing nematode-trapping fungi to generate the trapping organ; and the method has important significance for understanding an interaction mechanism of nematodes and nematode-trapping fungi, and has important application value in the field of nematode biological control.

The invention discloses exfoliating gel. According to a formula, the exfoliating gel comprises the following components in part by weight: 50 to 60 parts of lavender hydrosol, 0.5 to 0.7 part of carboxymethyl cellulose sodium, 2 to 4 parts of hyaluronic acid, 1 to 2 parts of coenzyme Q10, 2 to 4 parts of single fruit acid extract, 1 to 3 parts of grapefruit extract and 0.6 to 1.0 part of preservative. The exfoliating gel has natural ingredients, is safe and non-irritating when used, can remove aged and necrotic cutin, can also induce the generation of new skin and brighten skin color, and ensures that the skin is relatively soft, smooth and tender.

The invention specifically relates to a nutrient microcapsule for rehabilitation of tapping panel dryness, and a preparation method and application thereof, belonging to the technical field of rubberagriculture. The nutrient microcapsule for rehabilitation of tapping panel dryness in the invention adopts a conventional nutrient for rehabilitation of tapping panel dryness as a core material and amononuclear structure formed by cross-linking of chitosan / sodium alginate / glutaraldehyde as capsule wall material. The formed nutrient microcapsule has sustained release effect, can improve the symptoms of tapping for latex discharge, and significantly increases the yield of latex.

The invention provides a preparation method of a culture medium for rapid propagation of jasminum sambac, and belongs to the field of jasminum sambac propagation. The method comprises the following steps: 1. preparation of a primary culture medium: KT, CTK, saccharose, asparagus cochinchinensis extract and agar are sequentially added into a MS culture medium according to the formula until to be completely dissolved, pH value is adjusted, and then sterilization under high temperature is carried out; 2. preparation of a proliferation medium: the KT, ethylic acid, the saccharose and the agar aresequentially added into the MS culture medium according to the formula until to be completely dissolved, the pH value is adjusted, the sterilization under high temperature is carried out, and heteroauxin is added after cooling; 3. preparation of a rooting medium: the KT, the saccharose, coconut juice, tender willow juice and the agar are sequentially added into the MS culture medium according to the formula, the pH value is adjusted, and then the sterilization under high temperature is carried out. The invention has the beneficial effects that the preparation method is simple and easy to operate, low in cost and friendly to environment; different culture mediums are definitely provided for the rapid propagation of the jasminum sambac at different stages; different demands of the jasminum sambac in different energy substances and effective substances are met at the different propagation stages; seedlings are promoted to healthily grow; the seeding rate is increased; the seedlings are strong.

The invention discloses a plant antifreezer and its preparation method. The plant antifreezer is preparegggggd from the following components of: by weight, 0.5-0.97% of glucose, 0.5-0.97% of cane sugar, 0.2-0.5% of potassium dihydrogen phosphate, 0.18-0.21% of novel monomolecular lipid film, 0.06-0.17% of paclobutrazol, 0.2-0.33% of amino acid, 0.02-0.05% of 6-benzylamino-purine, 0.01-0.05% of rapin, 46-48.4% of glycerin and 48.6-51% of water. The plant antifreezer provided by the invention is nontoxic and harmless, doesn't pollute the environment, and can be directly sprinkled on plant leaf surfaces, flowers and flower bus. The preparation method is simple and requires low cost. Effective antifreezing period can reach 15-20 days. By the adoption of the plant antifreezer, lower temperature freeze injury resistance of plants can be enhanced and the freeze injury can be rapidly mitigated after freeze injury. The plant antifreezer provided by the invention can be used to form a protection film on the surface of a plant and protect the plant from cold, and has an effect of preventing diseases.

The invention relates to a biological strain. Rice straw and rice husk are used as main raw materials, corn powder and chitin are used as auxiliary materials to prepare a culture medium; soybean milkand brown sugar are used as a nutritional solution, and EM bacteria is used as a bacterial source to prepare a bacterial solution; and the culture medium is fermented into the biological strain by using the bacterial solution. In the implementation process of the invention, the rice straw and the rice husk are used as the main raw materials, the corn powder and the chitin are used as the auxiliarymaterials, and the soybean milk and the brown sugar are used for preparing the nutritional solution, so that the prepared culture medium is rich in nutrition; the culture medium preparing from the rice straw and the rice husk is loose in structure, so that when the EM strain is proliferated, the EM strain can be multiplied at multiple levels in a short time, the fermentation effect is 3 to 5 times of that of the traditional method, and the strain is vigorous in growth and high in vitality. By adding the chitin in the implementation process, the formed biological strain not only has the efficacy of the biological strain, but also has the advantages and functions of the chitin.

