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High-efficiency biocontrol strain screening method taking pepper phytophthora blight as target

A technology for biocontrol of strains and pepper blight, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of slow progress in the biological control of pepper blight, difficulty in screening, etc. Small space, easy to buy effect

Inactive Publication Date: 2014-08-13
텐진인스티튜트오브플랜트프로텍션
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For a long time, the progress of biological control of pepper blight has been slow, and the difficulty in the sc

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A high-efficiency screening method for biocontrol strains targeting pepper blight:

[0035] (1) Seed disinfection: Rinse the required number of pepper seeds with sterile water, and then treat them with 75% alcohol and 5% NaClO for 30s and 5min respectively, and the selected pepper variety is improved 8819 line pepper;

[0036] (2) Seed germination: Soak the sterilized seeds in sterile water for 4 hours, then put them into a petri dish covered with wet gauze, and put them in an incubator at 25°C in the dark for 48 hours to accelerate germination to white;

[0037] (3) Substrate sterilization: put 30 grams of ceramsite with a diameter of 2 mm into the tissue culture bottle, the height of the ceramsite is 1 / 3 of the height of the tissue culture bottle, add 40 mL of plant nutrient solution, and seal it with a sealing film; Bacteria, 121°C, 30min; tissue culture bottle height 100mm, caliber 50mm, outer diameter 60mm;

[0038] (4) Seed soaking of bio-control bacteria: pick ...

Embodiment 2

[0045] A high-efficiency screening method for biocontrol strains targeting pepper blight:

[0046] (1) Seed disinfection: Rinse the required number of pepper seeds with sterile water, and then treat them with 75% alcohol and 5% NaClO for 30s and 5min respectively, and the selected pepper variety is improved 8819 line pepper;

[0047] (2) Seed germination: Soak the sterilized seeds in sterile water for 4 hours, then put them into a petri dish covered with wet gauze, and put them in an incubator at 25°C in the dark for 48 hours to accelerate germination to white;

[0048] (3) Matrix sterilization: Put 30 grams of ceramsite with a diameter of 8 mm into a tissue culture bottle. The height of the tissue culture bottle is 100 mm, the diameter is 50 mm, and the outer diameter is 60 mm. The height of the ceramsite is 1 / 3 of the height of the tissue culture bottle, add 40mL of plant nutrient solution, and seal it with a sealing film; sterilize with damp heat, 121°C, 30min; the preparat...

Embodiment 3

[0057] Actual application

[0058] The control effect of 7 strains of self-sieving biocontrol bacteria on pepper blight was determined indoors.

[0059] Phytophthora capsici ( Phytophthora capsici ), Rhizoctonia solani ( Rhizoctonia solani ), Botrytis cinerea ( Botrytis cinerea ) and Pythium melon ( Pythium aphanidermatum ) Four kinds of soil-borne disease pathogenic bacteria are provided by the plant disease and biological control room of Tianjin Plant Protection Research Institute (it can be provided free of charge to the outside world).

[0060] 7 self-screening strains (TB1309, TB1312, TB1317, TB1321, TB1335, TB1340, TB1348)

[0061] The preparation method is as follows:

[0062] (1) More than 10,000 strains of bacteria were isolated from vegetable rhizosphere soils in Tianjin, Hebei, Shandong, Shanxi, Fujian, Henan and Jiangsu;

[0063] (2) Screen out the pathogens against Phytophthora capsici ( Phytophthora capsici ) 802 bacterial strains with obvious antagonis...

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Abstract

The invention discloses a high-efficiency biocontrol strain screening method taking pepper phytophthora blight as a target. The high-efficiency biocontrol strain screening method comprises the following steps: putting 30g of ceramsite in a tissue culture vessel, adding 40ml of plant nutrient solution to be right over the ceramsite, planting germinated pepper seeds, subjected to biocontrol seed soaking treatment, on the ceramsite, and inoculating 5ml of zoospore suspension liquid with the adjustment concentration of 105mL<-1> below the level of the nutrient solution after the pepper grows to have four main leaves. The zoospore suspension liquid is obtained by carrying out low-temperature induction after intermittently irradiating phytophthora capsici on a CA culture medium for 6-8 days. The application range of the high-efficiency biocontrol strain screening method includes screening of biocontrol strain taking pepper phytophthora blight as a target, and the like. The method is simple to operate and short in the period from seedling to inoculation, the zoospore inoculation can be conveniently implemented and observed, and the method has practical guidance significance in the biological control research work of plant diseases.

Description

technical field [0001] The invention belongs to the technical field of agricultural plant protection, and relates to a screening method for various crop blight epidemics, in particular to a high-efficiency screening method for biocontrol bacterial strains targeting pepper blight. Background technique [0002] Pepper blight is caused by Phytophthora capsici ( Phytophthora capsici Leonian) is a worldwide soil-borne disease that occurs in all pepper producing areas. In addition to harming peppers, its pathogenic bacteria can also infect more than 20 kinds of crops such as zucchini, cucumbers, pumpkins, eggplants, and tomatoes. The disease was first reported in New Mexico, USA in 1918 and is now widespread worldwide. In my country, the disease was first discovered in Jiangsu in the 1960s by Yu Dafu. So far, pepper blight has occurred in Xinjiang, Hebei, Heilongjiang, Guizhou, Guangzhou, Gansu, Yunnan, Shanghai and Shaanxi. In addition to China, it has occurred in France, Aus...

Claims

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Application Information

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IPC IPC(8): C12Q1/04C12R1/645
Inventor 田涛孙冰冰李伟魏军
Owner 텐진인스티튜트오브플랜트프로텍션
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