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204results about How to "Steady injection" patented technology

Recording Ink, Ink/Media Set, Ink Cartridge, Inkjet Recording Method and Inkjet Recording Apparatus

To provide a recording ink which contains at least a water-dispersible colorant, a water-dispersible resin, a wetting agent, a surfactant and water, wherein the content of the wetting agent in the recording ink is in the range of from 20% by mass to 35% by mass, the total solid content of the water-dispersible colorant and the water-dispersible resin in the recording ink is in the range of from 12% by mass to 40% by mass, the water-dispersible resin contains fluorine resin particulates, and the ratio A:B, where A is the solid content of the water-dispersible resin in the recording ink and B is the solid content of a pigment contained in the water-dispersible colorant in the recording ink, is in the range of 0.5 to 8.
Owner:RICOH KK

Mine gas beam tube monitoring system and recognition method for gas explosivity and fire danger

The invention relates to a mine gas beam tube monitoring system, comprising an automatic sampling device which consists of a gas path switching electromagnetic valve and a suction pump. The gas path switching electromagnetic valve is connected with a partial channel through a sampler and a single-core beam tube of a moisture separator and then a conduit head through a partial beam tube cable and is controlled by a programmable controller after a main beam tube cable is connected with a moisture separator box. The system is characterized in that the monitoring system also comprises an automatic sampling device which is composed of a sample making device. The sample making device consists of a non-oil injection pump and a conversion electromagnetic valve controlled by the programmable controller, a shunting needle-shaped valve with an emptying end, a dryer, a filtering device, a quantitative tube and a single-core beam tube of a flow meter which are successively connected through an automatically sampling pipe. The automatic sampling device is connected with a gas chromatography detection device for analyzing and outputting data to a computer. The computer controls the programmable controller and outputs detection results. The dryer and the filtering device are provided with long service life. The measuring point number extension is convenient and can be maintained easily. The cost is low. The sampling device samples steadily. The precision of detected gas is high. A recognition method for the gas explosiveness and the fire hazard is disclosed. The decision according to the analysis result is clear and prompt.
Owner:CCTEG SHENYANG RES INST

Thymalfasin-containing medicinal composition and preparation method thereof

The invention belongs to the field of medicaments, and particularly relates to a medicinal composition, in particular to a thymalfasin-containing medicinal composition and a preparation method thereof. The medicinal composition comprises the following components: thymalfasin, mannitol and sorbitol, wherein the weight ratio of the thymalfasin to the mannitol to the sorbitol is 16:(25-75):(25-75); and the thymalfasin, the mannitol and the sorbitol are processed and prepared into injection or freeze-dried powder injection. The medicinal composition has high medicinal stability.
Owner:HAINAN ZHONGHE PHARM CO LTD

Multi-functional soft contact lens mould

The invention relates to a moulding method of a soft contact lens. The preparation method of the mould comprises the following steps: 1) designing and processing a metal core, 2) performing injection molding of the mould; 3) curing and forming a lens; 4) performing hydration of the lens; and 5) packaging the lens. The lens mould of the invention can be used to satisfy the forming of the soft contact lens and used as the hydration container of a semi-finished lens and as the packaging container of a finished lens too. The invention also has the advantages of stable injection, simple hydration operation and low injection low.
Owner:JIANGSU HORIEN CONTACT LENS

