Preparation method of culture medium for rapid propagation of jasminum sambac

A proliferation medium and culture medium technology, applied in the field of jasmine reproduction, can solve the problems of single jasmine cultivars, low natural seed setting rate of jasmine, and reduced flower yield, shorten the reproduction cycle, improve seedling breeding and reproduction efficiency, and the preparation method is simple and easy line effect

Inactive Publication Date: 2019-02-15
饶一凡
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the jasmine cultivar is single. For a long time, only the local bilobal jasmine in Hengxian County, Guangxi has been cultivated, and the pests and diseases have gradually increased. The offspring are seriously

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A preparation method for a jasmine rapid propagation medium, comprising the steps of:

[0036] Preparation of primary medium: Add 0.12mg / L KT, 5mg / L CTK, 22g / L sucrose, 12g / L asparagus extract, 8g / L agar to MS medium in sequence until completely dissolved, adjust the pH to 6.4, and Autoclave at 121°C for 15 minutes; CTK in the primary medium can promote cell elongation and division, and prepare for further proliferation and culture. Asparagus extract can effectively prevent browning and corruption of jasmine tissues, and promote the smooth division of its tissue cells , differentiation, improve the vigor and robustness of the obtained tissue culture seedlings, and facilitate subsequent proliferation and rooting culture;

[0037] Prepare the proliferation medium: add 0.25mg / L KT, 1.5g / L acetic acid, 25g / L sucrose, and 3g / L agar to the MS medium in sequence until completely dissolved, adjust the pH to 6.4, and sterilize under high pressure at 121°C for 15 minutes. After ...

Embodiment 2

[0043] A preparation method for a jasmine rapid propagation medium, comprising the steps of:

[0044] Preparation of primary medium: Add 0.15mg / L KT, 10mg / L CTK, 25g / L sucrose, 15g / L Asparagus extract, 10g / L agar to MS medium in sequence until completely dissolved, adjust the pH to 6.6, and Autoclave at 121°C for 25 minutes; CTK in the primary medium can promote cell elongation and division, and prepare for further proliferation and culture. Asparagus extract can effectively prevent browning and corruption of jasmine tissues, and promote the smooth division of its tissue cells , differentiation, improve the vigor and robustness of the obtained tissue culture seedlings, and facilitate subsequent proliferation and rooting culture;

[0045] Preparation of proliferation medium: Add 0.3mg / L KT, 1.2mg / L(-)-8-phenylmenthol, 2g / L acetic acid, 28g / L sucrose, 5g / L agar to MS medium sequentially until completely dissolved , adjust the pH to 6.6, autoclave at 121°C for 25min, after cooli...

Embodiment 3

[0051] A preparation method for a jasmine rapid propagation medium, comprising the steps of:

[0052] Preparation of primary medium: Add 0.14mg / L KT, 8mg / L CTK, 24g / L sucrose, and 8g / L agar to MS medium sequentially until completely dissolved, adjust pH to 6.5, and autoclave at 121°C for 20 minutes; CTK in the medium can promote cell elongation and division, and prepare for further proliferation and culture. Asparagus extract can effectively prevent browning and corruption of jasmine tissues, promote the smooth division and differentiation of tissue cells, and increase the obtained tissue The vitality and robustness of the seedlings are conducive to subsequent proliferation and rooting cultivation;

[0053]Preparation of proliferation medium: Add 0.28mg / L KT, 1mg / L(-)-8-phenylmenthol, 1.8g / L acetic acid, 26g / L sucrose, 4g / L agar to MS medium sequentially until completely dissolved , adjust the pH to 6.5, autoclave at 121°C for 20min, after cooling, add 5.5mg / L indole acetic a...

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Abstract

The invention provides a preparation method of a culture medium for rapid propagation of jasminum sambac, and belongs to the field of jasminum sambac propagation. The method comprises the following steps: 1. preparation of a primary culture medium: KT, CTK, saccharose, asparagus cochinchinensis extract and agar are sequentially added into a MS culture medium according to the formula until to be completely dissolved, pH value is adjusted, and then sterilization under high temperature is carried out; 2. preparation of a proliferation medium: the KT, ethylic acid, the saccharose and the agar aresequentially added into the MS culture medium according to the formula until to be completely dissolved, the pH value is adjusted, the sterilization under high temperature is carried out, and heteroauxin is added after cooling; 3. preparation of a rooting medium: the KT, the saccharose, coconut juice, tender willow juice and the agar are sequentially added into the MS culture medium according to the formula, the pH value is adjusted, and then the sterilization under high temperature is carried out. The invention has the beneficial effects that the preparation method is simple and easy to operate, low in cost and friendly to environment; different culture mediums are definitely provided for the rapid propagation of the jasminum sambac at different stages; different demands of the jasminum sambac in different energy substances and effective substances are met at the different propagation stages; seedlings are promoted to healthily grow; the seeding rate is increased; the seedlings are strong.

Description

technical field [0001] The invention belongs to the field of jasmine propagation, in particular to a preparation method for a medium for rapid propagation of jasmine. Background technique [0002] Jasmine [Jasminum sambac (L.) Aiton] is an evergreen shrub of the genus Jasminum in the family Oleaceae, with an upright or half-open crown, native to tropical and subtropical regions. There are more than 60 common jasmine varieties, such as creeping jasmine, woody jasmine, pearl jasmine, and foreign jasmine, which can be divided into three types: single-lobe jasmine, double-lobe jasmine, and multi-lobe jasmine according to their flower shape. China is the country with the largest jasmine production in the world, and its annual output accounts for more than 60% of the world's total production. Now it is widely planted in Guangxi, Fujian, Guangdong, Yunnan and other places. Among them, Guangxi is the largest production area of ​​jasmine in my country, accounting for 80% of the natio...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G24/20A01G24/22A01G24/30
CPCA01H4/001A01G24/20A01G24/22A01G24/30
Inventor 饶一凡
Owner 饶一凡
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