Yersinia pestis ghost, preparation method thereof, and application of Yersinia pestis ghost serving as vaccine

A technology of Yersinia and plague, applied in the fields of molecular biology and immunology, can solve the problems of high side reaction rate, poor production safety, and single ingredient, and achieve simple and easy preparation process, reduced possibility, strong The effect of immune memory
CN101979504AInactive Publication Date: 2011-02-23MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
Publication Date
2011-02-23
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a Yersinia pestis ghost, a preparation method thereof, and application of the Yersinia pestis ghost serving as a vaccine. The ghost has a complete outer membrane structure, can induce an organism to generate a protective antibody by inoculating a mouse without an adjuvant, generate strong immune memory and protect the mouse from being attacked by strong dose of plague live strains, and has higher safety compared with a live vaccine.
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Description

technical field

[0001] The invention relates to a Yersinia pestis slough, its preparation method and its application as a molecular delivery system, belonging to the technical fields of molecular biology and immunology. Background technique

[0002] Bacterial Ghost (BG) is a complete bacterial empty shell that induces the expression of the E gene of phage PhiX174 in Gram-negative bacteria, and finally lyses the host cell. The PhiX174E gene encodes a hydrophobic protein consisting of 91 amino acids. This protein itself has no enzymatic activity, but can connect the inner and outer membranes through oligomerization. Electron micrographs show that the inner and outer membranes fuse at the channel and form a closed periplasmic space. The diameter of the cleavage channel ranges between 40nm and 200nm. The size of the lysis channel is affected by individual differences in cells, mainly related to the degree of cell autolysis, the size of the sieve holes on the peptidoglycan laye...

Claims

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