4results about How to "Avoid exclusion" patented technology

The invention relates to a construction method of an OriCiro cell-free cloning system based on an RCR technology, and belongs to the technical field of molecular biology. The construction method comprises the following steps: expression and purification of high-activity Tn5 transposase, transposon-mediated oriC insertion, RCR amplification, traceless excision of oriC transposon and repair cyclization. The construction method provided by the invention provides a rapid, reliable and mutation-burden-free new genome construction and rescue path for the existing research, can solve the difficult problem of construction of large-size and toxic DNA sequence vectors for gene therapy, shortens the research and development and production periods, and improves the production efficiency. Therefore, a powerful tool is provided for the fields of preparation of mRNA vaccines or therapy plasmids, construction of gene therapy vectors, supply of pharmaceutical-grade plasmid DNA, research of a virus molecular mechanism and the like.

The application discloses a casting forming die for an oil collecting shell and belongs to the technical field of thin-wall part casting devices. The die comprises a rear seat and a front seat which are connected with each other. An inner cavity die with an outer contour consistent with the shape of an inner cavity of a shell to be formed is arranged in the middle of the rear seat. A sprue, a front section riser, a middle section riser and a rear section riser are formed on the front seat. One end of each of the risers extends to the upper end face of the front seat, and the other end is connected with a corresponding discharge groove of a shell die seat. The inner cavity shape of the shell die seat is consistent with the outer contour of the shell to be formed. A chill assembly is further arranged on the shell die seat, and a forming rib vent groove is further formed on the top of the shell die seat. The application effectively solves the problems of local non-forming of parts and internal organization shrinkage and porosity in the current casting production of the oil collecting shell.

The utility model provides a building outer wall line foot drainage device of reasonable structure design has safety, durability, aesthetic property. The utility model discloses a drainage groove, fixing piece and drain pipe, the fixing piece fixed setting is on the line foot upper portion, the middle part fixed setting of fixing piece has fixing piece clamping edge, and the drainage groove clamping fixed is on fixing piece clamping edge, the middle position of drainage groove is provided with the drainage hole, and the upper surface of the part of drainage groove far from the drainage hole is high, and the upper surface of the part of drainage hole near is low, the drain pipe is embedded in the line foot, and its top stretches out line foot rear and is connected with the drainage hole, and its bottom stretches out line foot.

Screening methods are provided that are based on using ELISpot assays to measure the distribution profile of maximum spot intensity or mean spot intensity of spontaneous cytokine or chemokine or the number of "low intensity" spontaneous cytokine or chemokine producing cells as a clinically relevant measure of immune function for diagnosing patients with a particular disorder based on one or more of these measurements and for treating the particular disorder in the patient. In some embodiments, the cytokine is IFN-gamma or TNF-alpha. Patients can optionally be further evaluated to determine whether they are of an immunosuppressed or hyperinflammatory immune phenotype by quantifying cytokine and / or chemokine levels in a biological sample. Representative disorders include sepsis, autoimmune disease, autoimmunity, cancer, and lymphopenia.