The invention discloses a cyclometalated iridium metformin complex and a preparation method and application thereof. Firstly the cyclometalated iridium metformin complex is provided, and the complex not only can be rapidly absorbed by tumor cells, but also has a phosphorescence tracing characteristic; the complex has excellent hypoxic anti-tumor activity, compared with the complex under a normal oxygen condition, the cytotoxicity of the complex to tumor cells under the hypoxic condition is enhanced, ROS can be better induced to be generated in the tumor cells, the complex has good inhibitory activity to HIF-1 alpha, and finally apoptosis of the tumor cells is induced. The invention further provides a preparation method of the complex. The preparation method is simple and low in cost. Therefore, the complex has a good application prospect in preparation of hypoxic antitumor drugs.

The invention relates to a cell-assembly based pathological model for metabolism syndrome and the application thereof to high content drug screening. By assembling the extracellular matrix material of fat stem cells and stimulation cells, a cell-containing 3D structure with a certain porosity can be formed. By manipulating the inducing differentiation conditions, the fat stem cells on the surface of the internal channel of the 3D structure can be differentiated into endothelial cells, and the fat stem cell inside the matrix material differentiate into fat cells. The pancreatic islets separated from animals are directly arranged into the pores of the 3D structure to from a plurality of secretion regulation points. By providing the system models long time stimulus from energy metabolism regulation matters, such as glucose with high concentration, fatty acids insulin, TNF or fat cells secreting factors, etc; the fat cells, islet cells and endothelial cells can be induced to show pathological symptoms of metabolic syndrome. The invention also provides a method for applying the above mentioned pathological model of metabolic syndrome to drug screening.

The invention relates to a montmorillonite substrate layer material capable of improving the fracture resistance of cement mortar. The montmorillonite substrate layer material is obtained by mixing and wet-grinding montmorillonite, an intercalation modifier and water. According to the invention, a small amount of the montmorillonite substrate layer material capable of improving the fracture resistance of the cement mortar, provided by the invention, is doped into a cement-based material, so that the early-stage compressive strength and fracture resistance of cement can be remarkably improved; and when 1-3% of the montmorillonite substrate layer material is doped into the cement-based material, the 1d compressive strength of the cement mortar can be improved by 6-45%, and the 1d fracture resistance can be improved by 25-53%.

The invention relates to a porcine circovirus type 3 virus-like particle as well as a construction method and application thereof, and belongs to the technical field of preparation of virus-like particles. The invention provides a recombinant plasmid, the recombinant plasmid takes a pSMA plasmid as a basic carrier, an optimized Cap full-length gene is inserted, and the nucleotide sequence of the optimized Cap full-length gene is as shown in SEQ ID NO. 1. The defects of an existing eukaryotic expression system and a traditional prokaryotic expression system are overcome, the preparation method for soluble expression of complete Cap protein of the porcine circovirus type 3 and successful realization of the virus-like particles by using the prokaryotic expression system becomes possible, and the obtained virus-like particles are high in immunogenicity and low in cost.

The invention relates to the technical field of livestock breeding, and in particular to a traditional Chinese medicinal composition for improving pig immunity and for preventing and treating porcine viral diseases as well as a product and a feeding method of the traditional Chinese medicinal composition. The traditional Chinese medicinal composition consists of radix astragali, largehead atractylodes rhizome, dioscorea opposita from Henan of China, herba epimedii, herba pogostemonis, radix isatidis, honeysuckle flowers, radix scutellariae, radix rehmanniae from Henan of China, radix saposhnikoviae and radix glycyrrhizae. With the application of the traditional Chinese medicinal composition provided by the invention, pig immunity can be obviously enhanced, so that the morbidity of the viral diseases can be reduced, and a survival rate and a growth performance can be improved.

The invention relates to the technical field of livestock breeding, and in particular to a traditional Chinese medicinal composition for improving pig immunity and for preventing and treating porcine viral diseases as well as a product and a feeding method of the traditional Chinese medicinal composition. The traditional Chinese medicinal composition consists of radix astragali, largehead atractylodes rhizome, dioscorea opposita from Henan of China, herba epimedii, herba pogostemonis, radix isatidis, honeysuckle flowers, radix scutellariae, radix rehmanniae from Henan of China, radix saposhnikoviae and radix glycyrrhizae. With the application of the traditional Chinese medicinal composition provided by the invention, pig immunity can be obviously enhanced, so that the morbidity of the viral diseases can be reduced, and a survival rate and a growth performance can be improved.

The invention relates to the technical of grapes, in particular to an inducing hormone for seedless Kyoho grapes, which comprises a pre-blossoming hormone and a post-blossoming hormone, wherein the pre-blossoming hormone comprises, by weight, 40-60 parts of GA3, 80-120 parts of SM, 40-60 parts of 6-BA, 10-20 parts of amino acids, and 0.4-1 part of a spreader, and the post-blossoming hormone comprises, by weight, 40-60 parts of GA3, 4-6 parts of CPPU, 10-20 parts of amino acids, and 0.4-1 part of a spreader. The inducing hormone can effectively induce seedless grapes, with seedless rate reaching 97% and above; maturation day of grapes can be effectively brought forward, and fruit stem hardening chances are slimmed.