Chill-free processing method of wind-driven generator hub

The invention discloses a chill-free processing method of a wind-driven generator hub, and the method comprises the following steps: manufacture of a mould, manufacture of a sand mould and moulding by casting, wherein in the manufacture of the sand mould, a completely opened bottom gating system is utilized; an ingate is designed to be disc-shaped; a downgate on an upper sprue is a quantitative ladle stopper sprue gate; the ratio of the components is F(straight):F(transverse):F(internal)=1:(1.5-2):(25-28); a rectangular vent hole is arranged at the highest point at the top face of a casting; the total section area of the rectangular vent hole is greater than that of the ingate; a casting head is arranged on the top face of the casting; the height of the single casting head is greater than 400 mm; no chills are discharged during the manufacture procedure; in the moulding by casting, the casting speed of melted iron entering into a casting cavity is controlled within 0.5-1.0 m / s; the casting time is controlled within 150-210 seconds; and the casting temperature is between 1300 and 1310 DEG C. The chill-free processing method of the wind-driven generator hub disclosed by the invention has the advantages that the method adapts to the large scale production of low-temperature ductile iron castings for large wind-driven generator with quite high requirements, the process is simple, and the production cost is low.
Owner:宁波日星铸业有限公司

Electromagnetic fuel injection valve

The invention provides an electromagnetic fuel injection valve which is operated extremely normally, is excellent in a durability, can always obtain a stable injection amount, and can reduce an influence by a fuel, an atmospheric temperature and the like. In an electromagnetic fuel injection valve (1) in which an electromagnetic valve (2) corresponding to a drive source is constituted by a yoke (21), a core (22) and a movable valve (23), an injection nozzle (41) interiorly provided with a ball valve (43) and having an injection port (42) for injecting a fuel is arranged in a leading end of the movable valve (23) so as to form an injection valve (4), and a return spring (3) bonded to the movable valve (23) and an adjusting screw (5) pressure supporting the return spring (3) are arranged in an axial hole (25) of the core (22) by being fitted and inserted to the axial hole (25) of the core (22) from an opening in a base end side, a connection lever (6) is slidably interposed between the adjusting screw (5) in the axial hole (25) of the core (22) and the return spring (3).
Owner:NIKKI CO LTD

Droplet injection device and nozzle recovery method for droplet injection device

The present invention aims to efficiently eliminate settling of solid particles in the ink, and enable long-term stable droplet injection. Specifically, a droplet injection device comprises an ink chamber, a nozzle corresponding to the ink chamber, an energy imparting unit, and a drive pulse generation unit. The energy imparting unit is impacted and driven by a drive pulse to impart energy to the ink in the ink chamber. The nozzle allows droplets to be sent out from the nozzle, so that a printing area for recording material is printed. The drive pulse generation unit generates a shoot-out pulse allowing a droplet to be sent out from the nozzle, and provides a micro vibration pulse as a drive pulse for the ink in the ink chamber based on a degree that the droplet is not sent out from the nozzle. The ink comprises dispersant, and solid particles with a proportion greater than the dispersant. An updating unit is also provided. The updating unit applies micro vibration motion of a plurality of micro vibration pulses and then a plurality of shoot-out pulses, so as to enable split motion with the same.
Owner:KONICA MINOLTA INC

Novel boiler main steam temperature regulating device and control method

InactiveCN107166360AAvoid Thermal Fatigue DamageGuaranteed sealingBoiler controlSuperheaterSteam temperature
The present invention is a novel boiler main steam temperature regulating device and control method, which includes: the steam pipeline before the primary superheater, the different-diameter tee in front of the primary superheater, the desuperheating steam pipeline, and the desuperheating steam pipeline regulating valve , desuperheating steam pipeline isolation valve, primary superheater, panel superheater, steam temperature measuring device for desuperheating steam pipeline, steam flow measuring device for desuperheating steam pipeline, steam pipeline before the inlet pipe group of secondary superheater , Different diameter tee in front of the inlet pipe group of the secondary superheater, inlet pipe group of the second superheater, outlet pipe group of the secondary superheater, main steam temperature measuring device, main steam pipeline, steam before the inlet pipe group of the secondary superheater Temperature measuring device, cascaded PID controller placed in decentralized control system. First of all, it avoids the situation that the normal injection of desuperheating water cannot be guaranteed due to the large pressure change of the desuperheating water source in the original desuperheating method; secondly, the temperature of the main steam will not be lowered too low, which protects the pipe wall; in addition, it avoids the main steam carrying water, Shock turbine.
Owner:NORTH CHINA ELECTRIC POWER UNIV (BAODING)

