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Hepatitis A-hepatitis E combined vaccine and preparation method thereof

A technology for hepatitis A and hepatitis E, which is applied in the field of hepatitis A-hepatitis E combined vaccine and its preparation, can solve problems that have not been reported yet, achieve reduced production costs and market prices, good safety and immunogenicity sex, increase the effect of immunogenicity

Inactive Publication Date: 2006-12-27
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the joint vaccine of hepatitis A and hepatitis E, which is intestinally transmitted hepatitis, has not been reported so far at home and abroad.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A kind of HAV-HEV combination vaccine, comprises hepatitis E virus recombinant protein and hepatitis A attenuated live vaccine or hepatitis A inactivated vaccine, said hepatitis A attenuated live vaccine, its virus titer is 5-10LogCCID 50 / ml, or hepatitis A inactivated vaccine, its virus antigen content is 250-1000U / ml, and the content of hepatitis E virus recombinant protein is 5-50ug / ml.

[0024] This embodiment also includes an immune adjuvant, the immune adjuvant is an aluminum hydroxide gel solution, and the concentration range of the aluminum hydroxide gel is adjusted to 0.6-1.5mg / ml,

[0025] The above-mentioned hepatitis A live attenuated vaccine is hepatitis A attenuated live vaccine L-A-1, H2 vaccine strain or other available vaccine strains; hepatitis A inactivated vaccine is hepatitis A virus HM175 strain or other available vaccine strains; The amino terminal of the hepatitis virus recombinant protein is located between the 380 and 480 amino acids encoded b...

Embodiment 2

[0029] A method for preparing the above-mentioned HAV-HEV combination vaccine:

[0030](a) Before preparing the final HAV-HEV combined vaccine, the hepatitis A attenuated live vaccine virus and / or the hepatitis A inactivated vaccine virus are adsorbed on aluminum hydroxide gel, and the hepatitis E virus recombinant protein is adsorbed on Aluminum hydroxide gel, adjust the pH to 5.5-9.6;

[0031] (b) The above-mentioned components whose pH was adjusted are mixed.

[0032] Further examples are given below.

Embodiment 3

[0033] Embodiment 3: Preparation of hepatitis A attenuated live vaccine virus stock solution

[0034] Human diploid fibroblast MRC5 was cultured with minimal medium (MEM) (pH 7.2-7.6) containing 10% calf serum. After the cells formed a dense monolayer, the growth medium was discarded, and the cells were washed repeatedly with Hanks' solution After that, inoculate the pre-prepared hepatitis A virus seed solution. After adsorption at 34-36°C for 2 hours, add cell growth maintenance solution to the culture, and culture at 34-36°C for 4 weeks, changing the medium 1-2 times a week. After culturing, the maintenance solution and residual calf serum were discarded, and the 199 comprehensive culture solution containing 3mM NaCl but not containing phenol red was directly added to continue culturing at 34-36°C for 3-4 days. Then the cells are collected, centrifuged after repeated freezing and thawing and cell disruption, and the collected supernatant is the stock solution of the live at...

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Abstract

Disclosed are a combined vaccine for hepatitis A and hepatitis E, and preparation method thereof. Said vaccine comprises hepatitis E virus recombined peoteins, hepatitis A attenuated live vaccine and hepatitis A inactivated vaccine. Said method comprises before the paretion of the final combined vaccine, absorbing hepatitis A attenuated live vaccine viruses and / or hepatitis A inactivated vaccine viruses on aluminum hydroxide gel, absorbing hepatitis E virus recombined peoteins on aluminum hydroxide gel, adjusting the pH value of the two compositons, and mixing the two compositons together. The inventiion is provided with convenience, multiple-effect, low cost, and effective prevention of hepatitis A and hepatitis E for human and animals.

Description

technical field [0001] The invention relates to a combination vaccine and a preparation method thereof, in particular to a hepatitis A-hepatitis E combination vaccine and a preparation method thereof. Background technique [0002] In recent years, with the rapid development of my country's economy, the flow of people has increased, and the occurrence of various infectious diseases has become increasingly frequent, which seriously threatens the health of the public and causes a huge economic burden to the country. Therefore, in order to effectively control the prevalence of infectious diseases, research and development of preventive vaccines have important theoretical and practical significance. [0003] Hepatitis A (Hepatitis A) and Hepatitis E (Hepatitis E) are acute viral hepatitis transmitted through the fecal-oral route. The common forms of hepatitis A and Hepatitis E in my country are epidemic and sporadic. In 1988, the acute hepatitis A epide...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/29A61P31/14A61P1/16
CPCY02A50/30
Inventor 孟继鸿戴星董晨
Owner SOUTHEAST UNIV
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