The invention provides a rolling process capable of eliminating the 20MnCr5 steel as-rolled mixed crystal structure. The rolling process comprises the following steps: 1, selecting a billet of 20MnCr5 steel; 2, heating the steel billet; 3, performing rough rolling on the steel billet; 4, performing intermediate rolling on the steel billet; 5, performing finish rolling on the steel billet; and 6, cooling the steel to obtain a 20MnCr5 steel finished product finally. According to the method, after the temperature at which the 20MnCr5 steel starts dynamic recrystallization is accurately checked, the appropriate rolling starting temperature is determined, and rolling is started in the temperature interval higher than 1050 DEG C, so that distortion energy in the steel is accumulated, the recrystallization tendency is increased, and finally the effects of refining grains and eliminating mixed crystals are achieved. The uniformity, the grain size, the hardness and the mechanical property of the obtained 20MnCr5 steel as-rolled structure are greatly improved, and the production efficiency cannot be reduced due to process changes.