Cabazitaxel fat emulsion, and preparation method and use thereof

Provided in the present invention is a cabazitaxel fat emulsion injection, wherein the cabazitaxel fat emulsion injection contains cabazitaxel, a medium chain triglyceride for injection, and lecithin. Also provided in the present invention are the method for preparing the cabazitaxel fat emulsion injection and the use thereof in preparing a drug for treating prostate cancer.
Owner:JIANGSU TASLY DIYI PHARMA CO LTD

Monogastric animal bionic digestion system and method for simulating monogastric animal digestion based on the system

The invention discloses a single stomach animal biomimetic digestion system and the method for simulating the single stomach animal digestion. The system comprises: at least one simulated digestion device disposed on the fixed pawl of the air bath shaking table, at least one digestive juice bottle connected with the liquid transport tube; a main peristaltic pump connected with the input pipe; a waste liquid accepting bottle connected with the main peristaltic pump, at least one buffer liquid bottle connected with the main peristaltic pump and the output pipe; a cleaning fluid bottle connectedwith the main peristaltic pump, the cleaning fluid bottle is also connected with the output pipe, the cleaning fluid storage bottle and the lower residual cleaning fluid accepting bottle; the outputpipe is connected with or isolated from air through a connection control electromagnetic valve. The method comprises preparation and biomimetic digestion steps; the biomimetic digestion step comprises: injecting digestive juice, cycling buffer liquid, removing buffer liquid and cleaning residual liquid. The system and method in the invention realizes the the external simulation of the forage digestion and absorption process in animal gstrointestinal tract, achieves high fidelity and good repeatability.
Owner:INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI

Wooden door for entering room

ActiveCN107100523AControl inflationStop the air conditionerBuilding braking devicesSealing arrangementsEngineeringPiston
The invention relates to a wooden door, and specifically is a wooden door for entering a room. A wooden door body is connected on a wall body horizontally and rotatably. The wooden door is characterized in that the wooded door further comprises a plurality of air inflating mechanisms, an air inflating pipeline, a control mechanism and a expanding mechanism which are all mounted on the wooden door body; each air inflating mechanism comprises an air inflating component, a reset spring and a piston block, an air inflating cavity is formed in each air inflating component, and each air inflating cavity is provided with an air inflating port; and the piston blocks and the air inflating cavities are connected in a sliding and sealing mode, one ends of the reset springs are fixed to the ends, far away from the air inflating ports, of the air inflating cavities internally, and the other ends of the reset springs are fixedly connected to weight-balancing parts of the piston blocks. To solve the problem that a large gap must be arranged when an existing wooden door is mounted, so that a plenty of cool air and warm air overflows when the door is closed, the wooden door for entering the room aims to provide the wooden door which can still prevent the cool air and the warm air in the room from overflowing when the gap between the wooden door body and the ground is large.
Owner:CHONGQING YIJU DOOR

Experimental operation device for improving immunity of mouse

The invention discloses an experimental operation device for improving the immunity of a mouse. The experimental operation device comprises a base, wherein a first hydraulic cylinder is welded at thetop of the base, an output shaft of the first hydraulic cylinder is rotatably sleeved with a disc, a first locking mechanism is arranged on the disc, a second hydraulic cylinder is welded on one sideof the disc, an injection bucket is fixedly installed on an output shaft of the second hydraulic cylinder and is of a hollow structure, a push rod is arranged above the injection bucket, one end of the push rod extends into the injection bucket, a piston is fixedly installed at the end, located in the injection bucket, of the push rod, the piston is in tight contact with the inner wall of the injection bucket, a needle tube is arranged below the injection bucket, one end of the needle tube extends into the injection bucket, and a buffer device is arranged in the injection bucket. The experimental operation device is simple in structure and convenient to operate, can fix the mouse on the base, mechanically controls the push rod for injection, makes the injection more stable and prevents muscle hypertonicity from causing medicine rejection.
Owner:AFFILIATED HOSPITAL OF WEIFANG MEDICAL UNIV

