Real-time observation method for phytophthora nicotianae infection process

A technology of Phytophthora tabacum and tobacco, which is applied in the field of research on the interaction between bacteria and plants, can solve the problem of not much tobacco, and achieve the effects of rapid onset, clear technical principles, and simple sampling

Inactive Publication Date: 2015-03-11
YUNNAN ACAD OF TOBACCO AGRI SCI
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Problems solved by technology

But so far, the process by which Phytophthor...

Method used

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  • Real-time observation method for phytophthora nicotianae infection process
  • Real-time observation method for phytophthora nicotianae infection process
  • Real-time observation method for phytophthora nicotianae infection process

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preparation example Construction

[0019] B. Preparation of Phytophthora nicotiana zoospore suspension: inoculate Phytophthora nicotiana onto OA medium plate, culture in dark at 25-28°C for 7-10 days until the mycelia cover the whole plate, take mycelia and cut into small pieces block to obtain mycelium block, liquid culture induces zoosporangium, low temperature promotes zoosporangium to release zoospore Phytophthora, adjusts zoospore concentration to be 10 with sterile water 4~6 Individual / ml obtains Phytophthora nicotiana zoospore suspension;

[0020] C, root irrigation inoculation; Get the Phytophthora nicotiana zoospore suspension that B step obtains and slowly drop to the germination bed, allow it to penetrate into the root of each cotyledon seedling;

[0021] D, dyeing observation: the cotyledon seedling after inoculation is continued to cultivate under relative humidity 80~85%, light intensity 1000~2000Lx, temperature 25~28 ℃, gets cotyledon seedling every 4 hours, removes cotyledon and cotyledon conn...

Embodiment 1

[0031] In this example, Phytophthora nicotiana physiological race No. 0 (the tobacco industry standard of the People's Republic of China YC / T39-1996) was used as the experimental strain, and the disease-resistant variety RBST and the susceptible variety Honghuadajinyuan were used as the experimental tobacco varieties.

[0032] Combined process figure 1 , the specific operation is as follows:

[0033] (1) Preparation of tobacco cotyledon seedlings: After surface disinfection of the resistant variety RBST and the susceptible variety Honghua Dajinyuan tobacco seeds, 10×10 square arrays were sown on the germination bed of a petri dish made of sponge and filter paper, at a relative humidity of 80 %-85%, light intensity 1000Lx-2000Lx, temperature 25°C-28°C in an artificial climate box, after 7-10 days of cultivation, germinate to form cotyledon seedlings, and set aside;

[0034] (2) Preparation of Phytophthora nicotiana zoospore suspension: inoculate Phytophthora nicotiana onto ...

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Abstract

The invention discloses a real-time observation method for phytophthora nicotianae infection process. The real-time observation method for the phytophthora nicotianae infection process includes that preparing for tobacco seed plantlet, preparing phytophthora nicotianae zoospore suspension, irrigating the root to inoculate, dyeing and observing. According to the real-time observation method for the phytophthora nicotianae infection process, phytophthora nicotianae inoculation can be performed in a manual climatic box or a culture room by means of culture dish sponge and filter paper for preserving moisture, and the dyed and discolored seed plantlet radicle can be directly observed under a microscope. The real-time observation method for the phytophthora nicotianae infection process is convenient and easy to operate and suitable for observing the phytophthora nicotianae infection process of tobacco and detecting the resistance of different varieties.

Description

technical field [0001] The invention belongs to the technical field of research on the interaction between pathogens and plants, and in particular relates to a real-time observation method for the infection process of Phytophthora nicotiana. Background technique [0002] Tobacco black shank is caused by Phytophthora nicotianae ( Phytophthora nicotianae ) is a soil-borne disease, which is one of the main diseases affecting the yield and quality of tobacco. Studies have shown that Phytophthora nicotianae is active within 5 cm from the soil surface, and the infection sites are mainly stem bases and roots. Bacteria-carrying soil, manure and irrigation water are the main sources of primary infection of tobacco black shank, followed by disease-carrying tobacco seedlings. The sporangia and zoospores produced by it are spread by rainwater or irrigation water, and can pass through stomata, wounds or piercings. Invasion of the stratum corneum through tender epidermal cells causes re...

Claims

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Application Information

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IPC IPC(8): A01G7/06
CPCA01G7/06
Inventor 方敦煌肖炳光陈学军姚恒童治军吴劲松
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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