4 results about "Hepatitis a virus" patented technology

The invention discloses a recombinant duck enteritis virus for expressing 3-type duck hepatitis A virus immunogenic genes and a construction method and application thereof. The recombinant duck enteritis virus is characterized in that an eukaryotic expression cassette containing a type 3 duck hepatitis A virus immunogenic gene is inserted into a non-coding region between UL26 and USL27 genes of a duck enteritis virus genome. According to the invention, a duck hepatitis A virus (DHAV) immunogenic gene is inserted into a duck enteritis virus (DEV) genome at a fixed point by virtue of a CRISPR / Cas9 gene editing technology, so that the recombinant duck enteritis virus for expressing the duck hepatitis A virus type 3 immunogenic gene is constructed and obtained. Moreover, based on the recombinant duck enteritis virus for expressing the 3-type duck hepatitis A virus immunogenicity gene constructed by the invention, the exogenous DHAV immunogenicity gene can be stably expressed in the recombinant virus, and the recombinant virus has good in-vitro replication ability and hereditary stability and can be used for preparing animal vaccines.

The present invention relates to a hepatitis A virus production method and hepatitis A virus produced according to the method, and, more specifically, to: a hepatitis A virus production method and hepatitis A virus produced according to the method, the method comprising the step of infecting a host cell with a virus obtained by transforming a host cell with a vector comprising an expression cassette, which comprises a hepatitis A virus gene, for hepatitis A virus preparation, and subculturing same.

The application belongs to the field of immunoassay and biosensing technology, and provides a preparation method of an ECL sensor based on near-infrared alkyne functionalized Cu NSs and a double-cycle waste-free Cas12a driven signal amplifier. Specifically, alkyne functionalized Cu NSs are used as luminophores, and the unique alkyne structure and rigid conformation of the ligand BPTA not only realize pi-pi ordered stacking, but also inhibit non-radiative transition and promote the formation of highly conjugated aggregates, so that the Cu NSs exhibit stable and efficient NIR-ECL performance at 803 nm wavelength, excellent biocompatibility and low cost advantage. In addition, based on the DNA recycling and reuse realized by the designed double-cycle strategy, and the excellent reaction kinetics and specificity of the Cas12a system, the double-cycle waste-free Cas12a driven signal amplifier not only solves the problem of DNA waste liquid in the traditional cycle, but also overcomes the defects of low cutting efficiency and poor specificity existing in the traditional enzyme cutting method. According to the difference in electrochemiluminescence signal intensity of different concentrations of the measured substance, the detection of hepatitis A virus HAV is realized.

This invention discloses the application of duck IL7 protein as an adjuvant in the preparation of duck hepatitis A virus type 3 inactivated vaccine, belonging to the field of biopharmaceuticals. The research of this invention shows that duck IL7 protein as an adjuvant can enhance the immunoprotective efficacy of the DHAV-3 inactivated vaccine and also enhance its heterologous protective ability. This provides a theoretical basis for the development of duck IL7 as a novel duck vaccine adjuvant.