The invention relates to the technical field of livestock breeding, in particular to a culture method for improving immunity of pig bodies and preventing and controlling pig diseases. The culture method comprises the following steps: utilizing mixtures of traditional Chinese medicine compositions and base feeds to feed pigs, wherein the traditional Chinese medicine compositions are composed of astragalus roots, white atractylodes rhizomes, epimedium herbs, pogostemon cablin, isatis roots, isatis roots, honeysuckle flowers, baical skullcap roots, drying rehmannia roots, divaricate saposhniovia roots and liquorice; and utilizing inactivated hog cholera vaccines or newcastle vaccines as interferon Inducers for inoculation of pigs. The traditional Chinese medicine compositions help significantly improve immunity of pigs due to the combination of interferon for induction and control. The culture method for improving immunity of pig bodies and preventing and controlling pig diseases has the following beneficial effects: the overall health states of pig groups can be improved; pigs can enjoy good appetite, smooth skin, normally exhaust manures and urine without tear spots and few bleeding spots in the skin; swelling of lymph glands is relieved; cough, diarrhea and slow growth and decrease in premonition are basically controlled; and occurrences of diseases are few; and pigs are healthy and free from epidemic diseases.

The invention discloses food with a function of preventing hyperlipidemia of the middle-aged and aged people. The food is made from components in parts by weight as follows: 20-35 parts of corns, 10-30 parts of lucid ganoderma, 10-30 parts of buckwheat, 20-40 parts of gingkoes, 15-30 parts of pollen pini and 15-30 parts of salviae miltiorrhizae.

The invention discloses a drug for controlling bursa of Fabricius in poultry. Composition of the drug is as follows: 10 parts of toad meat by weight, 10 parts of pallas pit viper by weight, 8 parts ofwindpipe trachea by weight, 8 parts of honeysuckle flower by weight and 6 parts of astragalus root by weight. The formula of the drug has the advantages of relatively and thoroughly combining the effect of antivirus, anti-bacteria and immunity improvement, and very good curative effect.

The invention discloses a cyclometalated iridium metformin complex and a preparation method and application thereof. Firstly the cyclometalated iridium metformin complex is provided, and the complex not only can be rapidly absorbed by tumor cells, but also has a phosphorescence tracing characteristic; the complex has excellent hypoxic anti-tumor activity, compared with the complex under a normal oxygen condition, the cytotoxicity of the complex to tumor cells under the hypoxic condition is enhanced, ROS can be better induced to be generated in the tumor cells, the complex has good inhibitory activity to HIF-1 alpha, and finally apoptosis of the tumor cells is induced. The invention further provides a preparation method of the complex. The preparation method is simple and low in cost. Therefore, the complex has a good application prospect in preparation of hypoxic antitumor drugs.

The invention discloses a pterostilbene-modified cyclometal iridium (III) complex and a preparation method and application thereof. The cyclometal iridium (III) complex modified based on the natural product pterostilbene has good fat solubility, tumor cell uptake ability and anti-tumor cell proliferation ability, can effectively inhibit tumor cell proliferation, induce ROS generation, reduce mitochondrial membrane potential and induce tumor cell autophagy, thereby having good anti-tumor activity; therefore, the cyclometal iridium (III) complex of the invention has a better effect than cisplatin drugs under the premise of small toxic and side effects; based on the ability of the complex of the invention to inhibit tumor cell proliferation, damage tumor cell mitochondrial function and induce tumor cell autophagy, it has broad application prospects in the preparation of anti-tumor drugs and anti-tumor drug components.

The invention provides a dental implant provided with multiple sections of threads. The dental implant comprises a dental crown and an implant, wherein the implant consists of an implant head, an implant neck and an implant root which are arranged sequentially from top to bottom; a groove is formed in the dental crown, the implant head is embedded into the groove, and the implant head is in interference fit with the groove of the dental crown; the implant root is provided with three sections of threads which are respectively a first thread section arranged at an upper part of the implant root,a second thread section arranged at a middle part of the implant root, and a third thread section arranged at a lower part of the implant root; and a tooth form depth of the first thread section is less than that of the second thread section. The dental implant provided by the invention has improved initial stability, is firmly fixed, effectively avoids the looseness of the dental implant, and reduces the stress in an alveolar bone, thereby improving the long-term stability of the implant, inducing osteoclastogenesis, promoting bone interface fusion, reducing bone resorption around the implant root, prevents bacteria from entering and being capable of widely applied to various implant denture restoration processes for hypodontia.

The invention belongs to a filed of medicine technology, which relates to a novel pair of panaxsapogenol and a medical application of a mixture thereof. The pair of compounds are 20(R)-25-methoxyl-dammarane-3beta, 12beta, 20-triol(25Rmdt for short) and 20(S)-25-me-thoxyl-dammarane-3beta, 12beta, 20-triol(25Smdt for short). The 25Rmdt and 25Smdt and the mixture thereof have the antitumor activities of inhibiting the growth and multiplication of a plurality of tumor cells, inducing the differentiation and withering of tumor cells, inhibiting the generation of tumor angiogenic blood vessels, inhibiting the invasion and transfer of tumors, enhancing the body immunity and reducing the toxic and side effects of chemotherapeutic drugs, which is substantially used for treating malignant tumors, with a total effective dosage of 1-100mg / kg / d.