Automatic contrast injection device

The invention relates to an automatic contrast injection device. According to the automatic contrast injection device, a medical tee joint is connected with a first injector, a second injector and aninfusion needle tubing and adjusts the route manners of the first injector, the second injector and the infusion needle tubing; a control device controls a first linear guide rail slider and a secondlinear guide rail slider to drive the first injector and a second injector to mutually push in air and normal saline, thereby forming and injecting size-uniform contrast bubbles stably into human bodyto achieve automation; further, a tee joint driving mechanism drives the valve body of the medical tee joint to rotate to further enhance automation; further, the automatic cooking robot is applicable to injectors of various specifications to meet the requirements on one load for multiple injection; further, the automatic cooking robot also comprises a blocking detection module, which can achieveself-locking during obstruction or displacement of the infusion needle tubing. On balance, the automatic cooking robot has the advantage of combining contrast agent and air into size-uniform and stable contrast agent bubbles and injecting the bubbles into human body, thereby achieving ultrasound contrast standardization and operation stability.
Owner:GUANGDONG GENERAL HOSPITAL

Solvent-assisted SAGD three-dimensional physical simulation experiment method and device

The invention provides a solvent-assisted SAGD three-dimensional physical simulation experiment method and device. The device comprises an injection device, a simulated oil reservoir model and a recycling device, a first pump unit in the injection device is communicated with a piston container unit, a second pump unit is communicated with a steam generator, the piston container unit and the steam generator are communicated with a gas injection well of the simulated oil reservoir model respectively, one end of a first condenser of the recycling device is communicated with a production well of the simulated oil reservoir model while the other end of the same is communicated with a feeding port of a gas-liquid separator, a second condenser is communicated with a liquid outlet of the gas-liquid separator, a third condenser is communicated with a gas outlet of the gas-liquid separator, a raw oil and water collection container is connected with the second condenser, and a solvent recycling container is connected with the third condenser. By the method and the device, mining conditions of solvent-assisted SAGD technology can be simulated, and mechanism of solvent in the process of SAGD can be studied so as to optimize and improve existing solvent-assisted SAGD technology according to oil reservoir conditions.
Owner:PETROCHINA CO LTD

Stable favipiravir injection and preparation method thereof

The invention relates to a stable favipiravir injection and a preparation method thereof. The stable favipiravir injection comprises favipiravir, a solubilizer sulfobutyl ether-beta-cyclodextrin sodium and an acid-base regulator. The stable favipiravir injection can endure terminal sterilization and has good safety and stability.
Owner:FUKANGREN BIO PHARMA

Cable-controlled intelligent stratification water injection device system and construction technique thereof

PendingCN109386266ARealize manual free fishingRealize the test and adjustment workSurveyCoupling contact membersEngineeringPressure data
The invention discloses a cable-controlled intelligent stratification water injection device system and a construction technique thereof. The system comprises a technique tubular column, a ground control device and a computer. The technique tubular column is connected with the computer through a steel pipe cable and the ground control device and provided with a cable-controlled intelligent water distribution device integrated with a vortex flowmeter, flow information of a measured medium is obtained through the vortex flowmeter, and the steel pipe cable is adopted for transmitting the downholestratification flow and stratification pressure data to the computer through the ground control device during normal water injection; the distribution and injection amount of a current layer is set through the computer, and real-time adjustment is conducted by comparing the transmission flow with the distribution and injection amount. The provided cable-controlled intelligent stratification waterinjection technique tubular column can not only meet the intelligent deployment of distribution and injection wells, reduce the phenomenon of nonuniform water absorption between layers and greatly improve the recovery efficiency but also automatically measure and adjust the downhole sub-section flow, store production data in real time and conduct stratum pressure reduction tests.
Owner:PETROCHINA CO LTD

Plug assembly for beverage container

Provided is a plug assembly for a beverage container, exhibiting a favorable pourability without hindering a compactness. The assembly includes: an outer plug (12) fitted into an upper opening of a container body (1) and having an outflow passage (16R) therein; and an inner plug (13) fitted into an upper opening section of the outer plug (12). Provided on the inner circumferential surface of the outer plug (12) is a female screw (21) having a thread continuous in the radial direction and used to attach the inner plug (13). Provided on the outer circumferential surface of the inner plug (13) is a male screw (22) corresponding to the female screw (21). Flow passages (61) are formed by vertically cutting out at least a part of the male screw (22), and provided on the upper regions of the flow passages (61) are control walls (64J) linearly slanting upward and outward. A stable screwed state can be consistently maintained regardless of the locations of the flow passages (61).
Owner:THERMOS LLC +1

Colloidal gold fluorescent quantitative analysis all-in-one machine and control method thereof

The invention discloses a colloidal gold fluorescent quantitative analysis all-in-one machine and a control method thereof. According to the all-in-one machine, through the application of a microprocessor, as well as an illumination unit, a shooting unit, an image processing unit, a laser device and a first photoelectric sensor which are connected with the microprocessor, the all-in-one machine disclosed by the invention can be used as a colloidal gold card reader and also can be used as an immunity fluoroanalyzer, so that the cost is reduced. The invention also provides a control method of the all-in-one machine, and whether a test paper card is a colloidal gold test paper card or an immunity fluorescence test paper card does not need to be distinguished by an operator in the using process, so that the experimental procedures are reduced, and the experimental efficiency is improved.
Owner:WUHAN J H BIO TECH

Soup syringe

Provided is a drug solution injection tool that is applicable for many hours without using any large-sized battery to continue the stable injection of the drug solution at a predetermined fixed injection speed, irrespective of the kinds and temperatures of the drug solution to be injected. The drug solution injection tool comprises a pressured drug solution dispensing means, a secondary pressure means in which the drug solution communicates with the pressured drug solution dispensing means via an upstream side channel, an upstream side open / close means located at the upstream side channel and opening or closing drug solution communication between the pressured drug solution dispensing means and the secondary pressure means, a downstream side open / close means located at a downstream side channel provided at the downstream side of the secondary pressure means, and a control means for controlling the open / close timing of the upstream and downstream side open / close means.
Owner:NIPRO CORP

Portable anesthetic clinical injection equipment

InactiveCN110860011AThe injection process is stableEasy to adjust preciselyAutomatic syringesMedical devicesElectric machineryAnesthesia
The invention discloses portable anesthetic clinical injection equipment, and relates to the technical field of anesthetic injection. Aiming at the problem of rapidly and stably injecting anesthetic into a patient, the invention provides the following scheme: the equipment comprises a frame body, a double-shaft motor is fixedly connected to the inner wall of the bottom of the frame body, first screw rods are installed at the output ends of the two sides of the double-shaft motor, a first screw rod is in threaded sleeve connection with a movable ring, a first supporting rod is hinged to the topof the outer ring of the movable ring, a baffle is hinged to the top end of the first supporting rod, a driving belt wheel is installed at the right end of each first screw rod on the right side, a driven belt wheel is arranged above the driving belt wheel, and a second screw rod is connected to the left side of the driven belt wheel through a bolt. The equipment not only can accurately adjust the position of anesthetic injection for a patient, replaces manual injection of medical staff, reduces injection failures, relieves pain of the patient, and shortens precious time for the medical staffto treat the patient.
Owner:THE AFFILIATED HOSPITAL OF QINGDAO UNIV

Vertical injection molding mechanism achieving efficient mold compounding and working method of vertical injection molding mechanism

