Pharmaceutical use of ent-eudesmane alcohol type sesquiterpene for inhibiting hepatitis virus

A technology for enantiopaline and hepatitis B virus, applied to medical preparations containing active ingredients, antiviral agents, pharmaceutical formulas, etc., can solve the problems of not yet available and reduce hepatitis B surface antigen

Inactive Publication Date: 2007-03-28
赵昱
View PDF0 Cites 99 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present inventor made further research on the basis of the prior art, and mainly screened the anti-HBV activity of the phytochemical monomer components of this genus, and found that the enantio-eucalyptanes extracted from the medicinal plants of the genus Hexaria Sesquiterpene alcohols and glycosides have significant anti-hepatitis B virus while reducing the effect of hepatitis B surface antigen, and through literature review, so far, there is no treatment of this type of enantio-eucalyptane-type sesquiterpene alcohols and glycosides for hepatitis B virus infection Diseases and reports on preparation of anti-hepatitis B virus drugs

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Pharmaceutical use of ent-eudesmane alcohol type sesquiterpene for inhibiting hepatitis virus
  • Pharmaceutical use of ent-eudesmane alcohol type sesquiterpene for inhibiting hepatitis virus
  • Pharmaceutical use of ent-eudesmane alcohol type sesquiterpene for inhibiting hepatitis virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Inhibitory effect of formula (1) compound on the hepatitis B surface antigen (HBsAg) secreted by HepG2.2.15 cells

[0043] 1) Cell culture:

[0044] HepG2.2.15 cells were cultured in DMEM medium containing 10% inactivated fetal bovine serum, 100 U / ml penicillin and 100 μg / ml streptomycin, 100 μg / ml G418 at 37°C, 5% CO 2 , cultured in an incubator with 100% relative humidity.

[0045] 2) The inhibitory effect of the compound of formula (1) on HepG2.2.15 cell growth was measured by MTT method:

[0046] Take the HepG2.2.15 cells in the logarithmic growth phase and dilute the cells with medium to 1×10 5 / ml, seeded in 96-well cell culture plate, 100 μl per well, at 37°C, 5% CO 2 After cultivating in an incubator with 100% relative humidity for 24 hours, add the compound of formula (1) diluted with medium, the concentration is respectively 1000 μg / ml, 200 μg / ml, 40 μg / ml and 8 μg / ml, 200 μl in each well, each Concentration Set up three replicate wells, placed ...

Embodiment 2

[0054] Example 2 : formula (1) compound is to HepG2.2.15 cell secreted hepatitis B virus deoxyribonucleic acid (HBV-DNA) replication inhibitory effect

[0055] 1) Cell culture:

[0056] Method is with embodiment 1.

[0057] 2) MTT method was used to measure the inhibitory effect of the enantiocane-type sesquiterpene alcohol and glycoside compounds represented by formula (1) on the growth of HepG2.2.15 cells: the method was the same as in Example 1.

[0058] 3) Determination of the inhibitory effect of the enantio-eucalyptane-type sesquiterpene alcohol and glycoside compounds represented by formula (1) on the replication of hepatitis B virus deoxyribonucleic acid (HBV-DNA). Take the HepG2.2.15 cells in the logarithmic growth phase, and dilute the cells to 1×10 with medium 5 / ml, seeded in 96-well cell culture plate, 100 μl per well, at 37°C, 5% CO 2 After cultivating in the incubator of 100% relative humidity for 24 hours, add the enantio-eucalyptane-type sesquiterpene alc...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
melting pointaaaaaaaaaa
melting pointaaaaaaaaaa
Login to view more

Abstract

The invention relates to an enantiomorphic amine alkyl sesquiterpene alcohol and glucoside and the medicated salt or solvent thereof, as well as the effect and activity of the composed medicine combination, mainly relating to the medical use in reducing HBV-DNA replication activity. And it has considerably strong inhibiting effect on HBsAG screted by HepG2.2.15 and HBV-DNA replication as compared with positive contrast Lamivudine; and it has obvious inhibition activity to HBV-DNA replication at large dosage (100 mug / mL) and medium dosage(20 mug / mL) as contrasted with Lamivudine, and can be expected to apply to preparing medicines for curing HB virus infection disease.

Description

technical field [0001] The present invention relates to a class of enantio-eucalyptane-type sesquiterpene alcohols and glycosides, pharmaceutically acceptable salts or solvates thereof, and the pharmacodynamic activity of pharmaceutical compositions formed therefrom, mainly relating to its ability to reduce hepatitis B surface antigen and inhibit hepatitis B surface antigen. Medicinal use of hepatitis virus deoxyribonucleic acid (HBV-DNA) replication activity. technical background [0002] Hepatitis B (referred to as hepatitis B) is an infectious disease caused by hepatitis B virus (HBV), which is transmitted through blood and body fluids and has a chronic carrier state. The disease is widely prevalent in my country, and the infection rate among the population is high, and the infection rate reaches more than 35% in some areas. According to relevant data, the number of patients who tested positive for hepatitis has reached 189 million, while the number of people (carriers) ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C07C35/23C07H15/203C07H15/18A61K31/047A61K31/704A61K36/28A61P1/16A61P31/12
Inventor 赵昱刘光明于荣敏李海波巫秀美孙汉董孙先凤张荣平钱金袱郝小江白骅约阿施·史托克希特张礼和
Owner 赵昱
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap