The invention discloses an experimental operation device for improving the immunity of a mouse. The experimental operation device comprises a base, wherein a first hydraulic cylinder is welded at thetop of the base, an output shaft of the first hydraulic cylinder is rotatably sleeved with a disc, a first locking mechanism is arranged on the disc, a second hydraulic cylinder is welded on one sideof the disc, an injection bucket is fixedly installed on an output shaft of the second hydraulic cylinder and is of a hollow structure, a push rod is arranged above the injection bucket, one end of the push rod extends into the injection bucket, a piston is fixedly installed at the end, located in the injection bucket, of the push rod, the piston is in tight contact with the inner wall of the injection bucket, a needle tube is arranged below the injection bucket, one end of the needle tube extends into the injection bucket, and a buffer device is arranged in the injection bucket. The experimental operation device is simple in structure and convenient to operate, can fix the mouse on the base, mechanically controls the push rod for injection, makes the injection more stable and prevents muscle hypertonicity from causing medicine rejection.