The invention discloses a vertical injection molding mechanism achieving efficient mold compounding and a working method of the vertical injection molding mechanism. The vertical injection molding mechanism comprises a main box body. A lower supporting plate is fixedly mounted at the bottom of an inner cavity of the main box body. Three longitudinally-arranged guide rods are fixed to the upper endface of the lower supporting plate. The three guide rods penetrate through an injection molding table. A gear disc is rotationally arranged on the lower surface of the injection molding table, and arotating disc is fixedly embedded to the inner ring of the gear disc. Through two symmetrically-arranged second air cylinders and two symmetrically-arranged sliding rods, a hydraulic cylinder and an injection molding pipe are driven to be more balanced in the process of moving downwards; spring rods at the bottoms of sleeves contract when being in contact with the upper surface of an upper supporting plate, and the descending process of the injection molding pipe is buffered; and the technical problems that in the prior art, the vibration amplitude in the injection molding process of an injection molding machine is large, consequently, deviation happens to the injection molding process, the residual rate of injection molding raw materials is large in the prior art, the injection molding raw materials cannot be fully utilized, and meanwhile an injection molding cavity can be possibly jammed are solved.
Owner:ANHUI TENGYUE ALUMINUM PLASTIC

Injection molding machine

InactiveUS20070077327A1Stable and highly accurate injectionImprove accuracyOptical articlesAuxillary shaping apparatusComposite materialNozzle
An injection molding comprises: a fixed mold; a movable mold which is capable of contacting to and separating from the fixed mold; and an injection unit which supplies molten resin to a space formed between the fixed mold and the movable mold when being pressed to a non-molding face of the fixed mold. The injection unit comprises: a nozzle portion which injects molten resin to the space formed between the molds; an injecting portion which applies molten resin pressure toward the space formed between the fixed mold and the movable mold though the nozzle portion; a heater and a temperature sensor provided on the nozzle portion; and a heater and a temperature sensor provided on the injecting portion, and detection accuracy of the sensor of the nozzle portion is higher than that of the injecting potion. There is thus provided an injection molding machine capable of manufacturing optics with high accuracy without considerable cost-up by realizing stable and highly accurate injection.
Owner:KONICA MINOLTA OPTO

Organic electroluminescent element and its manufacturing method

The prsent invention provides an organic EL device which drives over a wide range from low brightness to high brightness for light source applications, and it operates stably over a wide range of brightness and has excellent life property is provided. The said device comprises at least one pair of electrodes 2 and 5, and a plurality of functional layers disposed between the electrodes 2 and 5, the functional layers comprising a layer 4 having the light emitting function, which is composed of at least one polymeric material and contains an organic solvent and a charge injection layer 3 composed of at least one inorganic material.
Owner:PANASONIC CORP

Gun head of fire-fighting water cannon

InactiveCN109350897AIncrease rangeImprove shot heightFire rescueSpray nozzleWater flow
The invention discloses a gun head of a fire-fighting water cannon. The gun head comprises a gun head shell, an inner nozzle, a spray core, a first flow stabilizer and a second flow stabilizer, wherein the gun head shell is internally provided with a water flow channel; the first flow stabilizer is arranged at the water flow inlet of the gun head shell, the second flow stabilizer abuts against theinterior of the gun head shell through the inner nozzle, the spray core is arranged at the water outlet of the gun head shell, and the tail end of the spray core is inserted into the inner nozzle; the head end of the inner nozzle is provided with an inclined outlet, wherein the outlet area of the inclined outlet is gradually enlarged along the spraying direction, the head end of the spray core can block the inclined outlet, and an elastic part is arranged inside the spray core; the spray core can overcome the elastic force of the elastic part and move relative to the water flow channel when being subjected to the water pressure action, so that the overflowing area of the water outlet is changed; and the structure of the double-flow-stabilizer enables the water flow to be more stable whenthe water flow is sprayed out of the fire-fighting water cannon, the water spray flow cannot be dissipated, and the range and the shooting height of the fire-fighting water cannon are improved; and the self-adaption of the size of the opening is achieved under the action of the water pressure and the elastic part, so that the self-adaptability of the water cannon can be better.
Owner:YANSHAN UNIV

Injection device and injection method for automatic intravenous injection

The invention discloses an injection device and injection method for automatic intravenous injection. The device includes a syringe for injection, a front cylinder, a piston, a piston rod, a motor system and a rear cylinder. The front cylinder is used for fixing the syringe, the internal diameter of the front cylinder is the same as the external diameter of the syringe and smaller than that of therear cylinder; a piston handle of the syringe is mounted on the piston, the piston rod is used for pushing the piston to move through the control of the motor system; the motor system is used for controlling the syringe to inject or absorb medicines according to a control instruction; the internal diameter of the rear cylinder is the same as the diameter of the piston, and the rear cylinder is used for movement of the piston. The injection device is of a double-layer piston structure to reduce the sensitivity of the piston and keep the piston in a stable and uniform injection; through an automatic intravenous injection method, a nurse does not need to participate, the operation saves time and labor, and the labor burden of medical workers is reduced.
Owner:青岛市中医医院

Preparation method of CO2 plastic foaming agent and injection system of CO2 plastic foaming agent

The invention discloses a preparation method of a CO2 plastic foaming agent and an injection system of a CO2 plastic foaming agent. The foaming agent is prepared from 3-4 parts of liquid-state CO2 and 1-2 parts of food-grade ethanol by weight. The preparation method comprises the following steps of: enabling CO2 to pass through a refrigeration system to enter a high-pressure membrane low-temperature injection pump from a CO2 liquid storage tank, and metering and proportioning the CO2 through a CO2 flow data sensor to enter a static mixer; enabling ethanol to enter a high-pressure injection pump from an ethanol storage tank, and metering and proportioning the ethanol through an ethanol flow data sensor to enter a static mixer; and fully mixing CO2 and the food-grade ethanol by static mixers to enter an energy storage tank; and intermittently injecting the mixed liquid into a foaming agent machine body by a one-way injection valve to produce the CO2 plastic foaming agent. In the invention, the injection system and the injection method of the CO2 plastic foaming agent can completely meet the technological requirements on component proportions, accurate metering, intermittent and stable injection and the like required by the CO2 foaming agent.
Owner:刘志辉

Preparation method of multiple acellular materials modified with nucleus pulposus cells for porcine small intestinal submucosa (SIS)

The invention discloses a preparation method of multiple acellular materials modified with nucleus pulposus cells for porcine small intestinal submucosa (SIS). The method specially comprises the following steps: stripping the porcine SIS tissues; carrying out treatment on the stripped porcine SIS tissues through a normal saline buffer solution containing a protease inhibitor, an organic solvent solution, a PBS (Phosphate Buffered Saline) buffer solution containing Triton X, a PBS buffer solution containing SDS (Sodium Dodecyl Sulfate) and a PBS buffer solution containing DNA enzyme to obtain acellular SIS materials; performing ball milling and pulverization on the acellular SIS materials to obtain micro-particles with diameter of 200mu m; carrying out co-culture on the micro-particles andseparated nucleus pulposus cells and then carrying out decellularization treatment again so as to obtain injectable multiple acellular nucleus pulposus repairing materials. According to the preparation method of the multiple acellular materials modified with the nucleus pulposus cells for the porcine SIS disclosed by the invention, the completeness of original ECM (Extra Cellular Matrix) can be preserved while allogeneic cells or heterologous cells with immunoreactivity are removed at the same time, and the preparation method of the multiple acellular materials modified with the nucleus pulposus cells for the porcine SIS has an good extracellular micro-environment, bio-factors, biomechanical properties and the like, and can simulate a growth environment of nucleus pulposus cells in normalphysiological conditions to a maximum limit.
Owner:ZHEJIANG UNIV
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