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Biocontrol strain BCJB01 and application thereof

A biocontrol strain, the technology of CGMCCNO.10477, is applied in the direction of application, bacteria, and biocides, and can solve problems such as endangering human health, declining fruit quality, and increasing costs.

Inactive Publication Date: 2015-06-03
BIOLOGY INST OF SHANDONG ACAD OF SCI +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Since the invention of Bordeaux mixture in 1882, chemical prevention and control has always been the most important measure to control the hazards, prevalence, and disasters of downy mildew. Most fruit and vegetable production areas require multiple and large-scale applications of chemical agents to successfully prevent and control its hazards and disasters , which also brings cost increase, fruit quality decline, harm to human health and damage to ecological balance and other problems (Li Hua, Guo Minghao

Method used

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  • Biocontrol strain BCJB01 and application thereof
  • Biocontrol strain BCJB01 and application thereof
  • Biocontrol strain BCJB01 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Study on Degradation of Zoospores of Grape Downy Mildew by Biocontrol Strain BCJB01

[0020] Cultivate the sporangia of grape downy mildew under suitable conditions to germinate, obtain zoospores, and quantify them to 2×10 5 CFU mL -1 ,spare. After the biocontrol strain BCJB01 was fermented and cultivated, the bacterial cell suspension (4×10 8 CFU mL -1 ), fermented liquid supernatant, 10-fold diluted fermented liquid, 50-fold diluted fermented liquid and 100-fold diluted fermented liquid equal amount join in quantitative downy mildew zoospore liquid, research its effect on grape downy mildew zoospore of degradation. The results are shown in Table 3: in the samples containing bacteria, the number of zoospores decreased with the prolongation of treatment time. When the BCJB01 fermentation broth was diluted to 100 times, the number of zoospores still decreased with the prolongation of treatment time, and at 4.5 hours after treatment, the number of zoospores was chang...

Embodiment 2

[0024] Control of Downy Mildew by Biocontrol Strain BCJB01

[0025] 1 Determination of the inhibitory effect of biocontrol strain BCJB01 on grape downy mildew by leaf disc method

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Abstract

The invention discloses a biocontrol strain BCJB01 and application thereof. Bacillus cereus BCJB01 is preserved in China General Microbiological Culture Collection Center on January 30, 2015 with the preservation number to be CGMCC NO. 10477. The Bacillus cereus BCJB01 has the unique action of decomposing downy mildew zoospores, and thus having good controlling effect to both grape downy mildew and cucumber downy mildew.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a biocontrol strain BCJB01 and its application. Background technique [0002] Cucumber downy mildew is caused by Cuban Pseudoperonospora cubensis (Berk.et Curt.) Rostov, which is one of the main leaf diseases in cucumber producing areas all over the world. This disease has occurred in more than 70 countries in the world, which is a great threat to cucumber production. The light ones lose 10% to 20%, and the severe ones 30%, or even no harvest, seriously affecting the yield and quality of cucumbers. The pathogenic fungus asexually reproduces well, and sporangia are produced by sporangia. The sporangia can release 1 to 8 zoospores at low temperature, and invade the host from the stomata or intercellular space (Niu De, Fu Jia, etc. Cucumber downy mildew research New progress [J]. Journal of Northeast Forestry University, 2008, 36(9): 94-98; Zhu Jinying, Wang Youping, etc. Research prog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/085
CPCA01N63/10C12N1/205C12R2001/085
Inventor 谢雪迎扈进冬赵玖华李纪顺杨合同王贻莲
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
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