Fermentation process for culturing schizochytrium limacinum with high protein content

Abstract

The invention belongs to the field of fermentation of schizochytrium limacinum, and particularly relates to a method for producing high-protein-content schizochytrium limacinum through fermentation by using an allogenic material as a substrate. The method mainly comprises the following steps: 1) placing soybean molasses in a container, and heating in a water bath kettle at 80-100 DEG C for 0.5-1.5 h; (2) after the water bath is completed, putting the soybean molasses into a centrifugal machine, carrying out centrifugal precipitation for 5-20 minutes at 4000-8000 rpm, and collecting molasses supernate; (3) adding the molasses supernate into a container, respectively adding peptone, yeast extract, sea crystal and a defoaming agent into the container, dissolving and sterilizing for later use; and (4) inoculating schizochytrium limacinum into the prepared culture medium according to the inoculum size of 4-6%, and carrying out fermentation culture for 72-120 hours. The fermentation method is simple in process, low in pollution, high in protein content, low in cost and suitable for large-scale industrial production.

Description

technical field [0001] The invention belongs to the field of fermentation of Schizochytrium sp., and in particular relates to a method for fermenting Schizochytrium sp. with high protein content by using exogenous substances as substrates. Background technique [0002] As a traditional large agricultural country, our country has a large number of livestock and poultry, so a large amount of feed is needed to provide nutrition for animals. As an important part of the animal body, protein needs to be ingested from the outside world. Studies have shown that Schizochytrium is composed of several substances such as protein and lipid, among which the content of protein accounts for about 15%, and by optimizing the fermentation method, Schizochytrium with higher protein content can be obtained. [0003] At present, there are few types of conventional protein feeds on the market in our country, and the cost is relatively high, so a low-priced protein feed substitute is needed. Trad...

AI Technical Summary

Problems solved by technology

Traditional fermentation methods usually use glucose as a carbon source, but the price of glucose is high, and the nutrient substance of glucose is single, resulting in high fermentation cost and low protein content

Molasses replaces some glucose as a carbon source, which can reduce the cost of fermentation, and molasses is rich in various trace elements, which can supplement the consumption of trace elements during the fermentation process. At present, there is still a lack of molasses combined with trace elements to optimize the fermentation parameters of Schizochytrium sp. reported that further research in this area is urgently needed