This invention discloses a
fermentation process for
Bacillus subtilis, comprising: adding
Bacillus subtilis seed culture to a fermenter containing a culture medium, and real-time collecting the real-time temperature, real-time dissolved
oxygen content, and real-time pH of the culture medium; determining a first introduction rate of
carbon dioxide gas in response to a real-time pH greater than 7; determining a second introduction rate of air based on the real-time dissolved
oxygen content; determining a first introduction temperature of
carbon dioxide gas and air based on the real-time temperature, the first introduction rate, and the second introduction rate; determining a first stirring speed of the stirrer based on the first and second introduction rates; introducing the first introduction rate of
carbon dioxide gas and the second introduction rate of air into the culture medium after heating or cooling to the first introduction temperature, and controlling the stirrer to stir at the first stirring speed; determining that
fermentation is complete when the content of the target product in the culture medium reaches a threshold. This invention simplifies the
fermentation environment adjustment steps and reduces resource waste.